GPP Web Portal - Clone Details

Construct: sgRNA BRDN0001147265

Construct Description:

Construct Type:: sgRNA
Other Identifiers:: n/a
DNA Barcode:: CCAAGGCTATGACACAAAGT

Original Target:

Taxon:: Homo sapiens (human)
Gene:: NEK11 (79858)
Gene Description:: n/a
CRISPR Mechanism: CRISPRko

Vector Information:

Vector Backbone:: pXPR_003
Pol II Cassette 1:: EF1A-PuroR
Pol II Cassette 2:: n/a
Selection Marker:: PuroR
Visible Reporter:: n/a
Epitope Tag:: n/a

Additional Information:

Addgene ID:: 75563

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000003.12	3	131133944	+	NEK11	NNNAGGCTATGACACAAAGT	NGG	0	1.0	Tier I
2	NC_000007.14	7	104690415	-	LHFPL3	NNNAGATTATGACACAAAGT	NGG	2	0.8125	Tier II
3	NC_000013.11	13	42939762	+	EPSTI1	NNNACACTATGACACAAAGT	NGG	2	0.7857	Tier II
4	NC_000002.12	2	214781699	-	BARD1	NNNAGGATATGGCACAAAGT	NGG	2	0.5417	Tier II
5	NC_000001.11	1	52362437	+	CC2D1B	NNNAGGCCATGACACAAGGT	NGG	2	0.4	Tier II
6	NC_000003.12	3	58308261	-	RPP14	NNNATACTATGACACAAAGT	NGG	2	0.3	Tier II
7	NC_000003.12	3	58308261	-	HTD2	NNNATACTATGACACAAAGT	NGG	2	0.3	Tier II
8	NC_000017.11	17	45483991	+	PLEKHM1	NNNAGGCCATGACACGAAGT	NGG	2	0.1923	Tier II
9	NC_000005.10	5	68266933	-	PIK3R1	NNNAGGCCATGACACACAGT	NGG	2	0.1765	Tier II
10	NC_000015.10	15	51250632	+	CYP19A1	NNNAGGCCATGAGACAAAGT	NGG	2	0.1364	Tier II
11	NC_000019.10	19	12693357	-	FBXW9	NNNAGGCTTTGACACACAGT	NGG	2	0.1059	Tier II
12	NC_000003.12	3	3831865	+	LRRN1	NNNAGACTATGACACAAAGC	NGG	2	0.0909	Tier II
13	NC_000003.12	3	50241759	-	GNAI2	NNNAGGCTCTGACACATAGT	NGG	2	0.0762	Tier II
14	NC_000009.12	9	9778792	+	PTPRD	NNNAGGCTATGGCACAAAGT	NTG	2	0.0281	Tier II
15	NC_000001.11	1	240873450	+	RGS7	NNNAGGCTATGCCACTAAGT	NGG	2	0.0	Tier II
16	NC_000013.11	13	30899836	-	TEX26-AS1	NNNAGACTAAGACACAAAGT	NGG	2	0.8571	Tier III
17	NC_000003.12	3	55263458	-	LINC02030	NNNAGATTATGACACAAAGT	NGG	2	0.8125	Tier III
18	NC_000003.12	3	55263458	-	LOC124906243	NNNAGATTATGACACAAAGT	NGG	2	0.8125	Tier III
19	NC_000012.12	12	74036719	-	LINC02394	NNNAGATTATGACACAAAGT	NGG	2	0.8125	Tier III
20	NC_000014.9	14	81845904	+	LOC107984704	NNNAGAGTATGACACAAAGT	NGG	2	0.4706	Tier III
21	NC_000009.12	9	22708312	+	LINC01239	NNNAGGCTATGACTCAATGT	NGG	2	0.2667	Tier III
22	NC_000009.12	9	118680898	+	LOC102724929	NNNAGGCTATGACACAAATT	NAG	2	0.1728	Tier III
23	NC_000009.12	9	118680898	+	LINC02578	NNNAGGCTATGACACAAATT	NAG	2	0.1728	Tier III
24	NC_000007.14	7	138017297	-	LOC124901752	NNNAGGTTATGACACAAAGG	NGG	2	0.1434	Tier III
25	NC_000015.10	15	51250632	+	MIR4713HG	NNNAGGCCATGAGACAAAGT	NGG	2	0.1364	Tier III
26	NC_000002.12	2	67801415	-	LINC01812	NNNAGTCTATGACACAAAGG	NGG	2	0.1176	Tier III
27	NC_000008.11	8	136514192	-	LOC124900255	NNNAGGCAATGACACAAAGT	NGA	2	0.0556	Tier III
28	NC_000007.14	7	96408837	+	LOC105375410	NNNAGGCTATGAGAAAAAGT	NGG	2	0.0303	Tier III

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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000069.6	3	122830271	-	Pde5a	NNNAGTCTAAGACACAAAGT	NGG	2	0.5714	Tier II
2	NC_000085.6	19	46264205	-	Gbf1	NNNAGGCAATGATACAAAGT	NGG	2	0.56	Tier II
3	NC_000082.6	16	52352219	-	Alcam	NNNAGGCTAAGACTCAAAGT	NGG	2	0.4571	Tier II
4	NC_000074.6	8	80831512	-	Gab1	NNNAGGATCTGACACAAAGT	NGG	2	0.4286	Tier II
5	NC_000068.7	2	38606085	+	Psmb7	NNNAGGCAATGACACAATGT	NGG	2	0.4	Tier II
6	NC_000076.6	10	43892044	-	Qrsl1	NNNAGGTTATGACACATAGT	NGG	2	0.1083	Tier II
7	NC_000071.6	5	66478490	+	Apbb2	NNNAGGCTATGACTGAAAGT	NGG	2	0.0267	Tier II
8	NC_000079.6	13	29955514	+	E2f3	NNNAGGCTATGAAACAAAGT	NGT	2	0.0062	Tier II
9	NC_000076.6	10	99776505	+	Gm35206	NNNAGGCCATGGCACAAAGT	NGG	2	0.7222	Tier III
10	NC_000069.6	3	122830271	-	Gm34577	NNNAGTCTAAGACACAAAGT	NGG	2	0.5714	Tier III
11	NC_000082.6	16	52352219	-	Gm41461	NNNAGGCTAAGACTCAAAGT	NGG	2	0.4571	Tier III
12	NC_000072.6	6	12102565	-	Gm6578	NNNAGCCTATGACACTAAGT	NGG	2	0.0	Tier III
13	NC_000076.6	10	61031913	+	Gm20611	NNNAGGCTATGACACCAAAT	NGG	2	0.0	Tier III
14	NC_000071.6	5	28010032	+	Gm35223	NNNAGGCTATGACAGCAAGT	NGG	2	0.0	Tier III

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Other clones with same target sequence:

(none)