Construct: sgRNA BRDN0001147279
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATATGGCGAGGTTCTACAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDC42BPB (9578)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77779
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
102999622 |
- |
CDC42BPB |
NNNATGGCGAGGTTCTACAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
44129137 |
- |
JPH2 |
NNNATGGAGAGGTTCTACAT |
NGG |
1 |
0.65 |
Tier II |
| 3 |
NC_000007.14 |
7 |
72914201 |
+ |
POM121 |
NNNATGGACAGGTTCTACAT |
NGG |
2 |
0.35 |
Tier II |
| 4 |
NC_000001.11 |
1 |
154770185 |
+ |
KCNN3 |
NNNATGGCGAGGTTCTGAAT |
NGG |
2 |
0.095 |
Tier II |
| 5 |
NC_000004.12 |
4 |
181564553 |
- |
TENM3 |
NNNATGGCGAGCTTGTACAT |
NGG |
2 |
0.0265 |
Tier II |
| 6 |
NC_000012.12 |
12 |
131245725 |
- |
LOC105370082 |
NNNATGGCGAGGGTCTACAT |
NAG |
2 |
0.0676 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
111336083 |
- |
Cdc42bpb |
NNNATGGCGAGGTTCTACAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000076.6 |
10 |
33475018 |
+ |
Trdn |
NNNATGGCAAGGATCTACAT |
NGG |
2 |
0.4451 |
Tier II |
| 3 |
NC_000078.6 |
12 |
108318869 |
- |
Hhipl1 |
NNNATGTCGAGGGTCTACAT |
NGG |
2 |
0.1491 |
Tier II |
| 4 |
NC_000071.6 |
5 |
21761371 |
+ |
Dnajc2 |
NNNATGTCGAGGTGCTACAT |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000071.6 |
5 |
63624141 |
- |
Gm30837 |
NNNATGGCTAGATTCTACAT |
NGG |
2 |
0.4978 |
Tier III |
Other clones with same target sequence:
(none)
