Construct: sgRNA BRDN0001147255
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTCTGGAGTACTGTAGTGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CHEK1 (1111)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76642
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
125627805 |
+ |
CHEK1 |
NNNCTGGAGTACTGTAGTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
10827736 |
+ |
MAK |
NNNCTGAAGTACAGTAGTGG |
NGG |
2 |
0.6923 |
Tier II |
| 3 |
NC_000007.14 |
7 |
30141896 |
- |
MTURN |
NNNCTGGAGTACTGAAGTGG |
NGG |
1 |
0.5789 |
Tier II |
| 4 |
NC_000002.12 |
2 |
154771904 |
+ |
KCNJ3 |
NNNCTGGAGTACAATAGTGG |
NGG |
2 |
0.5192 |
Tier II |
| 5 |
NC_000007.14 |
7 |
37114326 |
+ |
ELMO1 |
NNNCTGGATTACTGTAATGG |
NGG |
2 |
0.4978 |
Tier II |
| 6 |
NC_000002.12 |
2 |
218519695 |
+ |
USP37 |
NNNCTGGAGTGCAGTAGTGG |
NGG |
2 |
0.45 |
Tier II |
| 7 |
NC_000020.11 |
20 |
54042126 |
+ |
BCAS1 |
NNNCTGGACTACTGTAGTTG |
NGG |
2 |
0.359 |
Tier II |
| 8 |
NC_000007.14 |
7 |
30138604 |
+ |
MTURN |
NNNCTGTAGTACTGAAGTGG |
NGG |
2 |
0.3308 |
Tier II |
| 9 |
NC_000001.11 |
1 |
72094996 |
- |
NEGR1 |
NNNCTTGTGTACTGTAGTGG |
NGG |
2 |
0.2857 |
Tier II |
| 10 |
NC_000003.12 |
3 |
24323707 |
- |
THRB |
NNNCTGGAGTATTCTAGTGG |
NGG |
2 |
0.2308 |
Tier II |
| 11 |
NC_000006.12 |
6 |
3748602 |
- |
PXDC1 |
NNNCTTGAGTACTGCAGTGG |
NGG |
2 |
0.1818 |
Tier II |
| 12 |
NC_000010.11 |
10 |
126644303 |
- |
C10orf90 |
NNNCTGGAGTTCTGCAGTGG |
NGG |
2 |
0.0839 |
Tier II |
| 13 |
NC_000001.11 |
1 |
46256592 |
- |
RAD54L |
NNNCTGGAGTACAGTAGTGG |
NGA |
2 |
0.0481 |
Tier II |
| 14 |
NC_000010.11 |
10 |
48790068 |
+ |
WDFY4 |
NNNCTGGAGTTCTGGAGTGG |
NGG |
2 |
0.0154 |
Tier II |
| 15 |
NC_000007.14 |
7 |
2999634 |
+ |
CARD11 |
NNNCTGGAGTGCTGTAGTGG |
NGC |
2 |
0.0144 |
Tier II |
| 16 |
NC_000001.11 |
1 |
12885417 |
+ |
PRAMEF4 |
NNNCTGGAGTACAGTAGTGG |
NGT |
2 |
0.0112 |
Tier II |
| 17 |
NC_000006.12 |
6 |
35224589 |
- |
SCUBE3 |
NNNCTGGAGTACAGTAGTGG |
NGT |
2 |
0.0112 |
Tier II |
| 18 |
NC_000022.11 |
22 |
25579217 |
+ |
GRK3 |
NNNCTGGAGTACAGTAGTGG |
NGT |
2 |
0.0112 |
Tier II |
| 19 |
NC_000001.11 |
1 |
16416639 |
- |
SPATA21 |
NNNCTGGAGTGCTGTAGTGG |
NGT |
2 |
0.0105 |
Tier II |
| 20 |
NC_000001.11 |
1 |
7909505 |
- |
UTS2 |
NNNCTGGAGTAGTGTAGTGG |
NGC |
2 |
0.0099 |
Tier II |
| 21 |
NC_000004.12 |
4 |
83577857 |
+ |
GPAT3 |
NNNCTGGAGTAGTGTAGTGG |
NGC |
2 |
0.0099 |
Tier II |
| 22 |
NC_000001.11 |
1 |
156483995 |
+ |
MEF2D |
NNNCTGGAGTCCTGTAGTGG |
NGC |
2 |
0.0089 |
Tier II |
| 23 |
NC_000013.11 |
13 |
51118348 |
- |
C13orf42 |
NNNCTGGAGGACTGTTGTGG |
NGG |
2 |
0.0 |
Tier II |
| 24 |
NC_000008.11 |
8 |
16555584 |
+ |
LOC101929028 |
NNNCTGGATTACTGAAGTGG |
NGG |
2 |
0.3088 |
Tier III |
| 25 |
NC_000019.10 |
19 |
31717732 |
- |
LOC105372361 |
NNNCTGGAGTACTGGAGTGA |
NGG |
2 |
0.0469 |
Tier III |
| 26 |
NC_000012.12 |
12 |
63812234 |
+ |
RXYLT1-AS1 |
NNNCTGGAGTACAGTAGTGG |
NGC |
2 |
0.0154 |
Tier III |
| 27 |
NC_000006.12 |
6 |
35224589 |
- |
SCUBE3-AS1 |
NNNCTGGAGTACAGTAGTGG |
NGT |
2 |
0.0112 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
36723823 |
- |
Chek1 |
NNNCTGGAGTACTGTAGTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000076.6 |
10 |
3267246 |
- |
H60c |
NNNCTGGAGTCCTGTAGTGC |
NGG |
2 |
0.1714 |
Tier I |
| 3 |
NC_000076.6 |
10 |
75517841 |
- |
Gucd1 |
NNNCCTGAGTACTGTAGTGG |
NGG |
2 |
0.6667 |
Tier II |
| 4 |
NC_000081.6 |
15 |
4089849 |
+ |
Oxct1 |
NNNCTGGAGAACAGTAGTGG |
NGG |
2 |
0.5934 |
Tier II |
| 5 |
NC_000074.6 |
8 |
118144517 |
- |
Cdh13 |
NNNCTGAAGTACTGAAGTGG |
NGG |
2 |
0.5789 |
Tier II |
| 6 |
NC_000075.6 |
9 |
58025427 |
+ |
Cyp11a1 |
NNNCTGGGGTGCTGTAGTGG |
NGG |
2 |
0.4767 |
Tier II |
| 7 |
NC_000070.6 |
4 |
43006172 |
- |
Fancg |
NNNCTGGAGTACTGTAGTTT |
NGG |
2 |
0.4667 |
Tier II |
| 8 |
NC_000076.6 |
10 |
77592017 |
+ |
Pttg1ip |
NNNCTTGAGTACAGTAGTGG |
NGG |
2 |
0.4615 |
Tier II |
| 9 |
NC_000077.6 |
11 |
35374281 |
+ |
Slit3 |
NNNTTGTAGTACTGTAGTGG |
NGG |
2 |
0.4571 |
Tier II |
| 10 |
NC_000084.6 |
18 |
25610194 |
+ |
Celf4 |
NNNCTGGAGTTCTGTAGTGG |
NGG |
1 |
0.3077 |
Tier II |
| 11 |
NC_000071.6 |
5 |
140191435 |
- |
Mad1l1 |
NNNCTGGCTTACTGTAGTGG |
NGG |
2 |
0.2286 |
Tier II |
| 12 |
NC_000072.6 |
6 |
113843256 |
- |
Atp2b2 |
NNNCTGGAGGACTGTGGTGG |
NGG |
2 |
0.0962 |
Tier II |
| 13 |
NC_000080.6 |
14 |
77911475 |
- |
Epsti1 |
NNNCTGGAGCACTGTAGTGG |
NGA |
2 |
0.037 |
Tier II |
| 14 |
NC_000082.6 |
16 |
45918795 |
+ |
Phldb2 |
NNNCTGGAATACTGGAGTGG |
NGG |
2 |
0.0321 |
Tier II |
| 15 |
NC_000069.6 |
3 |
68603651 |
- |
Schip1 |
NNNCTGGAGTACTGTAGTGC |
NGA |
2 |
0.0298 |
Tier II |
| 16 |
NC_000069.6 |
3 |
68603651 |
- |
Iqschfp |
NNNCTGGAGTACTGTAGTGC |
NGA |
2 |
0.0298 |
Tier II |
| 17 |
NC_000073.6 |
7 |
92201302 |
+ |
Dlg2 |
NNNCTGGAGTACTGGAGTGC |
NGG |
2 |
0.0214 |
Tier II |
| 18 |
NC_000079.6 |
13 |
35855217 |
+ |
Cdyl |
NNNCTGGAGTACAGTAGTGG |
NGT |
2 |
0.0112 |
Tier II |
| 19 |
NC_000074.6 |
8 |
63210052 |
+ |
Spock3 |
NNNCTGGTGTACTGTAGTGG |
NGT |
2 |
0.0069 |
Tier II |
| 20 |
NC_000082.6 |
16 |
11878852 |
+ |
Cpped1 |
NNNCTGGAGTACTGGAGTGG |
NTG |
2 |
0.0019 |
Tier II |
| 21 |
NC_000069.6 |
3 |
87760609 |
- |
Pear1 |
NNNCTGGAGTACTGTTCTGG |
NGG |
2 |
0.0 |
Tier II |
| 22 |
NC_000069.6 |
3 |
34009996 |
+ |
Gm46798 |
NNNCTGGAGTACTGTAGAGG |
NTG |
2 |
0.026 |
Tier III |
Other clones with same target sequence:
(none)
