Construct: sgRNA BRDN0001147234
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATCCGGACCCTGGACAACGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- HCK (3055)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77214
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000020.11 |
20 |
32083976 |
+ |
HCK |
NNNCGGACCCTGGACAACGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
4241836 |
+ |
SDK1 |
NNNGTGACCCTGGACAACGG |
NGG |
2 |
0.15 |
Tier I |
| 3 |
NC_000008.11 |
8 |
91958304 |
+ |
RUNX1T1 |
NNNTGGACCCTGGACAACGA |
NGG |
2 |
0.75 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
136985027 |
- |
Znhit1 |
NNNAGGACCCTGGACAGCGG |
NGG |
2 |
0.1486 |
Tier I |
| 2 |
NC_000070.6 |
4 |
13891786 |
- |
Runx1t1 |
NNNTGGACCCTGGACAACGA |
NGG |
2 |
0.75 |
Tier II |
| 3 |
NC_000070.6 |
4 |
154021270 |
+ |
Smim1 |
NNNCAGACCCTGGACAACGC |
NGG |
2 |
0.3714 |
Tier II |
| 4 |
NC_000070.6 |
4 |
154021270 |
+ |
Lrrc47 |
NNNCAGACCCTGGACAACGC |
NGG |
2 |
0.3714 |
Tier II |
| 5 |
NC_000073.6 |
7 |
30500888 |
- |
Prodh2 |
NNNCGGACCCTGGACAACTG |
NAG |
2 |
0.1728 |
Tier II |
| 6 |
NC_000076.6 |
10 |
127666551 |
- |
Nab2 |
NNNCGGAGCCTGGACAGCGG |
NGG |
2 |
0.1134 |
Tier II |
| 7 |
NC_000085.6 |
19 |
42752568 |
- |
Pyroxd2 |
NNNCGGACCCTGGGAAACGG |
NGG |
2 |
0.1037 |
Tier II |
| 8 |
NC_000074.6 |
8 |
119558687 |
+ |
Mbtps1 |
NNNCGGACCCGGGAGAACGG |
NGG |
2 |
0.02 |
Tier II |
| 9 |
NC_000073.6 |
7 |
137133251 |
- |
Gm39109 |
NNNCTGACCCTGAACAACGG |
NGG |
2 |
0.2769 |
Tier III |
Other clones with same target sequence:
(none)
