Construct: sgRNA BRDN0001147256
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAAGCGAGTTCAATCTCTTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PFKFB2 (5208)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77688
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
207067659 |
+ |
PFKFB2 |
NNNGCGAGTTCAATCTCTTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
6608839 |
+ |
MAN2B2 |
NNNGCCAGTTCCATCTCTTG |
NGG |
2 |
0.1794 |
Tier II |
| 3 |
NC_000003.12 |
3 |
76005042 |
+ |
ROBO2 |
NNNGGGAGTTCAATCTCTTG |
NTG |
2 |
0.0234 |
Tier II |
| 4 |
NC_000023.11 |
X |
8428489 |
- |
LOC107985675 |
NNNTTGAGTTCAATCTCTTG |
NGG |
2 |
0.2314 |
Tier III |
| 5 |
NC_000020.11 |
20 |
49769454 |
- |
LOC105372652 |
NNNGCGAGTTCAATCTCTTG |
NTC |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
130702279 |
- |
Pfkfb2 |
NNNGCGAGTTCAACCTTTTG |
NGG |
2 |
0.1333 |
Tier I |
| 2 |
NC_000067.6 |
1 |
93421833 |
- |
Hdlbp |
NNNGCGAGTTAAATCTCTTT |
NGG |
2 |
0.525 |
Tier II |
| 3 |
NC_000072.6 |
6 |
38011087 |
- |
Svopl |
NNNGTGAGTTCTATCTCTTG |
NGG |
2 |
0.2121 |
Tier II |
| 4 |
NC_000075.6 |
9 |
121083871 |
+ |
Ulk4 |
NNNGAGAGTTCACTCTCTTG |
NGG |
2 |
0.1203 |
Tier II |
| 5 |
NC_000067.6 |
1 |
93421833 |
- |
Gm17415 |
NNNGCGAGTTAAATCTCTTT |
NGG |
2 |
0.525 |
Tier III |
Other clones with same target sequence:
(none)
