Construct: sgRNA BRDN0001147240

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACTAAATGAAGTCTTCATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NADK2 (133686)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78092

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 36211866 - NADK2 NNNAAATGAAGTCTTCATTG NGG 0 1.0 Tier I
2 NC_000001.11 1 91261317 - HFM1 NNNAAATGAAGTCTTTATTG NGA 2 0.0214 Tier I
3 NC_000008.11 8 73269071 + C8orf89 NNNAAATGTAGACTTCATTG NGG 2 0.48 Tier II
4 NC_000012.12 12 32590985 - FGD4 NNNAACTGAAGCCTTCATTG NGG 2 0.4306 Tier II
5 NC_000010.11 10 127415475 - DOCK1 NNNAAATGAAATCTTCATTC NGG 2 0.4286 Tier II
6 NC_000010.11 10 132273061 - STK32C NNNAATTGCAGTCTTCATTG NGG 2 0.4082 Tier II
7 NC_000010.11 10 34405966 - PARD3 NNNAACAGAAGTCTTCATTG NGG 2 0.3977 Tier II
8 NC_000001.11 1 209603406 + CAMK1G NNNAAATGAAGACTTCATTC NGG 2 0.3429 Tier II
9 NC_000004.12 4 150544511 - LRBA NNNAAAGGAAGGCTTCATTG NGG 2 0.2941 Tier II
10 NC_000007.14 7 18686035 + HDAC9 NNNAAATGAAGACTTCAGTG NGG 2 0.2667 Tier II
11 NC_000009.12 9 3446316 + RFX3 NNNAAATGAAGTCTTCATTG NAG 1 0.2593 Tier II
12 NC_000002.12 2 85864818 - ST3GAL5 NNNAAATGTAGTCTTCATTC NGG 2 0.2571 Tier II
13 NC_000014.9 14 23076308 - ACIN1 NNNAAATGCAGTCTTCACTG NGG 2 0.2449 Tier II
14 NC_000011.10 11 4943992 + MMP26 NNNAAATGAAGTCTTCATAT NGG 2 0.2 Tier II
15 NC_000011.10 11 4943992 + OR51A4 NNNAAATGAAGTCTTCATAT NGG 2 0.2 Tier II
16 NC_000008.11 8 51819953 + PCMTD1 NNNAAATGAAGTTTTCATAG NGG 2 0.2 Tier II
17 NC_000012.12 12 61718038 - TAFA2 NNNAAATGAAGTCTTCAAAG NGG 2 0.1905 Tier II
18 NC_000016.10 16 7382192 - RBFOX1 NNNAAATGGAGTCTTCATAG NGG 2 0.1905 Tier II
19 NC_000002.12 2 230787503 + CAB39 NNNAATTGAAGTCTTCATTG NAG 2 0.1852 Tier II
20 NC_000001.11 1 177132382 + ASTN1 NNNAAATGAAGTCATCATAG NGG 2 0.1769 Tier II
21 NC_000004.12 4 118713805 - METTL14 NNNAAATGAAGTCTTCAATG NAG 2 0.1728 Tier II
22 NC_000004.12 4 796334 - CPLX1 NNNACATGAAGTCTTCAGTG NGG 2 0.1667 Tier II
23 NC_000015.10 15 52622435 - ATOSA NNNAAATGAAGTCTACATAG NGG 2 0.1654 Tier II
24 NC_000015.10 15 57283896 - TCF12 NNNAAATGAAATCTTCTTTG NGG 2 0.1333 Tier II
25 NC_000004.12 4 153177335 + TRIM2 NNNAAATGAAGTCTTTATTC NGG 2 0.1319 Tier II
26 NC_000003.12 3 189743554 + TP63 NNNAAATGAAGGCTTCATTG NAG 2 0.1296 Tier II
27 NC_000006.12 6 55484440 - HMGCLL1 NNNATATGAAGTCTTCAGTG NGG 2 0.1212 Tier II
28 NC_000004.12 4 62056639 + ADGRL3 NNNAAATGAAGTCTCCACTG NGG 2 0.1169 Tier II
29 NC_000002.12 2 50450507 + NRXN1 NNNAAATGAAGACTTCTTTG NGG 2 0.1067 Tier II
30 NC_000002.12 2 209831073 + UNC80 NNNAAATGAATTCTCCATTG NGG 2 0.1049 Tier II
31 NC_000006.12 6 114336754 - HS3ST5 NNNAAATGAAGTATTCATTG NAG 2 0.0997 Tier II
32 NC_000020.11 20 38829533 + PPP1R16B NNNAAATGAGGTCTTCCTTG NGG 2 0.098 Tier II
33 NC_000020.11 20 38831221 + PPP1R16B NNNAAATGAGGTCTTCCTTG NGG 2 0.098 Tier II
34 NC_000008.11 8 118201472 - SAMD12 NNNAATTGAAGTGTTCATTG NGG 2 0.0974 Tier II
35 NC_000020.11 20 6779200 - BMP2 NNNAAATAAAGTCTTCATTG NGA 2 0.0694 Tier II
36 NC_000003.12 3 94001470 - ARL13B NNNAAATGAAGACTTCATTG NGA 2 0.0556 Tier II
37 NC_000009.12 9 124471882 + ADGRD2 NNNAAATGAAGTGTTCAGTG NGG 2 0.0455 Tier II
38 NC_000006.12 6 117685132 + NUS1 NNNAAATGAAGTCTTCATTT NGC 2 0.0156 Tier II
39 NC_000003.12 3 42845008 - ACKR2 NNNAAATGAAGTCTTCAATG NGC 2 0.0148 Tier II
40 NC_000023.11 X 17132072 - REPS2 NNNAAATGAAGACTTCATTG NGT 2 0.0129 Tier II
41 NC_000001.11 1 114509812 + TRIM33 NNNGAATGAAGTCTTCATTG NGT 2 0.0101 Tier II
42 NC_000002.12 2 214130409 - SPAG16 NNNAAATGAAGTCTTCATGG NTG 2 0.0097 Tier II
43 NC_000011.10 11 125405378 + PKNOX2 NNNAAATGAAGTCTTCCTTG NGT 2 0.0028 Tier II
44 NC_000012.12 12 80663937 - PTPRQ NNNAAATGAAGTCTTGATTG NGT 2 0.0025 Tier II
45 NC_000003.12 3 61303729 + LOC105377114 NNNAAATGAAATCATCATTG NGG 2 0.619 Tier III
46 NC_000003.12 3 61303729 + LOC105377115 NNNAAATGAAATCATCATTG NGG 2 0.619 Tier III
47 NC_000001.11 1 189499409 + LOC105371657 NNNAAATTAAGACTTCATTG NGG 2 0.5 Tier III
48 NC_000005.10 5 131024535 + LOC105379172 NNNAAATGAAGTCTTCATTC NGG 1 0.4286 Tier III
49 NC_000002.12 2 113997845 - LINC01191 NNNAAATGAAGGCTTCATTT NGG 2 0.35 Tier III
50 NC_000005.10 5 54681636 + LINC02998 NNNAACTGAAGTTTTCATTG NGG 2 0.3182 Tier III
51 NC_000013.11 13 81553358 - LOC105370283 NNNCAATGATGTCTTCATTG NGG 2 0.3114 Tier III
52 NC_000023.11 X 128582252 + LOC107985698 NNNGAATGAAGTCTTCATTC NGG 2 0.2679 Tier III
53 NC_000001.11 1 203271251 + LOC124904486 NNNAAATGCAGTCTTCATTC NGG 2 0.2449 Tier III
54 NC_000012.12 12 69717917 + LOC101928002 NNNGTATGAAGTCTTCATTG NGG 2 0.2273 Tier III
55 NC_000004.12 4 796334 - LOC105374339 NNNACATGAAGTCTTCAGTG NGG 2 0.1667 Tier III
56 NC_000018.10 18 42322316 + LINC00907 NNNAGATGAAGTCTTCCTTG NGG 2 0.1271 Tier III
57 NC_000015.10 15 51923739 - LOC100422556 NNNAAATGAAGTCTACCTTG NGG 2 0.1022 Tier III
58 NC_000015.10 15 51923739 - LOC112268148 NNNAAATGAAGTCTACCTTG NGG 2 0.1022 Tier III
59 NC_000004.12 4 113984894 + LOC124900762 NNNCAATGAAGTCCTCATTG NGG 2 0.1008 Tier III
60 NC_000006.12 6 114336754 - HDAC2-AS2 NNNAAATGAAGTATTCATTG NAG 2 0.0997 Tier III
61 NC_000012.12 12 126697242 + LINC02824 NNNAAATGAAGTCTTCAGGG NGG 2 0.0833 Tier III
62 NC_000011.10 11 78154701 - KCTD21-AS1 NNNAACTGAAGTCTTCCTTG NGG 2 0.0802 Tier III
63 NC_000016.10 16 60484950 + LOC101927605 NNNAAAAGAAGTCTGCATTG NGG 2 0.0438 Tier III
64 NC_000011.10 11 28004468 + LOC124902655 NNNAAATGTAGTCTTCATTG NGA 2 0.0417 Tier III
65 NC_000008.11 8 24431023 + ADAM7-AS1 NNNAAATGAAGTCTTCATTG NGC 1 0.0222 Tier III
66 NC_000003.12 3 42845008 - LOC124909373 NNNAAATGAAGTCTTCAATG NGC 2 0.0148 Tier III
67 NC_000023.11 X 153051496 - HMGN2P48 NNNAGATGAAGTCTTCATTG NGT 2 0.0116 Tier III
68 NC_000012.12 12 80663937 - LOC105369867 NNNAAATGAAGTCTTGATTG NGT 2 0.0025 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 9096071 + Nadk2 NNNCAATGAAGTCTTCATTG NGG 1 0.3529 Tier I
2 NC_000067.6 1 181182798 + Wdr26 NNNCAATGAAGTCTTCATTA NGG 2 0.3309 Tier I
3 NC_000068.7 2 113491996 - Fmn1 NNNAAATGAAGTCTTAATTG NGG 1 1.0 Tier II
4 NC_000068.7 2 17639562 + Nebl NNNAAACGAAATCTTCATTG NGG 2 0.6875 Tier II
5 NC_000082.6 16 72438730 + Robo1 NNNAGATGAAGCCTTCATTG NGG 2 0.6821 Tier II
6 NC_000075.6 9 66957287 + Lactb NNNAGATGAAGTCTTCATTA NGG 2 0.675 Tier II
7 NC_000074.6 8 111541779 - Znrf1 NNNAAATCAAGTCTTCATTA NGG 2 0.5769 Tier II
8 NC_000077.6 11 32572935 + Stk10 NNNAGATGAAGACTTCATTG NGG 2 0.576 Tier II
9 NC_000082.6 16 41203403 - Lsamp NNNAAATGAAGATTTCATTG NGG 2 0.56 Tier II
10 NC_000073.6 7 64213951 + Trpm1 NNNTGATGAAGTCTTCATTG NGG 2 0.4582 Tier II
11 NC_000072.6 6 34307242 - Akr1b3 NNNAAATTAAGTCTTCATTT NGG 2 0.4375 Tier II
12 NC_000073.6 7 35537225 - Tdrd12 NNNAAATGAAATATTCATTG NGG 2 0.3846 Tier II
13 NC_000076.6 10 60732695 - Slc29a3 NNNAAAGGCAGTCTTCATTG NGG 2 0.3361 Tier II
14 NC_000071.6 5 109347011 + Vmn2r16 NNNACATGGAGTCTTCATTG NGG 2 0.3333 Tier II
15 NC_000085.6 19 19061834 + Rorb NNNAAATGAACTCTTCATTT NGG 2 0.3 Tier II
16 NC_000081.6 15 58573153 + Fer1l6 NNNAACTTAAGTCTTCATTG NGG 2 0.2841 Tier II
17 NC_000079.6 13 109205991 - Pde4d NNNAAATGAAGTCTCCATTG NGG 1 0.2727 Tier II
18 NC_000083.6 17 91013503 - Nrxn1 NNNAAATGAAGCCTTCATTG NAG 2 0.2456 Tier II
19 NC_000069.6 3 68956184 + Ift80 NNNAACTGAAGTCTTCACTG NGG 2 0.1948 Tier II
20 NC_000067.6 1 93220552 - Crocc2 NNNAATTGAAGTCTTCATTG NAG 2 0.1852 Tier II
21 NC_000069.6 3 141774243 + Unc5c NNNAAATGAAGTCTTCATTT NAG 2 0.1815 Tier II
22 NC_000071.6 5 147546949 - Pan3 NNNAGATGAAGTCTTCATGG NGG 2 0.18 Tier II
23 NC_000072.6 6 56135150 - Pde1c NNNAAAGGAAGTCTTCATGG NGG 2 0.1471 Tier II
24 NC_000082.6 16 6619572 - Rbfox1 NNNAAAAGAAGTGTTCATTG NGG 2 0.1193 Tier II
25 NC_000068.7 2 133561822 - Bmp2 NNNAAATAAAGTCTTCATTG NGA 2 0.0694 Tier II
26 NC_000075.6 9 48379203 - Nxpe4 NNNAAATGAAGTCTGAATTG NGG 2 0.05 Tier II
27 NC_000069.6 3 24395297 + Naaladl2 NNNAAATGAAGTCTTCATTT NGA 2 0.0486 Tier II
28 NC_000080.6 14 48476629 - Tmem260 NNNAAATGAAGTGTTCATTG NAG 2 0.0354 Tier II
29 NC_000073.6 7 87596322 - Grm5 NNNAAATCAAGTCTTCATTG NGT 2 0.0099 Tier II
30 NC_000074.6 8 90940383 - Chd9 NNNAAATGAAGTGTTCATTG NGA 2 0.0095 Tier II
31 NC_000071.6 5 125740574 + Tmem132b NNNAAATGAAGTTGTCATTG NGG 2 0.0 Tier II
32 NC_000078.6 12 113361564 - Igh NNNAAATGAAGGCTTCATTG NGG 1 0.5 Tier III
33 NC_000069.6 3 94295472 - Gm36010 NNNAATTGAAGTCTTCATTG NAG 2 0.1852 Tier III
34 NC_000083.6 17 52154685 - Gm19585 NNNCTATGAAGTCTTCATTG NGG 2 0.1283 Tier III
35 NC_000085.6 19 56665161 - Gm52389 NNNAAATGAAGCCTTCTTTG NGG 2 0.1263 Tier III
36 NC_000080.6 14 123095887 + Gm33906 NNNAAATGAAGTATTGATTG NGG 2 0.0592 Tier III
37 NC_000076.6 10 126960873 + Gm30539 NNNAAATGAAGTCTGCATTT NGG 2 0.035 Tier III
38 NC_000072.6 6 137857313 - Gm38552 NNNAAATGAAGTCTTTATTG NGA 2 0.0214 Tier III
39 NC_000077.6 11 110550535 + Gm46300 NNNAAATGATGTCTTCATTG NGT 2 0.0142 Tier III
Download CSV

Other clones with same target sequence:

(none)