Construct: sgRNA BRDN0001147251
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTGGGCAAAGCTTCAAGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- VRK3 (51231)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75551
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
50007671 |
+ |
VRK3 |
NNNGGGCAAAGCTTCAAGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
27834784 |
+ |
RBKS |
NNNGAGCAAAGCTACAAGTG |
NGG |
2 |
0.5365 |
Tier II |
| 3 |
NC_000008.11 |
8 |
91185748 |
- |
LRRC69 |
NNNGGGAAAAGATTCAAGTG |
NGG |
2 |
0.5357 |
Tier II |
| 4 |
NC_000023.11 |
X |
51894074 |
- |
MAGED1 |
NNNGGGCGAAGCTTCAAATG |
NGG |
2 |
0.5077 |
Tier II |
| 5 |
NC_000013.11 |
13 |
109165740 |
- |
MYO16 |
NNNGAGCCAAGCTTCAAGTG |
NGG |
2 |
0.3714 |
Tier II |
| 6 |
NC_000011.10 |
11 |
18491009 |
+ |
TSG101 |
NNNGAGCAAAGCTCCAAGTG |
NGG |
2 |
0.2476 |
Tier II |
| 7 |
NC_000007.14 |
7 |
30147504 |
+ |
MTURN |
NNNGGGCCCAGCTTCAAGTG |
NGG |
2 |
0.2449 |
Tier II |
| 8 |
NC_000022.11 |
22 |
33536519 |
- |
LARGE1 |
NNNTGGCAAAGCTACAAGTG |
NGG |
2 |
0.2251 |
Tier II |
| 9 |
NC_000002.12 |
2 |
201806634 |
+ |
CDK15 |
NNNGGGGAAAGGTTCAAGTG |
NGG |
2 |
0.2092 |
Tier II |
| 10 |
NC_000003.12 |
3 |
12998931 |
- |
IQSEC1 |
NNNGGGCAAAGCTTCATGTT |
NGG |
2 |
0.0933 |
Tier II |
| 11 |
NC_000014.9 |
14 |
71908289 |
+ |
RGS6 |
NNNCGGCAAAGCTTCAAGTG |
NGA |
2 |
0.0368 |
Tier II |
| 12 |
NC_000009.12 |
9 |
36962082 |
+ |
PAX5 |
NNNGGGAAAAGCTGCAAGTG |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000015.10 |
15 |
96324125 |
- |
NR2F2-AS1 |
NNNGGGCACAGCATCAAGTG |
NGG |
2 |
0.3956 |
Tier III |
| 14 |
NC_000013.11 |
13 |
109165740 |
- |
MYO16-AS1 |
NNNGAGCCAAGCTTCAAGTG |
NGG |
2 |
0.3714 |
Tier III |
| 15 |
NC_000011.10 |
11 |
18491009 |
+ |
YWHABP2 |
NNNGAGCAAAGCTCCAAGTG |
NGG |
2 |
0.2476 |
Tier III |
| 16 |
NC_000007.14 |
7 |
134731130 |
- |
LOC124901750 |
NNNGGGCAAAGCTTCAAGGC |
NGG |
2 |
0.1071 |
Tier III |
| 17 |
NC_000010.11 |
10 |
2520256 |
+ |
LOC105376350 |
NNNGGACAAAGCTGCAAGTG |
NGG |
2 |
0.0 |
Tier III |
| 18 |
NC_000002.12 |
2 |
3100149 |
+ |
LINC01250 |
NNNGGGCGAAGCTGCAAGTG |
NGG |
2 |
0.0 |
Tier III |
| 19 |
NC_000002.12 |
2 |
5675396 |
+ |
LINC01248 |
NNNGGTCAAAGCTGCAAGTG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
121026827 |
+ |
Epb42 |
NNNAGGCAAAACTTCAAGTG |
NGG |
2 |
0.9 |
Tier II |
| 2 |
NC_000070.6 |
4 |
106452518 |
- |
Pcsk9 |
NNNGAGCAAAACTTCAAGTG |
NGG |
2 |
0.8667 |
Tier II |
| 3 |
NC_000074.6 |
8 |
65895976 |
+ |
Marchf1 |
NNNGAGCAAAGCTACAAGTG |
NGG |
2 |
0.5365 |
Tier II |
| 4 |
NC_000079.6 |
13 |
36058586 |
- |
Lyrm4 |
NNNGGGCAGAGCTTCAAATG |
NGG |
2 |
0.4615 |
Tier II |
| 5 |
NC_000072.6 |
6 |
54078757 |
- |
Chn2 |
NNNGGGTAAATCTTCAAGTG |
NGG |
2 |
0.3125 |
Tier II |
| 6 |
NC_000070.6 |
4 |
57438640 |
- |
Pakap |
NNNAGGCAAAGCTTCAAGAG |
NGG |
2 |
0.2571 |
Tier II |
| 7 |
NC_000077.6 |
11 |
94452177 |
- |
Cacna1g |
NNNGGGCAAAGCTTCATGGG |
NGG |
2 |
0.0333 |
Tier II |
| 8 |
NC_000084.6 |
18 |
39080997 |
- |
Arhgap26 |
NNNGGGCAAACCTTCCAGTG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000068.7 |
2 |
62359462 |
- |
Dpp4 |
NNNGGGCAAAGCAGCAAGTG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000072.6 |
6 |
77013412 |
+ |
Ctnna2 |
NNNGGGCAAAGCTGCAAATG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000068.7 |
2 |
91237439 |
+ |
A330069E16Rik |
NNNGAGCAAAGATTCAAGTG |
NGG |
2 |
0.619 |
Tier III |
| 12 |
NC_000070.6 |
4 |
129275693 |
+ |
Gm12976 |
NNNAGGCAGAGCTTCAAGTG |
NGG |
2 |
0.6 |
Tier III |
| 13 |
NC_000068.7 |
2 |
121937899 |
+ |
Gm39916 |
NNNAGGCAAGGCTTCAAGTG |
NGG |
2 |
0.5 |
Tier III |
| 14 |
NC_000079.6 |
13 |
5956108 |
+ |
Gm46401 |
NNNGGGGAAAGCTTCAAGTG |
NAG |
2 |
0.122 |
Tier III |
| 15 |
NC_000082.6 |
16 |
87843769 |
- |
2810407A14Rik |
NNNGGGCAAAGCTTCATCTG |
NGG |
2 |
0.0635 |
Tier III |
| 16 |
NC_000081.6 |
15 |
75278932 |
+ |
Gm52218 |
NNNGGGCAAAGCATCCAGTG |
NGG |
2 |
0.0 |
Tier III |
| 17 |
NC_000085.6 |
19 |
58609627 |
- |
1810007D17Rik |
NNNGGGCAAAGCTTCCGGTG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
