Construct: sgRNA BRDN0001147242

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GATGAAGTATGACGAGCATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IP6K3 (117283)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77655

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 33735368 - IP6K3 NNNGAAGTATGACGAGCATA NGG 0 1.0 Tier I
2 NC_000012.12 12 20643225 + PDE3A NNNGAAGTATGATGAGAATA NGG 2 0.3267 Tier II
3 NC_000012.12 12 100052626 + BLTP3B NNNGAAGTATAACGAGCACA NGG 2 0.2759 Tier II
4 NC_000006.12 6 109665812 - AK9 NNNGAAGTATTACCAGCATA NGG 2 0.1648 Tier II
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 23666044 + Kcnma1 NNNGAGGTATGATGAGCATA NGG 2 0.5 Tier II
2 NC_000067.6 1 170196774 - Uhmk1 NNNGAAGTATGAGGGGCATA NGG 2 0.0886 Tier II
3 NC_000069.6 3 59065541 + Med12l NNNAAAGTATGACGAGGATA NGG 2 0.0529 Tier II
4 NC_000072.6 6 126764858 - Gm20190 NNNGAAGTCTGACGAGCATA NGA 2 0.0397 Tier III
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Other clones with same target sequence:

(none)