Construct: sgRNA BRDN0001147236
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTCTTGAGTACTTGCACTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- AKT2 (208)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77504
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
40237999 |
- |
AKT2 |
NNNTTGAGTACTTGCACTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
11231358 |
- |
HRH1 |
NNNTTGAGTAATTGCACTCG |
NGG |
1 |
0.75 |
Tier II |
| 3 |
NC_000009.12 |
9 |
132472271 |
+ |
CFAP77 |
NNNTTGAGTACTGGCACTCA |
NGG |
2 |
0.2446 |
Tier II |
| 4 |
NC_000003.12 |
3 |
139494818 |
- |
ACTG1P1 |
NNNCTGAGTACTTGCACTCT |
NGG |
2 |
0.4529 |
Tier III |
| 5 |
NC_000003.12 |
3 |
139494818 |
- |
COPB2-DT |
NNNCTGAGTACTTGCACTCT |
NGG |
2 |
0.4529 |
Tier III |
| 6 |
NC_000005.10 |
5 |
15451701 |
- |
LOC391741 |
NNNCTGAGTACTTGCACTCT |
NGG |
2 |
0.4529 |
Tier III |
| 7 |
NC_000005.10 |
5 |
15451701 |
- |
LOC124900945 |
NNNCTGAGTACTTGCACTCT |
NGG |
2 |
0.4529 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
63347871 |
- |
Fbxl17 |
NNNCTGAGTACTTGCACTCA |
NGG |
2 |
0.6066 |
Tier II |
| 2 |
NC_000068.7 |
2 |
59733208 |
- |
Tanc1 |
NNNTTGAGTAATTGCACTCT |
NGG |
2 |
0.525 |
Tier II |
| 3 |
NC_000075.6 |
9 |
67888906 |
+ |
Vps13c |
NNNTTGAGTGCTTGCACTCA |
NGG |
2 |
0.5208 |
Tier II |
| 4 |
NC_000079.6 |
13 |
100155064 |
- |
Naip2 |
NNNTTGAGAACTTGCACTTG |
NGG |
2 |
0.4286 |
Tier II |
| 5 |
NC_000079.6 |
13 |
100219873 |
- |
Naip5 |
NNNTTGAGAACTTGCACTTG |
NGG |
2 |
0.4286 |
Tier II |
| 6 |
NC_000079.6 |
13 |
100423262 |
- |
Naip1 |
NNNTTGAGAACTTGCACTTG |
NGG |
2 |
0.4286 |
Tier II |
| 7 |
NC_000071.6 |
5 |
122777004 |
- |
Camkk2 |
NNNTTGAGTCCTTGGACTCG |
NGG |
2 |
0.0167 |
Tier II |
| 8 |
NC_000076.6 |
10 |
93489477 |
+ |
Hal |
NNNTTGAGTACTTGCCCTGG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000083.6 |
17 |
63347871 |
- |
Gm18418 |
NNNCTGAGTACTTGCACTCA |
NGG |
2 |
0.6066 |
Tier III |
| 10 |
NC_000079.6 |
13 |
100271132 |
- |
Naip3-ps1 |
NNNTTGAGAACTTGCACTTG |
NGG |
2 |
0.4286 |
Tier III |
| 11 |
NC_000079.6 |
13 |
100349157 |
- |
Naip3 |
NNNTTGAGAACTTGCACTTG |
NGG |
2 |
0.4286 |
Tier III |
| 12 |
NC_000077.6 |
11 |
114234253 |
- |
Gm11690 |
NNNTTGAGTAATTGCACTTG |
NGG |
2 |
0.3462 |
Tier III |
| 13 |
NC_000085.6 |
19 |
16009187 |
- |
C130060C02Rik |
NNNTTGAGTTCTTGCACTGG |
NGG |
2 |
0.1103 |
Tier III |
Other clones with same target sequence:
(none)
