Construct: sgRNA BRDN0001147302
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTGGGCCTCTCACCCAACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHB6 (2051)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77274
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
142864421 |
+ |
EPHB6 |
NNNGGGCCTCTCACCCAACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000021.9 |
21 |
45984467 |
- |
COL6A1 |
NNNGGGCCACTCACCCCACG |
NGG |
2 |
0.1639 |
Tier I |
| 3 |
NC_000023.11 |
X |
152921000 |
+ |
ZNF185 |
NNNGGGCCCCTGACCCAACG |
NGG |
2 |
0.4103 |
Tier II |
| 4 |
NC_000011.10 |
11 |
117592015 |
- |
DSCAML1 |
NNNGGGCCTGTCACCCAACC |
NGG |
2 |
0.1667 |
Tier II |
| 5 |
NC_000015.10 |
15 |
77671561 |
- |
LINGO1 |
NNNGGGCCTCTCTCCCAAAG |
NGG |
2 |
0.1286 |
Tier II |
| 6 |
NC_000015.10 |
15 |
89644979 |
- |
KIF7 |
NNNGGGCCTCTCACCCGAGG |
NGG |
2 |
0.0221 |
Tier II |
| 7 |
NC_000002.12 |
2 |
240922974 |
+ |
CROCC2 |
NNNGGGCCTCTCACGCAACC |
NGG |
2 |
0.0214 |
Tier II |
| 8 |
NC_000008.11 |
8 |
41282174 |
+ |
SFRP1 |
NNNGGGCCTCTCACCCAAGG |
NGT |
2 |
0.002 |
Tier II |
| 9 |
NC_000010.11 |
10 |
21752655 |
- |
LOC107984214 |
NNNGGGCCTCTCACCCAAAA |
NGG |
2 |
0.4018 |
Tier III |
| 10 |
NC_000007.14 |
7 |
63699348 |
- |
LOC105375313 |
NNNGGGCCCCTCACCCCACG |
NGG |
2 |
0.1629 |
Tier III |
| 11 |
NC_000002.12 |
2 |
111380505 |
- |
MIR4435-2HG |
NNNTGGCCTCTCACCCAACG |
NTG |
2 |
0.0142 |
Tier III |
| 12 |
NC_000019.10 |
19 |
48659953 |
- |
SEC1P |
NNNGGGCCTCTCAGCCAATG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
80638930 |
- |
Nup35 |
NNNGGTCCACTCACCCAACG |
NGG |
2 |
0.619 |
Tier I |
| 2 |
NC_000083.6 |
17 |
8190657 |
- |
Cep43 |
NNNGGGCCTATCACCCAAAG |
NGG |
2 |
0.3714 |
Tier II |
| 3 |
NC_000078.6 |
12 |
76014247 |
+ |
Syne2 |
NNNGGGCCTCTTACCCAACC |
NGG |
2 |
0.2308 |
Tier II |
| 4 |
NC_000072.6 |
6 |
85424787 |
+ |
Noto |
NNNGGGCCTCTCACCTAACC |
NGG |
2 |
0.1319 |
Tier II |
| 5 |
NC_000070.6 |
4 |
155544553 |
- |
Gnb1 |
NNNGGGCCTCTCACTGAACG |
NGG |
2 |
0.0103 |
Tier II |
| 6 |
NC_000075.6 |
9 |
113727706 |
- |
Gm38623 |
NNNTGGCCTCTCACCCAAAG |
NGG |
2 |
0.1558 |
Tier III |
Other clones with same target sequence:
(none)
