Construct: sgRNA BRDN0001147198
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGTCACTGATGTAAATGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHB2 (2048)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77548
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
22865086 |
- |
EPHB2 |
NNNTCACTGATGTAAATGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
32678913 |
- |
TTC27 |
NNNTCACTGATGTAAATGCC |
NGA |
2 |
0.0298 |
Tier I |
| 3 |
NC_000002.12 |
2 |
10819439 |
- |
PDIA6 |
NNNTCACAGATGTAAATACG |
NGG |
2 |
0.5538 |
Tier II |
| 4 |
NC_000022.11 |
22 |
28414632 |
- |
TTC28 |
NNNTCACTAATGTAAATGCT |
NGG |
2 |
0.45 |
Tier II |
| 5 |
NC_000003.12 |
3 |
192471559 |
+ |
FGF12 |
NNNTCACTGATTTAAATGCT |
NGG |
2 |
0.2692 |
Tier II |
| 6 |
NC_000016.10 |
16 |
49290718 |
- |
LOC102724859 |
NNNTCACTGATGTAAAGGAG |
NGG |
2 |
0.0504 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
136693862 |
+ |
Ephb2 |
NNNTCACTGATGTAGATGCG |
NGG |
1 |
0.65 |
Tier I |
| 2 |
NC_000077.6 |
11 |
20229573 |
+ |
Cep68 |
NNNTCACTGATGCAAATGCA |
NGG |
2 |
0.7401 |
Tier II |
| 3 |
NC_000073.6 |
7 |
55172817 |
+ |
Luzp2 |
NNNTCACTGATTTAAATGCT |
NGG |
2 |
0.2692 |
Tier II |
| 4 |
NC_000067.6 |
1 |
151629646 |
- |
Niban1 |
NNNTCACTGAGGTAAATGAG |
NGG |
2 |
0.1714 |
Tier II |
| 5 |
NC_000070.6 |
4 |
100955637 |
- |
Cachd1 |
NNNTCACTGCTGTAAATGAG |
NGG |
2 |
0.1429 |
Tier II |
| 6 |
NC_000083.6 |
17 |
22364740 |
- |
Zfp758 |
NNNTCAGTGATGTAAATGGG |
NGG |
2 |
0.0588 |
Tier II |
Other clones with same target sequence:
(none)
