Construct: sgRNA BRDN0001147207

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAGAACGTGTAGCAATAAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK39 (27347)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76072

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 168182026 - STK39 NNNAACGTGTAGCAATAAAA NGG 0 1.0 Tier I
2 NC_000018.10 18 63554116 + SERPINB12 NNNAAAATGTAGCAATAAAA NGG 2 0.9286 Tier II
3 NC_000001.11 1 246645964 - CNST NNNAAAGGGTAGCAATAAAA NGG 2 0.681 Tier II
4 NC_000005.10 5 78902565 + ARSB NNNAATGTATAGCAATAAAA NGG 2 0.5969 Tier II
5 NC_000023.11 X 133313328 + GPC4 NNNTACGTGTAACAATAAAA NGG 2 0.5939 Tier II
6 NC_000004.12 4 21907445 - KCNIP4 NNNAATTTGTAGCAATAAAA NGG 2 0.5306 Tier II
7 NC_000018.10 18 69643602 - DOK6 NNNAATGTTTAGCAATAAAA NGG 2 0.4952 Tier II
8 NC_000020.11 20 14915200 + MACROD2 NNNAAGGTGTAACAATAAAA NGG 2 0.4667 Tier II
9 NC_000021.9 21 41186010 + BACE2 NNNAAAGTGGAGCAATAAAA NGG 2 0.4643 Tier II
10 NC_000007.14 7 147123687 - CNTNAP2 NNNAAAGTGTAGCAATATAA NGG 2 0.4643 Tier II
11 NC_000001.11 1 220198477 - RAB3GAP2 NNNAATGTGTAGCAATATAA NGG 2 0.4643 Tier II
12 NC_000002.12 2 112578507 - POLR1B NNNACTGTGTAGCAATAAAA NGG 2 0.4643 Tier II
13 NC_000011.10 11 59855177 - TCN1 NNNAACGTTAAGCAATAAAA NGG 2 0.4571 Tier II
14 NC_000020.11 20 19819496 - RIN2 NNNAAAGTGTATCAATAAAA NGG 2 0.3571 Tier II
15 NC_000019.10 19 34437767 + UBA2 NNNAATGTGTATCAATAAAA NGG 2 0.3571 Tier II
16 NC_000010.11 10 87782655 + ATAD1 NNNAACCTGTAGCAAGAAAA NGG 2 0.238 Tier II
17 NC_000013.11 13 66707198 + PCDH9 NNNAACATGTAGCAATAACA NGG 2 0.2069 Tier II
18 NC_000007.14 7 120380637 - KCND2 NNNAATGTGTAGCAATAAAA NGC 2 0.0206 Tier II
19 NC_000002.12 2 227392124 + LOC124907993 NNNAACTTGAAGCAATAAAA NGG 2 0.4898 Tier III
20 NC_000020.11 20 14915200 + MACROD2-AS1 NNNAAGGTGTAACAATAAAA NGG 2 0.4667 Tier III
21 NC_000010.11 10 87782655 + LOC124902476 NNNAACCTGTAGCAAGAAAA NGG 2 0.238 Tier III
22 NC_000014.9 14 49009528 - LOC105378178 NNNTACGTGTAGCAATAACA NGG 2 0.1317 Tier III
23 NC_000002.12 2 207675205 - LINC01857 NNNAACATGTAGCAATAAAA NGT 2 0.0161 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000078.6 12 78610883 + Gphn NNNAACATGTAGTAATAAAA NGG 2 0.7 Tier II
2 NC_000086.7 X 104616052 + Zdhhc15 NNNAACCAGTAGCAATAAAA NGG 2 0.55 Tier II
3 NC_000075.6 9 53518693 - Atm NNNAAATTGTAGCAATAAAA NGG 2 0.5306 Tier II
4 NC_000081.6 15 77390630 - Apol7a NNNAATGTCTAGCAATAAAA NGG 2 0.5 Tier II
5 NC_000080.6 14 105143083 - Rbm26 NNNAAAGTGTAGAAATAAAA NGG 2 0.3571 Tier II
6 NC_000069.6 3 123537858 - Ndst3 NNNATAGTGTAGCAATAAAA NGG 2 0.3377 Tier II
7 NC_000067.6 1 176963236 + Sdccag8 NNNAACGTGGAGCAGTAAAA NGG 2 0.325 Tier II
8 NC_000086.7 X 52107637 - Gpc4 NNNAACGTGTAGCAAAAACA NGG 2 0.1881 Tier II
9 NC_000083.6 17 47293481 + Trerf1 NNNAACGTGTAGCATCAAAA NGG 2 0.1333 Tier II
10 NC_000068.7 2 33576309 + Lmx1b NNNAACGAGTAGCAATTAAA NGG 2 0.1067 Tier II
11 NC_000080.6 14 70692286 - Xpo7 NNNAACGTGTAGAAATTAAA NGG 2 0.0513 Tier II
12 NC_000071.6 5 4001173 - Akap9 NNNAACCTGTAGCAATAAAA NGA 2 0.0477 Tier II
13 NC_000079.6 13 117664003 - Hcn1 NNNAACCTGTAGCAATAAAA NGA 2 0.0477 Tier II
14 NC_000072.6 6 104745791 - Cntn6 NNNAACTTGTAGCAATAAAA NGA 2 0.0397 Tier II
15 NC_000069.6 3 136908267 - Ppp3ca NNNAACGTGTAGCAATGACA NGG 2 0.0365 Tier II
16 NC_000069.6 3 51475965 + Naa15 NNNAACTTGTAGCAATAAAA NGC 2 0.0127 Tier II
17 NC_000082.6 16 94487787 + Gm31323 NNNAACCTGTAGCTATAAAA NGG 2 0.3667 Tier III
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Other clones with same target sequence:

(none)