Construct: sgRNA BRDN0001147203

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACCAGAATGATGCCCTCTGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MPP4 (58538)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77114

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 201680912 - MPP4 NNNAGAATGATGCCCTCTGG NGG 0 1.0 Tier I
2 NC_000012.12 12 24836571 + BCAT1 NNNAGAATGATGCCATCTAG NGG 2 0.1587 Tier I
3 NC_000006.12 6 32118986 - ATF6B NNNAGAATGCTGCCCTCCGG NGG 2 0.1429 Tier I
4 NC_000014.9 14 88291710 + KCNK10 NNNTGAATGATGCCCTCTGG NGG 1 0.6364 Tier II
5 NC_000011.10 11 83873986 + DLG2 NNNACAATGAAGCCCTCTGG NGG 2 0.5893 Tier II
6 NC_000013.11 13 51234342 - FAM124A NNNAGAATGGTGCTCTCTGG NGG 2 0.4074 Tier II
7 NC_000007.14 7 43211518 - HECW1 NNNGGAATCATGCCCTCTGG NGG 2 0.3365 Tier II
8 NC_000009.12 9 93112518 + CARD19 NNNAGAATGATGACCTCTAG NGG 2 0.2747 Tier II
9 NC_000005.10 5 170791547 + GABRP NNNAGAAAGCTGCCCTCTGG NGG 2 0.2667 Tier II
10 NC_000001.11 1 153744541 - INTS3 NNNAGAATGACTCCCTCTGG NGG 2 0.2564 Tier II
11 NC_000011.10 11 65593974 + KCNK7 NNNAGAAGGCTGCCCTCTGG NGG 2 0.2444 Tier II
12 NC_000011.10 11 7569894 + PPFIBP2 NNNAGGATGATGCCCTCGGG NGG 2 0.2381 Tier II
13 NC_000014.9 14 70074140 + SLC8A3 NNNAGAATGCTGCCCCCTGG NGG 2 0.2222 Tier II
14 NC_000006.12 6 34055429 - GRM4 NNNAGCATGCTGCCCTCTGG NGG 2 0.1515 Tier II
15 NC_000021.9 21 33899097 + ITSN1 NNNAGAACGATGCCCTCTGG NTG 2 0.039 Tier II
16 NC_000005.10 5 97132955 - LIX1 NNNAGTATGATGCCCTCTGG NTG 2 0.0278 Tier II
17 NC_000023.11 X 31712354 + DMD NNNAGAATGTTGCCCTCTGG NGT 2 0.0142 Tier II
18 NC_000007.14 7 136874625 + CHRM2 NNNTGAATGATGCCCTCTGG NGT 2 0.0103 Tier II
19 NC_000009.12 9 112848683 - SNX30 NNNAGAATGCTGCCCTCTGG NGC 2 0.0074 Tier II
20 NC_000016.10 16 78638954 + WWOX NNNAGAATGATTCCCTCTGG NGT 2 0.0062 Tier II
21 NC_000009.12 9 108996438 - CTNNAL1 NNNAGAATGATGCCTTCTGG NGT 2 0.0011 Tier II
22 NC_000012.12 12 498431 + B4GALNT3 NNNAGAATGATGCCCTCTGG NCC 2 0.0 Tier II
23 NC_000006.12 6 56347118 + COL21A1 NNNAGAATGATGCGCTCTGT NGG 2 0.0 Tier II
24 NC_000007.14 7 31179983 - LOC107986781 NNNAGAGTGATGCTCTCTGG NGG 2 0.5176 Tier III
25 NC_000006.12 6 105883922 - LOC105377923 NNNAGACTGAAGCCCTCTGG NGG 2 0.3281 Tier III
26 NC_000011.10 11 7569894 + LOC105376535 NNNAGGATGATGCCCTCGGG NGG 2 0.2381 Tier III
27 NC_000002.12 2 103963319 - LINC01965 NNNAGAATGAGGCCCTCTGG NAG 2 0.1037 Tier III
28 NC_000007.14 7 119773708 + LINC02476 NNNAGATTGATGCCTTCTGG NGG 2 0.0292 Tier III
29 NC_000013.11 13 113277598 + LDHBP1 NNNAGAATGAAGCCCTCTGG NGC 2 0.0167 Tier III
30 NC_000009.12 9 108996438 - LOC105376216 NNNAGAATGATGCCTTCTGG NGT 2 0.0011 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 59144728 - Mpp4 NNNAGAATGATGCCCTCTGG NGG 0 1.0 Tier I
2 NC_000072.6 6 145010110 + Bcat1 NNNAGAATGATGCCATCTAG NGG 2 0.1587 Tier I
3 NC_000074.6 8 88252989 - Tent4b NNNACAAGGATGCCCTCTGG NGG 2 0.5762 Tier II
4 NC_000072.6 6 83187459 + Dctn1 NNNAGAAGGATGCTCTCTGG NGG 2 0.5378 Tier II
5 NC_000078.6 12 76199557 - Tex21 NNNTGAAAGATGCCCTCTGG NGG 2 0.5091 Tier II
6 NC_000074.6 8 12628285 - Tubgcp3 NNNAGAGTGATGTCCTCTGG NGG 2 0.4941 Tier II
7 NC_000080.6 14 29516710 - Cacna2d3 NNNAGAATGATGCTCTCTTG NGG 2 0.4889 Tier II
8 NC_000068.7 2 73572627 - Chrna1 NNNAAAATGGTGCCCTCTGG NGG 2 0.4815 Tier II
9 NC_000085.6 19 42306158 + Crtac1 NNNAGAACGATGCCCTTTGG NGG 2 0.4667 Tier II
10 NC_000077.6 11 33570604 - Gabrp NNNAGAAAGGTGCCCTCTGG NGG 2 0.4444 Tier II
11 NC_000079.6 13 93084140 - Cmya5 NNNAGACTGATGCCCTCTGA NGG 2 0.4102 Tier II
12 NC_000085.6 19 31944064 + A1cf NNNAGATTGATGCCCTCTGT NGG 2 0.3062 Tier II
13 NC_000085.6 19 6998481 - Trpt1 NNNAGAATGAGGCTCTCTGG NGG 2 0.2933 Tier II
14 NC_000076.6 10 100456022 + Tmtc3 NNNAGAATGATGCCATCTGG NGG 1 0.2222 Tier II
15 NC_000068.7 2 181511986 - Tpd52l2 NNNAGAATGGTTCCCTCTGG NGG 2 0.2137 Tier II
16 NC_000068.7 2 144653852 + Dtd1 NNNAGAATGAGGCCCTTTGG NGG 2 0.1867 Tier II
17 NC_000072.6 6 91751168 + Slc6a6 NNNAGAATGAGGCCCTCTGC NGG 2 0.1714 Tier II
18 NC_000073.6 7 49462924 + Nav2 NNNAGAATGATGCCACCTGG NGG 2 0.1481 Tier II
19 NC_000079.6 13 11622879 + Ryr2 NNNAGAATGGTGCCCTCTGG NAG 2 0.144 Tier II
20 NC_000072.6 6 48649527 - Gimap8 NNNAGATTGATGCCATCTGG NGG 2 0.0972 Tier II
21 NC_000077.6 11 22901982 - Commd1 NNNAGAATGAAGCCCTGTGG NGG 2 0.0441 Tier II
22 NC_000078.6 12 87163374 - Gstz1 NNNAGCATGATGCCTTCTGG NGG 2 0.0303 Tier II
23 NC_000077.6 11 80260370 + Rhot1 NNNAGAATGAGGCCCTGTGG NGG 2 0.0235 Tier II
24 NC_000077.6 11 86717065 + Cltc NNNAGAATGATGCCTTCTGG NAG 2 0.0173 Tier II
25 NC_000067.6 1 173371769 - Aim2 NNNAGAATGATGCCCCCTGG NGT 2 0.0108 Tier II
26 NC_000073.6 7 80648118 + Crtc3 NNNAGAATGATGGCTTCTGG NGG 2 0.0091 Tier II
27 NC_000079.6 13 74055174 + Cep72 NNNAGAATGATGGGCTCTGG NGG 2 0.0 Tier II
28 NC_000085.6 19 42306158 + Gm34347 NNNAGAACGATGCCCTTTGG NGG 2 0.4667 Tier III
29 NC_000079.6 13 110368734 + Gm41058 NNNAGAATGCTGCCCTCTGC NGG 2 0.1429 Tier III
30 NC_000079.6 13 110368783 + Gm41058 NNNAGAATGCTGCCCTCTGC NGG 2 0.1429 Tier III
31 NC_000079.6 13 110368832 + Gm41058 NNNAGAATGCTGCCCTCTGC NGG 2 0.1429 Tier III
32 NC_000079.6 13 110368881 + Gm41058 NNNAGAATGCTGCCCTCTGC NGG 2 0.1429 Tier III
33 NC_000085.6 19 26540339 + Gm52395 NNNAGAATGATTCCCTCTGG NCG 2 0.0412 Tier III
34 NC_000073.6 7 80648118 + Gm51450 NNNAGAATGATGGCTTCTGG NGG 2 0.0091 Tier III
35 NC_000070.6 4 4151386 + A830012C17Rik NNNAGAATGATGCCCTCTGG NTC 2 0.0 Tier III
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Other clones with same target sequence:

(none)