Construct: sgRNA BRDN0001147293
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGCTATCAAATGGACCCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SNX16 (64089)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75836
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
81815355 |
+ |
SNX16 |
NNNCTATCAAATGGACCCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
22984085 |
- |
ARMC3 |
NNNCTATCAAATGCACCTGG |
NGG |
2 |
0.2755 |
Tier II |
| 3 |
NC_000008.11 |
8 |
62214354 |
- |
LOC124901952 |
NNNCTATCGAATGGACCCAG |
NGG |
2 |
0.4762 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
10426193 |
+ |
Snx16 |
NNNCTATCAAATGGACCTGG |
NGG |
1 |
0.6429 |
Tier I |
| 2 |
NC_000068.7 |
2 |
26228808 |
+ |
Qsox2 |
NNNCAATCAGATGGACCCGG |
NGG |
2 |
0.2778 |
Tier II |
| 3 |
NC_000076.6 |
10 |
123265663 |
+ |
Tafa2 |
NNNCTATCAACTGGGCCCGG |
NGG |
2 |
0.26 |
Tier II |
| 4 |
NC_000077.6 |
11 |
95983979 |
- |
Igf2bp1 |
NNNCTATAAAATGGACCCGG |
NAG |
2 |
0.1685 |
Tier II |
Other clones with same target sequence:
(none)
