Construct: sgRNA BRDN0001147306
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGGATGATTGAGTCTAACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DLG3 (1741)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76156
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
70492592 |
+ |
DLG3 |
NNNGATGATTGAGTCTAACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
55665981 |
- |
OR4C6 |
NNNGATGATTGAGTCTGACA |
NAG |
2 |
0.0458 |
Tier I |
| 3 |
NC_000005.10 |
5 |
149759943 |
- |
PPARGC1B |
NNNGGTGAATGAGTCTAACA |
NGG |
2 |
0.6686 |
Tier II |
| 4 |
NC_000006.12 |
6 |
167312026 |
- |
UNC93A |
NNNGATGACTGAGTCCAACA |
NGG |
2 |
0.6154 |
Tier II |
| 5 |
NC_000010.11 |
10 |
18399152 |
+ |
CACNB2 |
NNNGGTGATTGGGTCTAACA |
NGG |
2 |
0.52 |
Tier II |
| 6 |
NC_000022.11 |
22 |
40307558 |
- |
TNRC6B |
NNNGATGACGGAGTCTAACA |
NGG |
2 |
0.4615 |
Tier II |
| 7 |
NC_000003.12 |
3 |
53344649 |
- |
DCP1A |
NNNAATGATTGAGTCTAAAA |
NGG |
2 |
0.3857 |
Tier II |
| 8 |
NC_000002.12 |
2 |
162631392 |
+ |
KCNH7 |
NNNGATGATTGTGTGTAACA |
NGG |
2 |
0.0167 |
Tier II |
| 9 |
NC_000015.10 |
15 |
80506459 |
- |
ARNT2 |
NNNGATGATTGAGTCTAACC |
NGA |
2 |
0.0158 |
Tier II |
| 10 |
NC_000003.12 |
3 |
191570910 |
- |
PYDC2-AS1 |
NNNAATGAATGAGTCTAACA |
NGG |
2 |
0.8357 |
Tier III |
| 11 |
NC_000003.12 |
3 |
164063227 |
+ |
LOC102724419 |
NNNGATGATTGAATCTAACA |
NTG |
2 |
0.036 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
3634958 |
+ |
Gucy1a2 |
NNNGATGTTTGACTCTAACA |
NGG |
2 |
0.1805 |
Tier I |
| 2 |
NC_000086.7 |
X |
100807251 |
+ |
Dlg3 |
NNNGATGATTGAGTCTAATC |
NGG |
2 |
0.1049 |
Tier I |
| 3 |
NC_000085.6 |
19 |
32803272 |
- |
Pten |
NNNGATGAATGAGTCAAACA |
NGG |
2 |
0.8442 |
Tier II |
| 4 |
NC_000067.6 |
1 |
188783583 |
+ |
Ush2a |
NNNGATGACTGAGTCCAACA |
NGG |
2 |
0.6154 |
Tier II |
| 5 |
NC_000067.6 |
1 |
139555423 |
+ |
Cfhr1 |
NNNGAGCATTGAGTCTAACA |
NGG |
2 |
0.3929 |
Tier II |
| 6 |
NC_000070.6 |
4 |
104131884 |
- |
Dab1 |
NNNGATTCTTGAGTCTAACA |
NGG |
2 |
0.2449 |
Tier II |
| 7 |
NC_000077.6 |
11 |
81570361 |
- |
Asic2 |
NNNGATCATTGAGTCTAACA |
NAG |
2 |
0.1782 |
Tier II |
| 8 |
NC_000080.6 |
14 |
122903913 |
- |
Ggact |
NNNCATGATTGAGTTTAACA |
NGG |
2 |
0.0353 |
Tier II |
| 9 |
NC_000071.6 |
5 |
51646827 |
- |
Ppargc1a |
NNNAATGATTGAGTCTAACA |
NTG |
2 |
0.0351 |
Tier II |
| 10 |
NC_000067.6 |
1 |
110033361 |
- |
Cdh7 |
NNNGATGATTGAGAGTAACA |
NGG |
2 |
0.031 |
Tier II |
| 11 |
NC_000079.6 |
13 |
23361202 |
- |
Zfp322a |
NNNGAGGATTGAGTGTAACA |
NGG |
2 |
0.0286 |
Tier II |
| 12 |
NC_000067.6 |
1 |
70706483 |
+ |
Spag16 |
NNNGATTATTGAGTGTAACA |
NGG |
2 |
0.0286 |
Tier II |
| 13 |
NC_000068.7 |
2 |
11427857 |
+ |
Gm31990 |
NNNGATGAGTGAGTCTAACA |
NGG |
1 |
0.619 |
Tier III |
| 14 |
NC_000070.6 |
4 |
78345005 |
+ |
Gm32545 |
NNNGATGATTGAATCTAACA |
NAG |
2 |
0.2393 |
Tier III |
| 15 |
NC_000086.7 |
X |
105162336 |
- |
Gm33605 |
NNNGATGCTTGAGTCTCACA |
NGG |
2 |
0.0756 |
Tier III |
| 16 |
NC_000079.6 |
13 |
23361202 |
- |
4930586N03Rik |
NNNGAGGATTGAGTGTAACA |
NGG |
2 |
0.0286 |
Tier III |
Other clones with same target sequence:
(none)
