Construct: sgRNA BRDN0001147225

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCTCTGACCATCAGAAAGGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTK7 (5754)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78023

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 43130265 - PTK7 NNNCTGACCATCAGAAAGGG NGG 0 1.0 Tier I
2 NC_000016.10 16 6209702 + RBFOX1 NNNCTGATCTTCAGAAAGGG NGG 2 0.7721 Tier II
3 NC_000001.11 1 111571380 - RAP1A NNNCTGAACTTCAGAAAGGG NGG 2 0.5735 Tier II
4 NC_000010.11 10 11206907 - CELF2 NNNCTGAGCATCAGAAAGGT NGG 2 0.45 Tier II
5 NC_000005.10 5 5316824 + ADAMTS16 NNNCGGACCACCAGAAAGGG NGG 2 0.4267 Tier II
6 NC_000012.12 12 124141463 - ZNF664-RFLNA NNNATGACCATCAGAAAGGC NGG 2 0.3609 Tier II
7 NC_000007.14 7 132787187 + CHCHD3 NNNCTGACCAAGAGAAAGGG NGG 2 0.3333 Tier II
8 NC_000013.11 13 35461261 - NBEA NNNCTGACCATAACAAAGGG NGG 2 0.3061 Tier II
9 NC_000009.12 9 127837465 - ENG NNNCTGACCACCAGAAAGGC NGG 2 0.2857 Tier II
10 NC_000010.11 10 18010677 + SLC39A12 NNNCTGACCATCAGAAATGC NGG 2 0.2857 Tier II
11 NC_000005.10 5 7797042 - ADCY2 NNNCTCACCAGCAGAAAGGG NGG 2 0.2727 Tier II
12 NC_000023.11 X 155005646 + F8 NNNCTGAGCAGCAGAAAGGG NGG 2 0.2571 Tier II
13 NC_000001.11 1 84209428 - PRKACB NNNCTGACCAACTGAAAGGG NGG 2 0.225 Tier II
14 NC_000002.12 2 120969601 - GLI2 NNNCTGACCCTCAGAAATGG NGG 2 0.2222 Tier II
15 NC_000005.10 5 176493638 - FAF2 NNNCTGACCATATGAAAGGG NGG 2 0.2143 Tier II
16 NC_000004.12 4 142154119 - INPP4B NNNCTCACCATCAGAAAGGG NAG 2 0.1768 Tier II
17 NC_000003.12 3 141354609 - ZBTB38 NNNCTGACCATGAGCAAGGG NGG 2 0.1212 Tier II
18 NC_000001.11 1 27555727 - AHDC1 NNNCTGACCCTCAGTAAGGG NGG 2 0.0667 Tier II
19 NC_000001.11 1 218399590 - TGFB2 NNNCTGACCATCAGAAAAGG NGA 2 0.0481 Tier II
20 NC_000008.11 8 143735021 - IQANK1 NNNATGACCATCAGAAAGGG NTG 2 0.0328 Tier II
21 NC_000006.12 6 34523659 - PACSIN1 NNNCTCACCATCAGAAAGGG NTG 2 0.0266 Tier II
22 NC_000012.12 12 123050191 - PITPNM2 NNNCTGCCCATCAGAAAGGG NTG 2 0.017 Tier II
23 NC_000001.11 1 14784383 + KAZN NNNCTGACCATCAGAGAGGG NGT 2 0.0031 Tier II
24 NC_000003.12 3 107327797 - CSP2 NNNCTGACTATCAGAAAGTG NGG 2 0.5833 Tier III
25 NC_000010.11 10 11206907 - LOC124902375 NNNCTGAGCATCAGAAAGGT NGG 2 0.45 Tier III
26 NC_000002.12 2 12335807 - MIR3681HG NNNCTGAGCATCAGAAAAGG NGG 2 0.4451 Tier III
27 NC_000005.10 5 5316824 + LOC101929200 NNNCGGACCACCAGAAAGGG NGG 2 0.4267 Tier III
28 NC_000023.11 X 38974030 - LOC124905177 NNNCTGACCCTCAGAAAGGG NGG 1 0.3333 Tier III
29 NC_000006.12 6 74434765 - LOC101928516 NNNCAGAGCATCAGAAAGGG NGG 2 0.3214 Tier III
30 NC_000006.12 6 44001044 - LINC03040 NNNCTGACCATCAGTAAGGT NGG 2 0.14 Tier III
31 NC_000006.12 6 44001044 - SCIRT NNNCTGACCATCAGTAAGGT NGG 2 0.14 Tier III
32 NC_000016.10 16 20597269 + LOC112267906 NNNCTGACCATCAGAAAGTG NGA 2 0.0463 Tier III
33 NC_000001.11 1 190661637 + LINC01720 NNNCTGACCATCAGAAATGG NGA 2 0.0463 Tier III
34 NC_000013.11 13 50265088 - DLEU1 NNNCTGACCATCAGAAAAGG NGC 2 0.0154 Tier III
35 NC_000017.11 17 34726611 - LOC105371740 NNNCTGACCATCAGACAGGG NGG 1 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 46590417 + Ptk7 NNNCTGATCATCAGAGAGGG NGG 2 0.1683 Tier I
2 NC_000085.6 19 36563152 - Hectd2 NNNCTGACCATCAGAAAGTA NGG 2 0.625 Tier II
3 NC_000069.6 3 151803105 - Ptgfr NNNCTGATCATCAGAAAGGT NGG 2 0.6125 Tier II
4 NC_000068.7 2 130278053 + Nop56 NNNCTGACCTTCAGAAATGG NGG 2 0.5882 Tier II
5 NC_000082.6 16 44401112 + Cfap44 NNNCTGACCAAAAGAAAGGG NGG 2 0.5357 Tier II
6 NC_000086.7 X 83302113 - Dmd NNNCTGACCACCAAAAAGGG NGG 2 0.5 Tier II
7 NC_000073.6 7 144317343 + Shank2 NNNCTGACCACCAGAAAGTG NGG 2 0.4444 Tier II
8 NC_000074.6 8 48424297 - Tenm3 NNNCTGACCATGAGAAAGGA NGG 2 0.4167 Tier II
9 NC_000076.6 10 12650909 + Utrn NNNCTGACAATGAGAAAGGG NGG 2 0.381 Tier II
10 NC_000077.6 11 113373282 - Slc39a11 NNNCTGACCAGCAGAAAGGA NGG 2 0.375 Tier II
11 NC_000081.6 15 59419781 - Nsmce2 NNNCAGACCATCAGAAATGG NGG 2 0.3333 Tier II
12 NC_000067.6 1 90058056 - Iqca NNNCAGACCATCAGGAAGGG NGG 2 0.325 Tier II
13 NC_000085.6 19 57302532 - Ablim1 NNNCTGACCATCACAAAAGG NGG 2 0.2967 Tier II
14 NC_000068.7 2 172465417 - Rtf2 NNNCAGACCATCAGAAAGGC NGG 2 0.2143 Tier II
15 NC_000075.6 9 107668010 + Slc38a3 NNNCTGCCCATCAGAAACGG NGG 2 0.2083 Tier II
16 NC_000069.6 3 30409316 - Mecom NNNCTGACCATCAGTAAGTG NGG 2 0.1333 Tier II
17 NC_000070.6 4 151118559 - Camta1 NNNCTGACCATCAGAGAGGC NGG 2 0.0824 Tier II
18 NC_000084.6 18 76120354 + Zbtb7c NNNCTGACCATCTGAAAGGG NGC 2 0.0067 Tier II
19 NC_000080.6 14 96198118 + Klhl1 NNNCTGACCATCAGCAAGGG NGT 2 0.0044 Tier II
20 NC_000075.6 9 69905240 - Gm39368 NNNCTAGCCATCAGAAAGGG NGG 2 0.7059 Tier III
21 NC_000068.7 2 130278053 + Snord57 NNNCTGACCTTCAGAAATGG NGG 2 0.5882 Tier III
22 NC_000072.6 6 41257727 - Tcrb NNNCTGACAATCTGAAAGGG NGG 2 0.2571 Tier III
23 NC_000074.6 8 77766116 + LOC108167319 NNNCTGACCATCAACAAGGG NGG 2 0.2045 Tier III
24 NC_000076.6 10 24448241 + Gm15270 NNNCAGACCATCTGAAAGGG NGG 2 0.15 Tier III
25 NC_000072.6 6 96060754 - 2900060L22Rik NNNCTGTCCATCAGACAGGG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)