Construct: sgRNA BRDN0001147242
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATGAAGTATGACGAGCATA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IP6K3 (117283)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77655
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
33735368 |
- |
IP6K3 |
NNNGAAGTATGACGAGCATA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
20643225 |
+ |
PDE3A |
NNNGAAGTATGATGAGAATA |
NGG |
2 |
0.3267 |
Tier II |
| 3 |
NC_000012.12 |
12 |
100052626 |
+ |
BLTP3B |
NNNGAAGTATAACGAGCACA |
NGG |
2 |
0.2759 |
Tier II |
| 4 |
NC_000006.12 |
6 |
109665812 |
- |
AK9 |
NNNGAAGTATTACCAGCATA |
NGG |
2 |
0.1648 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
23666044 |
+ |
Kcnma1 |
NNNGAGGTATGATGAGCATA |
NGG |
2 |
0.5 |
Tier II |
| 2 |
NC_000067.6 |
1 |
170196774 |
- |
Uhmk1 |
NNNGAAGTATGAGGGGCATA |
NGG |
2 |
0.0886 |
Tier II |
| 3 |
NC_000069.6 |
3 |
59065541 |
+ |
Med12l |
NNNAAAGTATGACGAGGATA |
NGG |
2 |
0.0529 |
Tier II |
| 4 |
NC_000072.6 |
6 |
126764858 |
- |
Gm20190 |
NNNGAAGTCTGACGAGCATA |
NGA |
2 |
0.0397 |
Tier III |
Other clones with same target sequence:
(none)
