Construct: sgRNA BRDN0001147288
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGCCCATGACGAGTAACCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ULK2 (9706)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77262
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
19796225 |
+ |
ULK2 |
NNNCCCATGACGAGTAACCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
57610903 |
+ |
GCOM1 |
NNNCCCATGAAGAGAAACCA |
NGG |
2 |
0.4342 |
Tier II |
| 3 |
NC_000015.10 |
15 |
57610903 |
+ |
MYZAP |
NNNCCCATGAAGAGAAACCA |
NGG |
2 |
0.4342 |
Tier II |
| 4 |
NC_000015.10 |
15 |
67806787 |
+ |
MAP2K5 |
NNNCCCAGGACCAGTAACCA |
NGG |
2 |
0.3882 |
Tier II |
| 5 |
NC_000016.10 |
16 |
81631065 |
+ |
CMIP |
NNNCCCATAACTAGTAACCA |
NGG |
2 |
0.2473 |
Tier II |
| 6 |
NC_000004.12 |
4 |
49038501 |
- |
CWH43 |
NNNCCCATGGCGAGGAACCA |
NGG |
2 |
0.0278 |
Tier II |
| 7 |
NC_000016.10 |
16 |
81631065 |
+ |
LOC105371362 |
NNNCCCATAACTAGTAACCA |
NGG |
2 |
0.2473 |
Tier III |
| 8 |
NC_000023.11 |
X |
38991431 |
+ |
LOC124905177 |
NNNCCCATGAAGAGGAACCA |
NGG |
2 |
0.0375 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
61799593 |
+ |
Ulk2 |
NNNCCCATGACGAGTAACCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000080.6 |
14 |
22056789 |
+ |
Lrmda |
NNNCCCATCACCAGTAACCA |
NGG |
2 |
0.2851 |
Tier II |
| 3 |
NC_000085.6 |
19 |
43926820 |
- |
Dnmbp |
NNNCCCATGACCAGTAACCA |
NAG |
2 |
0.1373 |
Tier II |
| 4 |
NC_000071.6 |
5 |
98398468 |
- |
Cfap299 |
NNNCCCATGACGAGTAACAA |
NGA |
2 |
0.0298 |
Tier II |
| 5 |
NC_000080.6 |
14 |
22056789 |
+ |
Gm41107 |
NNNCCCATCACCAGTAACCA |
NGG |
2 |
0.2851 |
Tier III |
| 6 |
NC_000075.6 |
9 |
58467422 |
- |
4930461G14Rik |
NNNCCCATGGCTAGTAACCA |
NGG |
2 |
0.2137 |
Tier III |
| 7 |
NC_000075.6 |
9 |
58467422 |
- |
Gm32768 |
NNNCCCATGGCTAGTAACCA |
NGG |
2 |
0.2137 |
Tier III |
| 8 |
NC_000085.6 |
19 |
43926820 |
- |
Gm50217 |
NNNCCCATGACCAGTAACCA |
NAG |
2 |
0.1373 |
Tier III |
Other clones with same target sequence:
(none)
