Construct: sgRNA BRDN0001147222
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACCAGCAGAAGGTTAACCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LATS2 (26524)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77900
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000013.11 |
13 |
20987914 |
- |
LATS2 |
NNNAGCAGAAGGTTAACCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
97570209 |
- |
UBTD1 |
NNNAGCAGCAGGTTCACCGG |
NGG |
2 |
0.1558 |
Tier I |
| 3 |
NC_000019.10 |
19 |
43555453 |
- |
XRCC1 |
NNNAGCAGAAGCTTAACTGG |
NGG |
2 |
0.3403 |
Tier II |
| 4 |
NC_000010.11 |
10 |
17410774 |
- |
ST8SIA6 |
NNNAGCTGAAGGTTAACCAG |
NGG |
2 |
0.3125 |
Tier II |
| 5 |
NC_000012.12 |
12 |
71476056 |
+ |
LGR5 |
NNNAGCAGAAGGTTAAATGG |
NGG |
2 |
0.3 |
Tier II |
| 6 |
NC_000016.10 |
16 |
67230971 |
+ |
FHOD1 |
NNNAGCAGAAGGTTTACTGG |
NGG |
2 |
0.1286 |
Tier II |
| 7 |
NC_000001.11 |
1 |
230714080 |
- |
AGT |
NNNAGCAGAAGGTAAGCCGG |
NGG |
2 |
0.119 |
Tier II |
| 8 |
NC_000007.14 |
7 |
100951445 |
+ |
MUC3A |
NNNAGCAGAAGGTTAATGGG |
NGG |
2 |
0.0622 |
Tier II |
| 9 |
NC_000016.10 |
16 |
14572156 |
+ |
PARN |
NNNAGCAGAAGGTTAAGCGA |
NGG |
2 |
0.0551 |
Tier II |
| 10 |
NC_000015.10 |
15 |
47732337 |
- |
SEMA6D |
NNNAGCAGAAGGTTAACAGG |
NTG |
2 |
0.021 |
Tier II |
| 11 |
NC_000020.11 |
20 |
42789963 |
- |
PTPRT |
NNNAGCAGAAGATGAACCGG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000016.10 |
16 |
81605612 |
- |
CMIP |
NNNAGCAGAAGGTGAAACGG |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000006.12 |
6 |
29215729 |
- |
OR2H4P |
NNNAGCAGAAGGGTAACCGG |
NGG |
1 |
0.2609 |
Tier III |
| 14 |
NC_000007.14 |
7 |
100951445 |
+ |
LOC105375431 |
NNNAGCAGAAGGTTAATGGG |
NGG |
2 |
0.0622 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
57699291 |
- |
Lats2 |
NNNAGCAGAAGGTCAGCCGG |
NGG |
2 |
0.0549 |
Tier I |
| 2 |
NC_000071.6 |
5 |
150212412 |
- |
Fry |
NNNAGCAGCAGATTAACCGG |
NGG |
2 |
0.5333 |
Tier II |
| 3 |
NC_000080.6 |
14 |
41068587 |
+ |
Dydc2 |
NNNAGGAGAAGGTTAACTGG |
NGG |
2 |
0.3214 |
Tier II |
| 4 |
NC_000074.6 |
8 |
9226928 |
+ |
Fam155a |
NNNAGCAGAAGGTTCACCGG |
NGG |
1 |
0.2727 |
Tier II |
| 5 |
NC_000071.6 |
5 |
131842650 |
- |
Auts2 |
NNNAGCAGAAGGGTAACAGG |
NGG |
2 |
0.1405 |
Tier II |
| 6 |
NC_000076.6 |
10 |
33300231 |
- |
Trdn |
NNNAGCAGAAGGTTAAGCAG |
NGG |
2 |
0.042 |
Tier II |
| 7 |
NC_000086.7 |
X |
55989434 |
+ |
3830403N18Rik |
NNNAGCAGAAGGTTAAGCAG |
NGG |
2 |
0.042 |
Tier II |
| 8 |
NC_000068.7 |
2 |
164907358 |
- |
Zfp335 |
NNNAGCAGAAGGTTAAGGGG |
NGG |
2 |
0.0078 |
Tier II |
| 9 |
NC_000073.6 |
7 |
66688351 |
- |
Asb7 |
NNNAGCAGAAGGTGAACAGG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000079.6 |
13 |
62383476 |
- |
Gm3604 |
NNNAGGAGAAGGTGAACCGG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000076.6 |
10 |
33300231 |
- |
Gm19073 |
NNNAGCAGAAGGTTAAGCAG |
NGG |
2 |
0.042 |
Tier III |
Other clones with same target sequence:
(none)
