Construct: sgRNA BRDN0001147268
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGAGTTTGAGCGACGGGTA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- AK1 (203)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | NC_000009.12 | 9 | 127871823 | - | AK1 | NNNAGTTTGAGCGACGGGTA | NGG | 0 | 1.0 | Tier I |
| 2 | NC_000008.11 | 8 | 123230204 | - | C8orf76 | NNNAGTTTGAGGGAAGGGTA | NGG | 2 | 0.0988 | Tier II |
| 3 | NC_000008.11 | 8 | 123230204 | - | ZHX1-C8orf76 | NNNAGTTTGAGGGAAGGGTA | NGG | 2 | 0.0988 | Tier II |
| 4 | NC_000009.12 | 9 | 127871823 | - | ST6GALNAC4-ST6GALNAC6-AK1 | NNNAGTTTGAGCGACGGGTA | NGG | 0 | 1.0 | Tier III |
| 5 | NC_000016.10 | 16 | 79601259 | - | LOC101928230 | NNNAGTTTGAGCGCCGGGCA | NGG | 2 | 0.0591 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | NC_000075.6 | 9 | 48960407 | - | Htr3b | NNNAGTTTGAGCTAAGGGTA | NGG | 2 | 0.0667 | Tier II |