Construct: sgRNA BRDN0001147244

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAAGTGATTAGTAATGAAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PEAK1 (79834)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75854

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 77181265 - PEAK1 NNNGTGATTAGTAATGAAGG NGG 0 1.0 Tier I
2 NC_000017.11 17 69110897 + ABCA6 NNNGTGATGAGTAATGAAGG NTG 2 0.0241 Tier I
3 NC_000004.12 4 2903085 + ADD1 NNNGTGATTAGTAATGAAGA NGG 1 0.9375 Tier II
4 NC_000011.10 11 14229536 - SPON1 NNNGTGAATAGTAATGAAGG NGG 1 0.8 Tier II
5 NC_000008.11 8 47284887 + SPIDR NNNGTTATTAGTAATGAAGA NGG 2 0.625 Tier II
6 NC_000016.10 16 27467069 + GTF3C1 NNNGAGATCAGTAATGAAGG NGG 2 0.4615 Tier II
7 NC_000023.11 X 18572522 - CDKL5 NNNGTGATTAGAAATGATGG NGG 2 0.4 Tier II
8 NC_000002.12 2 132195258 + ANKRD30BL NNNGTGATTAGTGGTGAAGG NGG 2 0.3043 Tier II
9 NC_000023.11 X 16681256 + CTPS2 NNNGTGATTAGGAAAGAAGG NGG 2 0.2895 Tier II
10 NC_000005.10 5 78466857 + SCAMP1 NNNCTGATTAGTAATGATGG NGG 2 0.2647 Tier II
11 NC_000007.14 7 89092245 - ZNF804B NNNGTGATTAGTAATGAAGG NAG 1 0.2593 Tier II
12 NC_000009.12 9 125298177 + GAPVD1 NNNGTGATTAGCAATGGAGG NGG 2 0.1672 Tier II
13 NC_000014.9 14 36690692 + SLC25A21 NNNGTGATTAGTACTGAAGT NGG 2 0.15 Tier II
14 NC_000003.12 3 22341618 - ZNF385D NNNGTGAATAGTAATGGAGG NGG 2 0.1412 Tier II
15 NC_000008.11 8 10063613 + MSRA NNNGTGATTAGTCATGAGGG NGG 2 0.0842 Tier II
16 NC_000013.11 13 23826042 + MIPEP NNNGTGATTAGTAATGTAGC NGG 2 0.0571 Tier II
17 NC_000020.11 20 36119183 + EPB41L1 NNNGTGATTAGTACTGAAGG NAG 2 0.0556 Tier II
18 NC_000005.10 5 145491371 - PRELID2 NNNGTGATTAGTCATGAAGG NAG 2 0.0546 Tier II
19 NC_000006.12 6 72325894 - RIMS1 NNNGTGATTAATAATCAAGG NGG 2 0.0 Tier II
20 NC_000021.9 21 28175871 - LINC01695 NNNGTGATTAGAAATAAAGG NGG 2 0.8 Tier III
21 NC_000004.12 4 63487089 - LOC105377253 NNNGTCATTAGTAATGAATG NGG 2 0.4545 Tier III
22 NC_000008.11 8 10063613 + LOC124901885 NNNGTGATTAGTCATGAGGG NGG 2 0.0842 Tier III
23 NC_000020.11 20 18357525 - ZNF133-AS1 NNNGTGATTTGTAAGGAAGG NGG 2 0.0441 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 30619711 - Kcnk3 NNNCTGATTAATAATGAAGG NGG 2 0.5294 Tier II
2 NC_000072.6 6 80199969 + Lrrtm4 NNNGTGATTGGTAATGAAGA NGG 2 0.5208 Tier II
3 NC_000071.6 5 66854422 + Limch1 NNNGTGGTTAGTAATGAAAG NGG 2 0.5042 Tier II
4 NC_000076.6 10 96033630 - Eea1 NNNGTGAATAGTAAAGAAGG NGG 2 0.4632 Tier II
5 NC_000079.6 13 46889485 + Kif13a NNNGTGCTTAGAAATGAAGG NGG 2 0.35 Tier II
6 NC_000086.7 X 107677461 - Tbx22 NNNGTGATTTTTAATGAAGG NGG 2 0.3394 Tier II
7 NC_000072.6 6 46480076 + Cntnap2 NNNGTGATGAGGAATGAAGG NGG 2 0.3095 Tier II
8 NC_000082.6 16 45386968 + Cd200 NNNGTGATTAGTAATGAAGA NAG 2 0.2431 Tier II
9 NC_000072.6 6 27480615 - Grm8 NNNGTGATTACTAATGAAGC NGG 2 0.1837 Tier II
10 NC_000070.6 4 66052346 - Astn2 NNNGTGATTAGGTATGAAGG NGG 2 0.15 Tier II
11 NC_000070.6 4 85751903 - Adamtsl1 NNNGTGATTTGTAATGTAGG NGG 2 0.1176 Tier II
12 NC_000084.6 18 45477216 - Kcnn2 NNNGTGATAAGTAATGAAGG NTG 2 0.0362 Tier II
13 NC_000075.6 9 10449276 + Cntn5 NNNGTGCTTAGTAAGGAAGG NGG 2 0.0219 Tier II
14 NC_000082.6 16 66975977 + Cadm2 NNNGTGATTAGAAATGAAGG NGT 2 0.0129 Tier II
15 NC_000072.6 6 51280171 - Gm38821 NNNGTGATTAGTACTGAAGG NGT 2 0.0035 Tier II
16 NC_000078.6 12 26212671 - 4930480M12Rik NNNGAGATCAGTAATGAAGG NGG 2 0.4615 Tier III
17 NC_000070.6 4 11033356 - Gm35429 NNNGTTATTGGTAATGAAGG NGG 2 0.3704 Tier III
18 NC_000084.6 18 45477216 - Gm31907 NNNGTGATAAGTAATGAAGG NTG 2 0.0362 Tier III
19 NC_000073.6 7 78706267 - E430016F16Rik NNNGTGATTAGTAATGATGG NGT 2 0.0081 Tier III
Download CSV

Other clones with same target sequence:

(none)

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