Construct: sgRNA BRDN0001147306

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGGGATGATTGAGTCTAACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DLG3 (1741)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76156

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 70492592 + DLG3 NNNGATGATTGAGTCTAACA NGG 0 1.0 Tier I
2 NC_000011.10 11 55665981 - OR4C6 NNNGATGATTGAGTCTGACA NAG 2 0.0458 Tier I
3 NC_000005.10 5 149759943 - PPARGC1B NNNGGTGAATGAGTCTAACA NGG 2 0.6686 Tier II
4 NC_000006.12 6 167312026 - UNC93A NNNGATGACTGAGTCCAACA NGG 2 0.6154 Tier II
5 NC_000010.11 10 18399152 + CACNB2 NNNGGTGATTGGGTCTAACA NGG 2 0.52 Tier II
6 NC_000022.11 22 40307558 - TNRC6B NNNGATGACGGAGTCTAACA NGG 2 0.4615 Tier II
7 NC_000003.12 3 53344649 - DCP1A NNNAATGATTGAGTCTAAAA NGG 2 0.3857 Tier II
8 NC_000002.12 2 162631392 + KCNH7 NNNGATGATTGTGTGTAACA NGG 2 0.0167 Tier II
9 NC_000015.10 15 80506459 - ARNT2 NNNGATGATTGAGTCTAACC NGA 2 0.0158 Tier II
10 NC_000003.12 3 191570910 - PYDC2-AS1 NNNAATGAATGAGTCTAACA NGG 2 0.8357 Tier III
11 NC_000003.12 3 164063227 + LOC102724419 NNNGATGATTGAATCTAACA NTG 2 0.036 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 3634958 + Gucy1a2 NNNGATGTTTGACTCTAACA NGG 2 0.1805 Tier I
2 NC_000086.7 X 100807251 + Dlg3 NNNGATGATTGAGTCTAATC NGG 2 0.1049 Tier I
3 NC_000085.6 19 32803272 - Pten NNNGATGAATGAGTCAAACA NGG 2 0.8442 Tier II
4 NC_000067.6 1 188783583 + Ush2a NNNGATGACTGAGTCCAACA NGG 2 0.6154 Tier II
5 NC_000067.6 1 139555423 + Cfhr1 NNNGAGCATTGAGTCTAACA NGG 2 0.3929 Tier II
6 NC_000070.6 4 104131884 - Dab1 NNNGATTCTTGAGTCTAACA NGG 2 0.2449 Tier II
7 NC_000077.6 11 81570361 - Asic2 NNNGATCATTGAGTCTAACA NAG 2 0.1782 Tier II
8 NC_000080.6 14 122903913 - Ggact NNNCATGATTGAGTTTAACA NGG 2 0.0353 Tier II
9 NC_000071.6 5 51646827 - Ppargc1a NNNAATGATTGAGTCTAACA NTG 2 0.0351 Tier II
10 NC_000067.6 1 110033361 - Cdh7 NNNGATGATTGAGAGTAACA NGG 2 0.031 Tier II
11 NC_000079.6 13 23361202 - Zfp322a NNNGAGGATTGAGTGTAACA NGG 2 0.0286 Tier II
12 NC_000067.6 1 70706483 + Spag16 NNNGATTATTGAGTGTAACA NGG 2 0.0286 Tier II
13 NC_000068.7 2 11427857 + Gm31990 NNNGATGAGTGAGTCTAACA NGG 1 0.619 Tier III
14 NC_000070.6 4 78345005 + Gm32545 NNNGATGATTGAATCTAACA NAG 2 0.2393 Tier III
15 NC_000086.7 X 105162336 - Gm33605 NNNGATGCTTGAGTCTCACA NGG 2 0.0756 Tier III
16 NC_000079.6 13 23361202 - 4930586N03Rik NNNGAGGATTGAGTGTAACA NGG 2 0.0286 Tier III
Download CSV

Other clones with same target sequence:

(none)

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