Construct: sgRNA BRDN0001147285

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAAGAGAATTGAGTGCTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PFKFB1 (5207)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77630

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 54956193 - PFKFB1 NNNGAGAATTGAGTGCTATG NGG 0 1.0 Tier I
2 NC_000017.11 17 76015441 - EVPL NNNGAGAAGTGAGTGCTCTG NGG 2 0.1179 Tier I
3 NC_000018.10 18 57472859 + ONECUT2 NNNGAAAATTGAGAGCTATG NGG 2 0.619 Tier II
4 NC_000002.12 2 200890050 + NIF3L1 NNNGAGAATGGAGTACTATG NGG 2 0.4706 Tier II
5 NC_000023.11 X 132955735 - HS6ST2 NNNGGGAAGTGAGTGCTATG NGG 2 0.4457 Tier II
6 NC_000013.11 13 72800857 + PIBF1 NNNGTGAATTGAGTGCTATA NGG 2 0.3409 Tier II
7 NC_000005.10 5 65676647 - SGTB NNNGTGAATTGAGTGCCATG NGG 2 0.2567 Tier II
8 NC_000006.12 6 89593205 - ANKRD6 NNNGAGAAGTGTGTGCTATG NGG 2 0.2063 Tier II
9 NC_000004.12 4 36097679 - ARAP2 NNNGAGAATTTAGTGCTTTG NGG 2 0.1923 Tier II
10 NC_000011.10 11 64718499 + NRXN2 NNNGAGAAGTGCGTGCTATG NGG 2 0.1629 Tier II
11 NC_000001.11 1 185282799 + SWT1 NNNGAGAATTGCGTGCTGTG NGG 2 0.1053 Tier II
12 NC_000012.12 12 13750822 + GRIN2B NNNGAGAAATGAGTGCTATG NGA 2 0.0645 Tier II
13 NC_000008.11 8 72668147 - KCNB2 NNNGAGAATTAAGTGCTATG NGT 2 0.0161 Tier II
14 NC_000018.10 18 23336119 - TMEM241 NNNGAGAAATGAGTGCTATG NGT 2 0.015 Tier II
15 NC_000003.12 3 105857868 + CBLB NNNGAGAATTTAGTGCTATG NTG 2 0.015 Tier II
16 NC_000013.11 13 106261162 - LOC107984626 NNNGAGAAATGGGTGCTATG NGG 2 0.6706 Tier III
17 NC_000003.12 3 117913724 - LINC03051 NNNAAGAATTTAGTGCTATG NGG 2 0.3462 Tier III
18 NC_000001.11 1 185282799 + LOC105371651 NNNGAGAATTGCGTGCTGTG NGG 2 0.1053 Tier III
19 NC_000006.12 6 133551351 + TARID NNNGAGAAGTGAGTGGTATG NGG 2 0.0952 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 130705420 - Pfkfb2 NNNGAGAATTGAGTGCTACA NGG 2 0.2586 Tier I
2 NC_000086.7 X 150622164 + Pfkfb1 NNNGAGAATTGAGTGCTATG NGA 1 0.0694 Tier I
3 NC_000072.6 6 85342101 + Rab11fip5 NNNGAGAATTTAGTGCTATG NCG 2 0.0412 Tier I
4 NC_000071.6 5 92239488 + Ppef2 NNNGAAAATTGAGTGCTATG NGG 1 1.0 Tier II
5 NC_000069.6 3 156584166 - Negr1 NNNGAAAGTTGAGTGCTATG NGG 2 0.7333 Tier II
6 NC_000067.6 1 85096226 - A530032D15Rik NNNGGGAAATGAGTGCTATG NGG 2 0.6686 Tier II
7 NC_000067.6 1 85162046 + Gm38510 NNNGGGAAATGAGTGCTATG NGG 2 0.6686 Tier II
8 NC_000067.6 1 85256445 - C130026I21Rik NNNGGGAAATGAGTGCTATG NGG 2 0.6686 Tier II
9 NC_000067.6 1 85256445 - Gm38510 NNNGGGAAATGAGTGCTATG NGG 2 0.6686 Tier II
10 NC_000067.6 1 85489393 + Gm7592 NNNGGGAAATGAGTGCTATG NGG 2 0.6686 Tier II
11 NC_000067.6 1 85612896 + Sp140 NNNGGGAAATGAGTGCTATG NGG 2 0.6686 Tier II
12 NT_162750.1 1 118294 + Csprs NNNGGGAAATGAGTGCTATG NGG 2 0.6686 Tier II
13 NT_166280.1 1 13969 + LOC100041708 NNNGGGAAATGAGTGCTATG NGG 2 0.6686 Tier II
14 NT_166280.1 1 128203 + LOC101055672 NNNGGGAAATGAGTGCTATG NGG 2 0.6686 Tier II
15 NT_166281.1 1 180352 + LOC100041057 NNNGGGAAATGAGTGCTATG NGG 2 0.6686 Tier II
16 NT_166282.1 1 90921 - LOC100041903 NNNGGGAAATGAGTGCTATG NGG 2 0.6686 Tier II
17 NC_000084.6 18 25348486 - AW554918 NNNGATAATTAAGTGCTATG NGG 2 0.6667 Tier II
18 NC_000067.6 1 100043664 + Cntnap5b NNNGAGAATTAAGAGCTATG NGG 2 0.619 Tier II
19 NC_000069.6 3 89088584 + Rusc1 NNNGAGAATTGAAAGCTATG NGG 2 0.5714 Tier II
20 NC_000069.6 3 54749471 + Alg5 NNNGAGAATTAACTGCTATG NGG 2 0.4211 Tier II
21 NC_000075.6 9 75399032 - Mapk6 NNNGAGGATTGAGTGCTGTG NGG 2 0.2824 Tier II
22 NC_000073.6 7 64552308 + Apba2 NNNGAGAAATGAGTCCTATG NGG 2 0.2532 Tier II
23 NC_000068.7 2 122308287 - Duoxa1 NNNGAGAATGGAGTGCTGTG NGG 2 0.2 Tier II
24 NC_000077.6 11 61169405 - Usp22 NNNGAGTATTCAGTGCTATG NGG 2 0.1875 Tier II
25 NC_000080.6 14 4083360 + Gm32966 NNNGAGAAGTGATTGCTATG NGG 2 0.1857 Tier II
26 NC_000080.6 14 4837302 + Gm26650 NNNGAGAAGTGATTGCTATG NGG 2 0.1857 Tier II
27 NC_000076.6 10 122862758 + Ppm1h NNNTAGAATTGAGTGCTTTG NGG 2 0.1818 Tier II
28 NC_000083.6 17 17829973 + Spaca6 NNNGAGAATTGAGTGCAAGG NGG 2 0.1333 Tier II
29 NC_000086.7 X 36352668 - Lonrf3 NNNGAGAATTGATTGCTGTG NGG 2 0.12 Tier II
30 NC_000069.6 3 64095016 - Vmn2r1 NNNGAGATTTGAGTGCTCTG NGG 2 0.0816 Tier II
31 NC_000076.6 10 128677739 + Rab5b NNNGAGAATTGAGTCCTAAG NGG 2 0.0779 Tier II
32 NC_000074.6 8 61273264 + Sh3rf1 NNNGAGTATTGAGTTCTATG NGG 2 0.0625 Tier II
33 NC_000072.6 6 99210560 - Foxp1 NNNGAGAATTGAGTGCTGTG NTG 2 0.0156 Tier II
34 NC_000071.6 5 31715781 + Babam2 NNNGAGAATTTAGTGCTATG NGT 2 0.0062 Tier II
35 NC_000067.6 1 85489393 + Gm2666 NNNGGGAAATGAGTGCTATG NGG 2 0.6686 Tier III
36 NT_162750.1 1 50903 + LOC115489307 NNNGGGAAATGAGTGCTATG NGG 2 0.6686 Tier III
37 NT_162750.1 1 50903 + LOC115489308 NNNGGGAAATGAGTGCTATG NGG 2 0.6686 Tier III
38 NT_162750.1 1 118294 + LOC105242429 NNNGGGAAATGAGTGCTATG NGG 2 0.6686 Tier III
39 NT_166456.1 23207 + LOC673191 NNNGGGAAATGAGTGCTATG NGG 2 0.6686 Tier III
40 NC_000080.6 14 6059014 - Gm51421 NNNGAGAAGTGATTGCTATG NGG 2 0.1857 Tier III
41 NC_000080.6 14 6445358 - Gm26630 NNNGAGAAGTGATTGCTATG NGG 2 0.1857 Tier III
42 NC_000067.6 1 72121304 - Gm4319 NNNGAGATTTGAGTGCTCTG NGG 2 0.0816 Tier III
43 NC_000068.7 2 116526049 + Gm13991 NNNGAGAATTGAGTCCTAAG NGG 2 0.0779 Tier III
44 NC_000072.6 6 99210560 - Gm52870 NNNGAGAATTGAGTGCTGTG NTG 2 0.0156 Tier III
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Other clones with same target sequence:

(none)