Construct: sgRNA BRDN0001147244
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAAGTGATTAGTAATGAAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PEAK1 (79834)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75854
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
77181265 |
- |
PEAK1 |
NNNGTGATTAGTAATGAAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
69110897 |
+ |
ABCA6 |
NNNGTGATGAGTAATGAAGG |
NTG |
2 |
0.0241 |
Tier I |
| 3 |
NC_000004.12 |
4 |
2903085 |
+ |
ADD1 |
NNNGTGATTAGTAATGAAGA |
NGG |
1 |
0.9375 |
Tier II |
| 4 |
NC_000011.10 |
11 |
14229536 |
- |
SPON1 |
NNNGTGAATAGTAATGAAGG |
NGG |
1 |
0.8 |
Tier II |
| 5 |
NC_000008.11 |
8 |
47284887 |
+ |
SPIDR |
NNNGTTATTAGTAATGAAGA |
NGG |
2 |
0.625 |
Tier II |
| 6 |
NC_000016.10 |
16 |
27467069 |
+ |
GTF3C1 |
NNNGAGATCAGTAATGAAGG |
NGG |
2 |
0.4615 |
Tier II |
| 7 |
NC_000023.11 |
X |
18572522 |
- |
CDKL5 |
NNNGTGATTAGAAATGATGG |
NGG |
2 |
0.4 |
Tier II |
| 8 |
NC_000002.12 |
2 |
132195258 |
+ |
ANKRD30BL |
NNNGTGATTAGTGGTGAAGG |
NGG |
2 |
0.3043 |
Tier II |
| 9 |
NC_000023.11 |
X |
16681256 |
+ |
CTPS2 |
NNNGTGATTAGGAAAGAAGG |
NGG |
2 |
0.2895 |
Tier II |
| 10 |
NC_000005.10 |
5 |
78466857 |
+ |
SCAMP1 |
NNNCTGATTAGTAATGATGG |
NGG |
2 |
0.2647 |
Tier II |
| 11 |
NC_000007.14 |
7 |
89092245 |
- |
ZNF804B |
NNNGTGATTAGTAATGAAGG |
NAG |
1 |
0.2593 |
Tier II |
| 12 |
NC_000009.12 |
9 |
125298177 |
+ |
GAPVD1 |
NNNGTGATTAGCAATGGAGG |
NGG |
2 |
0.1672 |
Tier II |
| 13 |
NC_000014.9 |
14 |
36690692 |
+ |
SLC25A21 |
NNNGTGATTAGTACTGAAGT |
NGG |
2 |
0.15 |
Tier II |
| 14 |
NC_000003.12 |
3 |
22341618 |
- |
ZNF385D |
NNNGTGAATAGTAATGGAGG |
NGG |
2 |
0.1412 |
Tier II |
| 15 |
NC_000008.11 |
8 |
10063613 |
+ |
MSRA |
NNNGTGATTAGTCATGAGGG |
NGG |
2 |
0.0842 |
Tier II |
| 16 |
NC_000013.11 |
13 |
23826042 |
+ |
MIPEP |
NNNGTGATTAGTAATGTAGC |
NGG |
2 |
0.0571 |
Tier II |
| 17 |
NC_000020.11 |
20 |
36119183 |
+ |
EPB41L1 |
NNNGTGATTAGTACTGAAGG |
NAG |
2 |
0.0556 |
Tier II |
| 18 |
NC_000005.10 |
5 |
145491371 |
- |
PRELID2 |
NNNGTGATTAGTCATGAAGG |
NAG |
2 |
0.0546 |
Tier II |
| 19 |
NC_000006.12 |
6 |
72325894 |
- |
RIMS1 |
NNNGTGATTAATAATCAAGG |
NGG |
2 |
0.0 |
Tier II |
| 20 |
NC_000021.9 |
21 |
28175871 |
- |
LINC01695 |
NNNGTGATTAGAAATAAAGG |
NGG |
2 |
0.8 |
Tier III |
| 21 |
NC_000004.12 |
4 |
63487089 |
- |
LOC105377253 |
NNNGTCATTAGTAATGAATG |
NGG |
2 |
0.4545 |
Tier III |
| 22 |
NC_000008.11 |
8 |
10063613 |
+ |
LOC124901885 |
NNNGTGATTAGTCATGAGGG |
NGG |
2 |
0.0842 |
Tier III |
| 23 |
NC_000020.11 |
20 |
18357525 |
- |
ZNF133-AS1 |
NNNGTGATTTGTAAGGAAGG |
NGG |
2 |
0.0441 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
30619711 |
- |
Kcnk3 |
NNNCTGATTAATAATGAAGG |
NGG |
2 |
0.5294 |
Tier II |
| 2 |
NC_000072.6 |
6 |
80199969 |
+ |
Lrrtm4 |
NNNGTGATTGGTAATGAAGA |
NGG |
2 |
0.5208 |
Tier II |
| 3 |
NC_000071.6 |
5 |
66854422 |
+ |
Limch1 |
NNNGTGGTTAGTAATGAAAG |
NGG |
2 |
0.5042 |
Tier II |
| 4 |
NC_000076.6 |
10 |
96033630 |
- |
Eea1 |
NNNGTGAATAGTAAAGAAGG |
NGG |
2 |
0.4632 |
Tier II |
| 5 |
NC_000079.6 |
13 |
46889485 |
+ |
Kif13a |
NNNGTGCTTAGAAATGAAGG |
NGG |
2 |
0.35 |
Tier II |
| 6 |
NC_000086.7 |
X |
107677461 |
- |
Tbx22 |
NNNGTGATTTTTAATGAAGG |
NGG |
2 |
0.3394 |
Tier II |
| 7 |
NC_000072.6 |
6 |
46480076 |
+ |
Cntnap2 |
NNNGTGATGAGGAATGAAGG |
NGG |
2 |
0.3095 |
Tier II |
| 8 |
NC_000082.6 |
16 |
45386968 |
+ |
Cd200 |
NNNGTGATTAGTAATGAAGA |
NAG |
2 |
0.2431 |
Tier II |
| 9 |
NC_000072.6 |
6 |
27480615 |
- |
Grm8 |
NNNGTGATTACTAATGAAGC |
NGG |
2 |
0.1837 |
Tier II |
| 10 |
NC_000070.6 |
4 |
66052346 |
- |
Astn2 |
NNNGTGATTAGGTATGAAGG |
NGG |
2 |
0.15 |
Tier II |
| 11 |
NC_000070.6 |
4 |
85751903 |
- |
Adamtsl1 |
NNNGTGATTTGTAATGTAGG |
NGG |
2 |
0.1176 |
Tier II |
| 12 |
NC_000084.6 |
18 |
45477216 |
- |
Kcnn2 |
NNNGTGATAAGTAATGAAGG |
NTG |
2 |
0.0362 |
Tier II |
| 13 |
NC_000075.6 |
9 |
10449276 |
+ |
Cntn5 |
NNNGTGCTTAGTAAGGAAGG |
NGG |
2 |
0.0219 |
Tier II |
| 14 |
NC_000082.6 |
16 |
66975977 |
+ |
Cadm2 |
NNNGTGATTAGAAATGAAGG |
NGT |
2 |
0.0129 |
Tier II |
| 15 |
NC_000072.6 |
6 |
51280171 |
- |
Gm38821 |
NNNGTGATTAGTACTGAAGG |
NGT |
2 |
0.0035 |
Tier II |
| 16 |
NC_000078.6 |
12 |
26212671 |
- |
4930480M12Rik |
NNNGAGATCAGTAATGAAGG |
NGG |
2 |
0.4615 |
Tier III |
| 17 |
NC_000070.6 |
4 |
11033356 |
- |
Gm35429 |
NNNGTTATTGGTAATGAAGG |
NGG |
2 |
0.3704 |
Tier III |
| 18 |
NC_000084.6 |
18 |
45477216 |
- |
Gm31907 |
NNNGTGATAAGTAATGAAGG |
NTG |
2 |
0.0362 |
Tier III |
| 19 |
NC_000073.6 |
7 |
78706267 |
- |
E430016F16Rik |
NNNGTGATTAGTAATGATGG |
NGT |
2 |
0.0081 |
Tier III |
Other clones with same target sequence:
(none)
