Construct: sgRNA BRDN0001147260

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAATAACAGACCTCAAAGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PFKFB2 (5208)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77687

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 207068217 + PFKFB2 NNNTAACAGACCTCAAAGTG NGG 0 1.0 Tier I
2 NC_000011.10 11 34179388 - ABTB2 NNNCAACAGTCCTCAAAGTG NGG 2 0.5709 Tier II
3 NC_000011.10 11 86398930 - CCDC81 NNNGAACAGTCCTCAAAGTG NGG 2 0.5515 Tier II
4 NC_000006.12 6 20121391 + MBOAT1 NNNTAACAAACCTCAAATTG NGG 2 0.4286 Tier II
5 NC_000023.11 X 21619964 - CNKSR2 NNNTAACAGAATTCAAAGTG NGG 2 0.4038 Tier II
6 NC_000019.10 19 19432712 + GATAD2A NNNAAAAAGACCTCAAAGTG NGG 2 0.3571 Tier II
7 NC_000012.12 12 25582530 + LMNTD1 NNNTAGGAGACCTCAAAGTG NGG 2 0.3361 Tier II
8 NC_000004.12 4 157091645 - GLRB NNNTAACAGCCCTCAAAGTG NGG 1 0.3333 Tier II
9 NC_000005.10 5 119511427 + HSD17B4 NNNTAACAAACCTCAAAGTC NGG 2 0.2755 Tier II
10 NC_000005.10 5 108943436 - FER NNNTAACAGTTCTCAAAGTG NGG 2 0.2715 Tier II
11 NC_000005.10 5 58632305 + RAB3C NNNCTACAGACCTCAAAGTG NGG 2 0.2353 Tier II
12 NC_000010.11 10 126259167 + ADAM12 NNNTAACAGACGTCAAACTG NGG 2 0.2116 Tier II
13 NC_000007.14 7 18407435 + HDAC9 NNNTAACAAATCTCAAAGTG NGG 2 0.1978 Tier II
14 NC_000001.11 1 53198971 - CPT2 NNNTACCAGACCTCAAAGTC NGG 2 0.1948 Tier II
15 NC_000011.10 11 8383997 - STK33 NNNTATCAGACCTCAAAGTG NAG 2 0.1852 Tier II
16 NC_000002.12 2 46480079 + TMEM247 NNNTAACTGACCTCAAAGTC NGG 2 0.1837 Tier II
17 NC_000001.11 1 153941556 - DENND4B NNNTAACAGGTCTCAAAGTG NGG 2 0.1709 Tier II
18 NC_000018.10 18 63422352 + VPS4B NNNTAACAGCCCTCAAACTG NGG 2 0.1587 Tier II
19 NC_000019.10 19 19584597 + PBX4 NNNCAACAGACCTCAAGGTG NGG 2 0.1142 Tier II
20 NC_000012.12 12 55692532 + ITGA7 NNNAAACAGACCTCAAAGTG NGA 2 0.0331 Tier II
21 NC_000017.11 17 31257969 - NF1 NNNTAACAGACATGAAAGTG NGG 2 0.0 Tier II
22 NC_000009.12 9 101508552 - PGAP4 NNNTAACAGACCTCACAGGG NGG 2 0.0 Tier II
23 NC_000008.11 8 20109356 + LOC105379311 NNNTAACATACCTCAAAATG NGG 2 0.3692 Tier III
24 NC_000016.10 16 9403516 + LINC02177 NNNGAAGAGACCTCAAAGTG NGG 2 0.2941 Tier III
25 NC_000008.11 8 64341693 - LINC01414 NNNTTACACACCTCAAAGTG NGG 2 0.1958 Tier III
26 NC_000002.12 2 74957636 + LOC105374809 NNNTATCAGACCTCCAAGTG NGG 2 0.1948 Tier III
27 NC_000003.12 3 81090138 + LINC02027 NNNTAACTGATCTCAAAGTG NGG 2 0.1319 Tier III
28 NC_000014.9 14 29322919 + LOC102724934 NNNTAACAGACCTCCAAGTG NTG 2 0.0106 Tier III
29 NC_000008.11 8 93297403 - LOC107986956 NNNAAACAGACCTGAAAGTG NGG 2 0.0 Tier III
30 NC_000015.10 15 20924303 + NF1P1 NNNTAACAGACATGAAAGTG NGG 2 0.0 Tier III
31 NC_000015.10 15 21498003 + LOC105369227 NNNTAACAGACATGAAAGTG NGG 2 0.0 Tier III
32 NC_000015.10 15 21935283 + NF1P2 NNNTAACAGACATGAAAGTG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 81628844 - Inpp4b NNNTAACAGACATCAAAGTT NGG 2 0.5 Tier II
2 NC_000080.6 14 63984181 + 4930578I06Rik NNNTAAAAGACTTCAAAGTG NGG 2 0.4038 Tier II
3 NC_000077.6 11 19965659 - Spred2 NNNAAACAGACCACAAAGTG NGG 2 0.3297 Tier II
4 NC_000076.6 10 119508413 + Grip1 NNNAAACAGACCTCAAATTG NGG 2 0.3175 Tier II
5 NC_000083.6 17 83770559 - Mta3 NNNTAACTGACCTCAAAATG NGG 2 0.2967 Tier II
6 NC_000068.7 2 27711041 - Rxra NNNTGACAGACCTAAAAGTG NGG 2 0.252 Tier II
7 NC_000073.6 7 141870470 - Muc5b NNNAAACAGACCTCAAACTG NGG 2 0.2268 Tier II
8 NC_000081.6 15 102088839 - Eif4b NNNTAACAGATCTCAAATTG NGG 2 0.2051 Tier II
9 NC_000067.6 1 84532657 + Dner NNNTAACAGAGCTCAAAATG NGG 2 0.1731 Tier II
10 NC_000070.6 4 19311235 + Cngb3 NNNTAACAGACCGCAAAGAG NGG 2 0.0745 Tier II
11 NC_000069.6 3 25866212 - Nlgn1 NNNTAACAGACCTCAAAGTT NGT 2 0.0113 Tier II
12 NC_000071.6 5 114768003 + Git2 NNNTAACAGAACTCACAGTG NGG 2 0.0 Tier II
13 NC_000077.6 11 30902394 - Erlec1 NNNTAACAGAACTGAAAGTG NGG 2 0.0 Tier II
14 NC_000077.6 11 33306864 + Ranbp17 NNNTAACAGACATCATAGTG NGG 2 0.0 Tier II
15 NC_000080.6 14 63984181 + Gm36410 NNNTAAAAGACTTCAAAGTG NGG 2 0.4038 Tier III
16 NC_000078.6 12 57979244 + Gm46329 NNNAATCAGACCTCAAAGTG NGG 2 0.3401 Tier III
17 NC_000082.6 16 5405304 - Gm41414 NNNTAACAGATCTCAAAGTG NGG 1 0.3077 Tier III
18 NC_000082.6 16 55527230 + Gm45968 NNNTAAAAGACCTCAAAGTG NGA 2 0.0521 Tier III
19 NC_000078.6 12 110324264 - Gm9063 NNNTAAGAGACCTCAAAGTG NGC 2 0.0105 Tier III
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Other clones with same target sequence:

(none)