Construct: sgRNA BRDN0001147258
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGACAGAGCCATATACTGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ITPKC (80271)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77650
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
40717873 |
+ |
ITPKC |
NNNACAGAGCCATATACTGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
147619760 |
+ |
BCL9 |
NNNACAGAGCCATTTTCTGA |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000005.10 |
5 |
50433973 |
- |
EMB |
NNNACAGAGACATATACAGA |
NGG |
2 |
0.5778 |
Tier II |
| 4 |
NC_000011.10 |
11 |
101489535 |
- |
TRPC6 |
NNNACAGAGCCATATACAAA |
NGG |
2 |
0.4762 |
Tier II |
| 5 |
NC_000003.12 |
3 |
54204350 |
- |
CACNA2D3 |
NNNACAGAGCCATATACATA |
NGG |
2 |
0.4444 |
Tier II |
| 6 |
NC_000010.11 |
10 |
22213193 |
- |
EBLN1 |
NNNAAAGATCCATATACTGA |
NGG |
2 |
0.3048 |
Tier II |
| 7 |
NC_000013.11 |
13 |
113016957 |
+ |
MCF2L |
NNNTCAGAGCCATGTACTGA |
NGG |
2 |
0.297 |
Tier II |
| 8 |
NC_000011.10 |
11 |
133212699 |
- |
OPCML |
NNNACAGAGCCATATAATCA |
NGG |
2 |
0.2092 |
Tier II |
| 9 |
NC_000010.11 |
10 |
31009373 |
+ |
ZNF438 |
NNNAAAGAGCCATATACTGA |
NAG |
2 |
0.1481 |
Tier II |
| 10 |
NC_000007.14 |
7 |
89155630 |
- |
ZNF804B |
NNNACAGAGCCAGATAATGA |
NGG |
2 |
0.1217 |
Tier II |
| 11 |
NC_000012.12 |
12 |
70781377 |
+ |
PTPRR |
NNNACAGAGCCAGATACTGC |
NGG |
2 |
0.0593 |
Tier II |
| 12 |
NC_000007.14 |
7 |
14870191 |
+ |
DGKB |
NNNACAGAGCCATATACTCA |
NTG |
2 |
0.0175 |
Tier II |
| 13 |
NC_000006.12 |
6 |
152389492 |
- |
SYNE1 |
NNNAAAGAGCCATATTCTGA |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000018.10 |
18 |
48797610 |
+ |
CTIF |
NNNACAGAGCCTTATCCTGA |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000001.11 |
1 |
121526518 |
+ |
EMBP1 |
NNNACAGAGACATATACAGA |
NGG |
2 |
0.5778 |
Tier III |
| 16 |
NC_000017.11 |
17 |
72099641 |
- |
SOX9-AS1 |
NNNACACAGCCATATACAGA |
NGG |
2 |
0.4583 |
Tier III |
| 17 |
NC_000006.12 |
6 |
15170348 |
+ |
LOC105374946 |
NNNACAGAGCCTGATACTGA |
NGG |
2 |
0.087 |
Tier III |
| 18 |
NC_000008.11 |
8 |
137953823 |
+ |
LOC401478 |
NNNACAGAGCCATATCATGA |
NGG |
2 |
0.0 |
Tier III |
| 19 |
NC_000019.10 |
19 |
54567288 |
- |
LOC105372461 |
NNNACAGAGCCATATTCTCA |
NGG |
2 |
0.0 |
Tier III |
| 20 |
NC_000018.10 |
18 |
48797610 |
+ |
LOC105372106 |
NNNACAGAGCCTTATCCTGA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
43991793 |
- |
Rcan2 |
NNNACAGAGTCATATACTAA |
NGG |
2 |
0.6723 |
Tier II |
| 2 |
NC_000073.6 |
7 |
43358366 |
+ |
Siglecf |
NNNACAGAGCCACATACTGG |
NGG |
2 |
0.6037 |
Tier II |
| 3 |
NC_000077.6 |
11 |
102401779 |
+ |
Rundc3a |
NNNACAGAGACATAAACTGA |
NGG |
2 |
0.5018 |
Tier II |
| 4 |
NC_000076.6 |
10 |
119949817 |
- |
Grip1 |
NNNACAGAGCCATATACTCA |
NGG |
1 |
0.4483 |
Tier II |
| 5 |
NC_000070.6 |
4 |
83594699 |
- |
Ccdc171 |
NNNCCAGAGTCATATACTGA |
NGG |
2 |
0.3322 |
Tier II |
| 6 |
NC_000081.6 |
15 |
85409668 |
- |
Atxn10 |
NNNACAGAGCCATATATTTA |
NGG |
2 |
0.3111 |
Tier II |
| 7 |
NC_000067.6 |
1 |
164397167 |
- |
Nme7 |
NNNACAGAGACAGATACTGA |
NGG |
2 |
0.2261 |
Tier II |
| 8 |
NC_000068.7 |
2 |
157821510 |
- |
Ctnnbl1 |
NNNACAGAGCCATATGCTGA |
NGG |
1 |
0.1923 |
Tier II |
| 9 |
NC_000075.6 |
9 |
29025929 |
- |
Ntm |
NNNACAGAGGCATATACTGA |
NTG |
2 |
0.0152 |
Tier II |
| 10 |
NC_000072.6 |
6 |
113261917 |
- |
Mtmr14 |
NNNACAGAGCCAAATTCTGA |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000075.6 |
9 |
62429382 |
- |
Coro2b |
NNNACAGAGCCACATTCTGA |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000080.6 |
14 |
55645681 |
+ |
Tssk4 |
NNNACAGAGCCATATCCTGA |
NGC |
2 |
0.0 |
Tier II |
| 13 |
NC_000078.6 |
12 |
5619410 |
- |
Gm32398 |
NNNACTGAGCCATATACTGA |
NGG |
1 |
0.7143 |
Tier III |
| 14 |
NC_000082.6 |
16 |
31499879 |
- |
Gm34032 |
NNNACAGAACCATATACTAA |
NGG |
2 |
0.4592 |
Tier III |
| 15 |
NC_000069.6 |
3 |
49345779 |
- |
Gm20557 |
NNNAGAGAGCCATATACTGA |
NAG |
2 |
0.1556 |
Tier III |
Other clones with same target sequence:
(none)
