Construct: sgRNA BRDN0001147219
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGAGATCCAACAGTACGCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CROT (54677)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77707
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
87377387 |
+ |
CROT |
NNNGATCCAACAGTACGCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
87377387 |
+ |
ABCB4 |
NNNGATCCAACAGTACGCTG |
NGG |
0 |
1.0 |
Tier II |
| 3 |
NC_000003.12 |
3 |
54376328 |
- |
CACNA2D3 |
NNNGATGCAACAGTAAGCTG |
NGG |
2 |
0.4706 |
Tier II |
| 4 |
NC_000002.12 |
2 |
44667195 |
+ |
CAMKMT |
NNNGATCCAACAGTTCCCTG |
NGG |
2 |
0.0471 |
Tier II |
| 5 |
NC_000002.12 |
2 |
31381368 |
+ |
XDH |
NNNGATCCAACAGCAGGCTG |
NGG |
2 |
0.044 |
Tier II |
| 6 |
NC_000004.12 |
4 |
120039304 |
+ |
LOC124900770 |
NNNGCTCCAACAGTACTCTG |
NGG |
2 |
0.125 |
Tier III |
| 7 |
NC_000002.12 |
2 |
139439080 |
+ |
LOC105373645 |
NNNGATCCAACAGTAAGCTG |
NGA |
2 |
0.0694 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
8976072 |
- |
Crot |
NNNGATCCATCAGTACGCTG |
NGG |
1 |
0.8824 |
Tier I |
| 2 |
NC_000067.6 |
1 |
181618368 |
+ |
Dnah14 |
NNNCACCCAACAGTACGCTG |
NGG |
2 |
0.4813 |
Tier II |
| 3 |
NC_000077.6 |
11 |
79040832 |
+ |
Ksr1 |
NNNGACCCAACAGTACCCTG |
NGG |
2 |
0.2139 |
Tier II |
| 4 |
NC_000075.6 |
9 |
108637148 |
+ |
Arih2 |
NNNGATCAAACAGTAGGCTG |
NGG |
2 |
0.1 |
Tier II |
| 5 |
NC_000072.6 |
6 |
127696409 |
- |
Prmt8 |
NNNGATCCAAGAGTACCCTG |
NGG |
2 |
0.0588 |
Tier II |
Other clones with same target sequence:
(none)
