Construct: sgRNA BRDN0001147212
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGATTACAAAGACTGCAACT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PFKFB3 (5209)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76463
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
6219606 |
+ |
PFKFB3 |
NNNTTACAAAGACTGCAACT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
88877630 |
- |
ZNF804B |
NNNTTATAAAGACTGAAACT |
NGG |
2 |
0.8125 |
Tier II |
| 3 |
NC_000017.11 |
17 |
8096921 |
- |
ALOXE3 |
NNNTTACAATGGCTGCAACT |
NGG |
2 |
0.6373 |
Tier II |
| 4 |
NC_000002.12 |
2 |
55197532 |
+ |
CLHC1 |
NNNTCACAAGGACTGCAACT |
NGG |
2 |
0.5556 |
Tier II |
| 5 |
NC_000011.10 |
11 |
125964406 |
+ |
CDON |
NNNTTACAAAGACTGCAACT |
NAG |
1 |
0.2593 |
Tier II |
| 6 |
NC_000005.10 |
5 |
78424442 |
+ |
SCAMP1 |
NNNTTACAAAGATTGTAACT |
NGG |
2 |
0.2154 |
Tier II |
| 7 |
NC_000017.11 |
17 |
76156588 |
+ |
RNF157 |
NNNATACAAAGACTGCAGCT |
NGG |
2 |
0.1905 |
Tier II |
| 8 |
NC_000007.14 |
7 |
48548421 |
- |
ABCA13 |
NNNTAACAAAGACTTCAACT |
NGG |
2 |
0.0714 |
Tier II |
| 9 |
NC_000015.10 |
15 |
72487938 |
- |
ARIH1 |
NNNTTACAAACAGTGCAACT |
NGG |
2 |
0.0584 |
Tier II |
| 10 |
NC_000006.12 |
6 |
147317065 |
+ |
STXBP5 |
NNNTTACAAAGAATTCAACT |
NGG |
2 |
0.0549 |
Tier II |
| 11 |
NC_000001.11 |
1 |
183581606 |
+ |
NCF2 |
NNNGTACAAAGACTGCAACT |
NTG |
2 |
0.0244 |
Tier II |
| 12 |
NC_000011.10 |
11 |
125965696 |
+ |
CDON |
NNNTTACAAAGACTGCAACT |
NAA |
2 |
0.0 |
Tier II |
| 13 |
NC_000008.11 |
8 |
72237894 |
+ |
TRPA2P |
NNNTTACATAGACTGAAACT |
NGG |
2 |
0.6 |
Tier III |
| 14 |
NC_000005.10 |
5 |
124170241 |
+ |
LINC01170 |
NNNTTACAAAGACTGAAACA |
NGG |
2 |
0.5625 |
Tier III |
| 15 |
NC_000011.10 |
11 |
82801798 |
+ |
LINC02734 |
NNNTTACAAAGACTGAAAAT |
NGG |
2 |
0.4286 |
Tier III |
| 16 |
NC_000020.11 |
20 |
12388124 |
+ |
LOC124904870 |
NNNTTACAAATACTACAACT |
NGG |
2 |
0.362 |
Tier III |
| 17 |
NC_000007.14 |
7 |
52318351 |
- |
LOC107986796 |
NNNTTACAAAGACAGCAACT |
NGA |
2 |
0.043 |
Tier III |
| 18 |
NC_000007.14 |
7 |
52318351 |
- |
LOC124901810 |
NNNTTACAAAGACAGCAACT |
NGA |
2 |
0.043 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
11486307 |
- |
Pfkfb3 |
NNNTTACAAAGACTGCAACT |
NAG |
1 |
0.2593 |
Tier I |
| 2 |
NC_000076.6 |
10 |
36670672 |
- |
Hs3st5 |
NNNTTATAAAAACTGCAACT |
NGG |
2 |
0.8125 |
Tier II |
| 3 |
NC_000072.6 |
6 |
96222025 |
- |
Tafa1 |
NNNTTATAAAGACTGAAACT |
NGG |
2 |
0.8125 |
Tier II |
| 4 |
NC_000078.6 |
12 |
54189832 |
- |
Egln3 |
NNNTTGCAAAGACTGCAACT |
NGG |
1 |
0.7143 |
Tier II |
| 5 |
NC_000080.6 |
14 |
56475540 |
+ |
Rnf17 |
NNNTTACAAACACTACAACT |
NGG |
2 |
0.4034 |
Tier II |
| 6 |
NC_000077.6 |
11 |
54645275 |
+ |
Rapgef6 |
NNNTTGCAAAGACTGCAAAT |
NGG |
2 |
0.3061 |
Tier II |
| 7 |
NC_000079.6 |
13 |
67079985 |
+ |
Zfp708 |
NNNTTGCTAAGACTGCAACT |
NGG |
2 |
0.3061 |
Tier II |
| 8 |
NC_000086.7 |
X |
87798343 |
+ |
Il1rapl1 |
NNNTTACACAGACTGCAAAT |
NGG |
2 |
0.2449 |
Tier II |
| 9 |
NC_000068.7 |
2 |
66522507 |
+ |
Scn9a |
NNNTAACAAAGACTGCAATT |
NGG |
2 |
0.2308 |
Tier II |
| 10 |
NC_000071.6 |
5 |
103900402 |
+ |
Klhl8 |
NNNTTACAAAGACTGCATAT |
NGG |
2 |
0.2143 |
Tier II |
| 11 |
NC_000077.6 |
11 |
45889723 |
- |
Clint1 |
NNNCTACAAAGACTGCAACT |
NAG |
2 |
0.1678 |
Tier II |
| 12 |
NC_000082.6 |
16 |
92064790 |
- |
Slc5a3 |
NNNTTCCAAAGACTCCAACT |
NGG |
2 |
0.124 |
Tier II |
| 13 |
NC_000082.6 |
16 |
92064790 |
- |
Mrps6 |
NNNTTCCAAAGACTCCAACT |
NGG |
2 |
0.124 |
Tier II |
| 14 |
NC_000071.6 |
5 |
4039742 |
+ |
Akap9 |
NNNTTAAAAAGACTGGAACT |
NGG |
2 |
0.1154 |
Tier II |
| 15 |
NC_000068.7 |
2 |
7072038 |
+ |
Celf2 |
NNNATACAAAGACTGCAAGT |
NGG |
2 |
0.0595 |
Tier II |
| 16 |
NC_000070.6 |
4 |
57535855 |
+ |
Pakap |
NNNTTACAAAGAGTGCAGCT |
NGG |
2 |
0.0545 |
Tier II |
| 17 |
NC_000082.6 |
16 |
44107131 |
+ |
Atp6v1a |
NNNTTACAAAGACTACAACT |
NGT |
2 |
0.0152 |
Tier II |
| 18 |
NC_000086.7 |
X |
75186588 |
- |
F8 |
NNNTTACAAAGACTGCCACT |
NGA |
2 |
0.0123 |
Tier II |
| 19 |
NC_000068.7 |
2 |
140149588 |
- |
Esf1 |
NNNTTACAAAGACTGGAACT |
NGA |
2 |
0.0107 |
Tier II |
| 20 |
NC_000077.6 |
11 |
75440996 |
+ |
Wdr81 |
NNNTTACATAGACTGCAACT |
NGT |
2 |
0.0097 |
Tier II |
| 21 |
NC_000068.7 |
2 |
66522507 |
+ |
Gm13629 |
NNNTAACAAAGACTGCAATT |
NGG |
2 |
0.2308 |
Tier III |
| 22 |
NC_000068.7 |
2 |
151747632 |
- |
4930556L07Rik |
NNNTTACAAAGGCTGCCACT |
NGG |
2 |
0.1275 |
Tier III |
| 23 |
NC_000072.6 |
6 |
60341185 |
+ |
Gm35335 |
NNNTTACAAATACTGCAACT |
NAG |
2 |
0.0997 |
Tier III |
Other clones with same target sequence:
(none)
