Construct: sgRNA BRDN0001147297

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTCCCAAACTGACCTCTAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DGKA (1606)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76605

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 55940430 - DGKA NNNCCAAACTGACCTCTAGG NGG 0 1.0 Tier I
2 NC_000005.10 5 149152557 - ABLIM3 NNNCAAAACTGACCTCCAGG NGG 2 0.4034 Tier II
3 NC_000019.10 19 22237516 + ZNF676 NNNTCAAACTCACCTCTAGG NGG 2 0.3429 Tier II
4 NC_000015.10 15 58056189 - ALDH1A2 NNNACAAACTTACCTCTAGG NGG 2 0.3239 Tier II
5 NC_000002.12 2 212128309 - ERBB4 NNNCCAAACTTACCTCTAAG NGG 2 0.2747 Tier II
6 NC_000023.11 X 53311551 + IQSEC2 NNNCCAAACTGCCCTCTAGG NGG 1 0.2632 Tier II
7 NC_000009.12 9 127310896 - GARNL3 NNNCCAATCTGACATCTAGG NGG 2 0.15 Tier II
8 NC_000012.12 12 132305731 - GALNT9 NNNCCAAACTGAGCTCCAGG NGG 2 0.0963 Tier II
9 NC_000023.11 X 33165206 - DMD NNNCCAAACTGACATGTAGG NGG 2 0.0538 Tier II
10 NC_000002.12 2 233981540 - TRPM8 NNNCCAAACTGAGCTCTCGG NGG 2 0.026 Tier II
11 NC_000014.9 14 73326265 + NUMB NNNCCAAACTGACCCCTAGG NGA 2 0.0189 Tier II
12 NC_000009.12 9 123363445 - CRB2 NNNCCAAACTGAACTCTAGG NTG 2 0.015 Tier II
13 NC_000015.10 15 25220269 - SNHG14 NNNCCAAACTGACCTCCATG NGG 2 0.4706 Tier III
14 NC_000015.10 15 25215314 - SNHG14 NNNCCAAACTGACCTCTACG NGG 1 0.4483 Tier III
15 NC_000011.10 11 39724594 - LOC105376637 NNNCCAAAGCGACCTCTAGG NGG 2 0.3302 Tier III
16 NC_000012.12 12 75711320 - LOC105369844 NNNTCAAACTGACCTCTAGG NAG 2 0.2074 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000085.6 19 21792803 + Cemip2 NNNCCAAACTGACCTCCAGG NGC 2 0.0157 Tier I
2 NC_000071.6 5 21265231 + Gsap NNNCCAAACTGACCACTAGA NGG 2 0.5428 Tier II
3 NC_000083.6 17 63237307 + Fbxl17 NNNAGAAACTGACCTCTAGG NGG 2 0.5053 Tier II
4 NC_000084.6 18 44393632 - Dcp2 NNNCCAAACTGGCCACTAGG NGG 2 0.4181 Tier II
5 NC_000067.6 1 153684822 + Rgs8 NNNCCAGCCTGACCTCTAGG NGG 2 0.3025 Tier II
6 NC_000068.7 2 126049827 + Fgf7 NNNCCAAACTGAACTCTAAG NGG 2 0.2747 Tier II
7 NC_000068.7 2 126049827 + Fam227b NNNCCAAACTGAACTCTAAG NGG 2 0.2747 Tier II
8 NC_000078.6 12 75492498 + Ppp2r5e NNNCCAAACTTACCTCTAAG NGG 2 0.2747 Tier II
9 NC_000085.6 19 42332572 + Crtac1 NNNCCAACCTCACCTCTAGG NGG 2 0.1837 Tier II
10 NC_000077.6 11 119970815 + Baiap2 NNNGCAAACTGACCTCTAGG NAG 2 0.1296 Tier II
11 NC_000069.6 3 69348615 - Ppm1l NNNCCAAACTGCCCTGTAGG NGG 2 0.0405 Tier II
12 NC_000077.6 11 79625079 - Rab11fip4 NNNCCACACTGACCTCTAGG NGA 2 0.0304 Tier II
13 NC_000075.6 9 110431245 + Klhl18 NNNCCAAACTGAGCGCTAGG NGG 2 0.0068 Tier II
14 NC_000070.6 4 44176318 + Rnf38 NNNCCAAACTGACCTCTAGG NAA 2 0.0 Tier II
15 NC_000083.6 17 83101035 + Gm46606 NNNCCAGAGTGACCTCTAGG NGG 2 0.437 Tier III
16 NC_000075.6 9 120070520 + Gm33858 NNNCCACACTGACCTCTAGA NGG 2 0.4102 Tier III
17 NC_000078.6 12 99612781 - Gm35644 NNNCCAAACTGTCCTCGAGG NGG 2 0.0392 Tier III
18 NC_000078.6 12 99612781 - Gm26839 NNNCCAAACTGTCCTCGAGG NGG 2 0.0392 Tier III
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Other clones with same target sequence:

(none)