Construct: sgRNA BRDN0001147200
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACGTAAGATCATCCGCATGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RIOK3 (8780)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76539
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000018.10 |
18 |
23473593 |
+ |
RIOK3 |
NNNTAAGATCATCCGCATGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
56184575 |
- |
ANKFN1 |
NNNTAATATCATCTGCATGT |
NGG |
2 |
0.419 |
Tier II |
| 3 |
NC_000015.10 |
15 |
39997322 |
+ |
EIF2AK4 |
NNNAAAGATCATCAGCATGT |
NGG |
2 |
0.1667 |
Tier II |
| 4 |
NC_000002.12 |
2 |
29911831 |
+ |
ALK |
NNNTAAGATCATCCAGATGT |
NGG |
2 |
0.1448 |
Tier II |
| 5 |
NC_000015.10 |
15 |
71840371 |
- |
MYO9A |
NNNTAAGATCATCCTCGTGT |
NGG |
2 |
0.0252 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
12148955 |
+ |
Riok3 |
NNNTAAGATCATCCGCATGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000080.6 |
14 |
33182912 |
+ |
Wdfy4 |
NNNTAAAATGATCCGCATGT |
NGG |
2 |
0.3889 |
Tier II |
| 3 |
NC_000068.7 |
2 |
91065349 |
- |
Slc39a13 |
NNNTAAGATCAGCTGCATGT |
NGG |
2 |
0.3667 |
Tier II |
| 4 |
NC_000074.6 |
8 |
77363181 |
- |
Arhgap10 |
NNNTAAGTTCATCTGCATGT |
NGG |
2 |
0.3143 |
Tier II |
| 5 |
NC_000082.6 |
16 |
17351873 |
- |
Pi4ka |
NNNTAAGATCATCCACATGT |
NAG |
2 |
0.244 |
Tier II |
Other clones with same target sequence:
(none)
