Construct: sgRNA BRDN0001147235
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACTGTCAACGAAACCTGGCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LIMK2 (3985)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75973
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000022.11 |
22 |
31225802 |
+ |
LIMK2 |
NNNGTCAACGAAACCTGGCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
68843780 |
- |
PIP5K1B |
NNNGTAAAAGAAACCTGGCA |
NGG |
2 |
0.7959 |
Tier II |
| 3 |
NC_000002.12 |
2 |
136791272 |
+ |
THSD7B |
NNNGGCAAGGAAACCTGGCA |
NGG |
2 |
0.3962 |
Tier II |
| 4 |
NC_000017.11 |
17 |
31489076 |
- |
RAB11FIP4 |
NNNGTCAAGGAAAACTGGCA |
NGG |
2 |
0.2167 |
Tier II |
| 5 |
NC_000012.12 |
12 |
31305202 |
+ |
SINHCAF |
NNNGTCAACGATACCTGGTA |
NGG |
2 |
0.1538 |
Tier II |
| 6 |
NC_000006.12 |
6 |
166309481 |
+ |
LOC107986669 |
NNNGTCAAGGAAACCTGGCA |
NGT |
2 |
0.01 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
4379232 |
+ |
Hormad2 |
NNNGTCAACTAAACCAGGCA |
NGG |
2 |
0.7386 |
Tier II |
| 2 |
NC_000068.7 |
2 |
153677685 |
+ |
Dnmt3b |
NNNGTCAGGGAAACCTGGCA |
NGG |
2 |
0.454 |
Tier II |
| 3 |
NC_000081.6 |
15 |
84326806 |
- |
Parvg |
NNNGTCAACCAAACCTTGCA |
NGG |
2 |
0.1 |
Tier II |
| 4 |
NC_000080.6 |
14 |
78946017 |
- |
Vwa8 |
NNNGTCAACAAAACGTGGCA |
NGG |
2 |
0.0467 |
Tier II |
| 5 |
NC_000076.6 |
10 |
73268763 |
+ |
Pcdh15 |
NNNGTCAACGAAACCTTGCA |
NTG |
2 |
0.0097 |
Tier II |
Other clones with same target sequence:
(none)
