Construct: sgRNA BRDN0001147303
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAAATGGGCATATTGCCACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIKFYVE (200576)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76892
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
208301090 |
+ |
PIKFYVE |
NNNATGGGCATATTGCCACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
91765674 |
- |
ROR2 |
NNNATGAGCACATTGCCACA |
NGG |
2 |
0.6667 |
Tier II |
| 3 |
NC_000012.12 |
12 |
59788439 |
+ |
SLC16A7 |
NNNATGATCATATTGCCACA |
NGG |
2 |
0.625 |
Tier II |
| 4 |
NC_000001.11 |
1 |
232501955 |
- |
SIPA1L2 |
NNNATGGGCATAATGCCACA |
NAG |
2 |
0.1795 |
Tier II |
| 5 |
NC_000009.12 |
9 |
27506013 |
+ |
MOB3B |
NNNCTGGGCAGATTGCCACA |
NGG |
2 |
0.1412 |
Tier II |
| 6 |
NC_000011.10 |
11 |
96248961 |
+ |
MAML2 |
NNNATGGGAATATTTCCACA |
NGG |
2 |
0.1224 |
Tier II |
| 7 |
NC_000007.14 |
7 |
92612301 |
- |
CDK6 |
NNNAAGGGCATATTGCCCCA |
NGG |
2 |
0.0952 |
Tier II |
| 8 |
NC_000002.12 |
2 |
195928019 |
+ |
DNAH7 |
NNNATGGGCATATTGCACCA |
NGG |
2 |
0.0889 |
Tier II |
| 9 |
NC_000002.12 |
2 |
241649629 |
+ |
ATG4B |
NNNATGGGCATCTCGCCACA |
NGG |
2 |
0.0752 |
Tier II |
| 10 |
NC_000018.10 |
18 |
26979113 |
- |
CHST9 |
NNNATGGGCATATTTCCAAA |
NGG |
2 |
0.0612 |
Tier II |
| 11 |
NC_000015.10 |
15 |
49215952 |
+ |
GALK2 |
NNNATGGGCATATCGGCACA |
NGG |
2 |
0.044 |
Tier II |
| 12 |
NC_000004.12 |
4 |
26934782 |
+ |
STIM2 |
NNNATGGGCATATTGGCACA |
NGC |
2 |
0.0034 |
Tier II |
| 13 |
NC_000019.10 |
19 |
13867851 |
- |
NANOS3 |
NNNATGGGCATATGGCCACT |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000011.10 |
11 |
106972004 |
+ |
GUCY1A2 |
NNNATGGGCATATTGCCACA |
NAC |
2 |
0.0 |
Tier II |
| 15 |
NC_000018.10 |
18 |
64419356 |
+ |
LINC01924 |
NNNTTGGGGATATTGCCACA |
NGG |
2 |
0.3939 |
Tier III |
| 16 |
NC_000018.10 |
18 |
26979113 |
- |
LOC124904275 |
NNNATGGGCATATTTCCAAA |
NGG |
2 |
0.0612 |
Tier III |
| 17 |
NC_000012.12 |
12 |
8185843 |
- |
FAM66C |
NNNATGGGAATATTGCCACA |
NGC |
2 |
0.019 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
110776276 |
- |
Pus1 |
NNNATAGGCATATTGCCACA |
NGG |
1 |
1.0 |
Tier II |
| 2 |
NC_000067.6 |
1 |
32642136 |
- |
Khdrbs2 |
NNNATGGGCATAATGCAACA |
NGG |
2 |
0.3231 |
Tier II |
| 3 |
NC_000077.6 |
11 |
85448028 |
+ |
Bcas3 |
NNNATGAGCATATTGGCACA |
NGG |
2 |
0.1538 |
Tier II |
| 4 |
NC_000082.6 |
16 |
57421798 |
+ |
Cmss1 |
NNNATGGGCAGATTCCCACA |
NGG |
2 |
0.1091 |
Tier II |
| 5 |
NC_000082.6 |
16 |
57421798 |
+ |
Filip1l |
NNNATGGGCAGATTCCCACA |
NGG |
2 |
0.1091 |
Tier II |
| 6 |
NC_000077.6 |
11 |
101180214 |
+ |
Cntnap1 |
NNNATGGGCATATTTGCACA |
NGG |
2 |
0.022 |
Tier II |
| 7 |
NC_000069.6 |
3 |
38938352 |
+ |
Fat4 |
NNNATGGGCATATTGCCACA |
NGT |
1 |
0.0161 |
Tier II |
| 8 |
NC_000071.6 |
5 |
24829254 |
+ |
Rheb |
NNNATGGGCATATTGCCTCA |
NGT |
2 |
0.0081 |
Tier II |
| 9 |
NC_000073.6 |
7 |
82775456 |
- |
Efl1 |
NNNATGGGCACATGGCCACA |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000072.6 |
6 |
90321111 |
+ |
Chst13 |
NNNATGGGCATATGTCCACA |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000071.6 |
5 |
110776276 |
- |
Gm15559 |
NNNATAGGCATATTGCCACA |
NGG |
1 |
1.0 |
Tier III |
| 12 |
NC_000078.6 |
12 |
16005770 |
- |
Gm36235 |
NNNACGGGCAAATTGCCACA |
NGG |
2 |
0.75 |
Tier III |
| 13 |
NC_000078.6 |
12 |
107730156 |
- |
4930465M20Rik |
NNNTTGGGCATATTGCCACA |
NGG |
1 |
0.6364 |
Tier III |
| 14 |
NC_000085.6 |
19 |
50703974 |
+ |
Gm26629 |
NNNATGGGAATATTGCCACA |
NGT |
2 |
0.0138 |
Tier III |
Other clones with same target sequence:
(none)
