Construct: sgRNA BRDN0001147218

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TATGGTGCGTTCTACAGCGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP2K1 (5604)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76212

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 66436859 + MAP2K1 NNNGGTGCGTTCTACAGCGA NGG 0 1.0 Tier I
2 NC_000010.11 10 110127840 - ADD3 NNNGGTGCGTTCTACAGTAA NGG 2 0.4592 Tier II
3 NC_000008.11 8 30028121 + MAP2K1P1 NNNGGTGCATTCTACAGCGA NAG 2 0.1667 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 64212618 - Map2k1 NNNGGGGCCTTCTACAGCGA NGG 2 0.3077 Tier I
2 NC_000076.6 10 81115139 + Map2k2 NNNGGGGCCTTCTACAGCGA NGG 2 0.3077 Tier I
3 NC_000077.6 11 103838526 + Nsf NNNGGTGAGTTCTACAGAGA NGG 2 0.35 Tier II
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Other clones with same target sequence:

(none)