Construct: sgRNA BRDN0001147259
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAAGCACTGCAGAATCGAGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DYRK2 (8445)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77536
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
67657899 |
- |
DYRK2 |
NNNGCACTGCAGAATCGAGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
26340377 |
+ |
SNX10 |
NNNGCACTGCAGACTTGAGT |
NGG |
2 |
0.0659 |
Tier II |
| 3 |
NC_000006.12 |
6 |
144763046 |
- |
UTRN |
NNNGCACTGCAGACTCCAGT |
NGG |
2 |
0.0504 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
118860367 |
+ |
Dyrk2 |
NNNGCACTGCAGAATGGAGT |
NGG |
1 |
0.1538 |
Tier I |
| 2 |
NC_000075.6 |
9 |
59033997 |
- |
Neo1 |
NNNGCACTGCAAAATAGAGT |
NGG |
2 |
0.9333 |
Tier II |
| 3 |
NC_000071.6 |
5 |
147363443 |
+ |
Flt3 |
NNNGAACTGCAGAATCTAGT |
NGG |
2 |
0.1429 |
Tier II |
| 4 |
NC_000068.7 |
2 |
121994259 |
+ |
Ctdspl2 |
NNNGAACTGCAGAATCCAGT |
NGG |
2 |
0.1345 |
Tier II |
| 5 |
NC_000073.6 |
7 |
91474900 |
- |
Dlg2 |
NNNGCACTGCAGAATCGATT |
NTG |
2 |
0.026 |
Tier II |
| 6 |
NC_000071.6 |
5 |
142888386 |
- |
Fbxl18 |
NNNGCACTGCAGAATGGAGT |
NGC |
2 |
0.0034 |
Tier II |
Other clones with same target sequence:
(none)
