Construct: sgRNA BRDN0001147308

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TAAATGTGTACCTAATGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PBK (55872)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75574

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 27828110 - PBK NNNATGTGTACCTAATGAAA NGG 0 1.0 Tier I
2 NC_000002.12 2 229580494 + DNER NNNATGTGTACCTAATAAAA NGG 1 0.9333 Tier II
3 NC_000009.12 9 95895546 - ERCC6L2 NNNATGTGTACCTAATAAAA NGG 1 0.9333 Tier II
4 NC_000013.11 13 101503022 + ITGBL1 NNNATGTGTACCCAATAAAA NGG 2 0.7368 Tier II
5 NC_000011.10 11 92504745 - FAT3 NNNATGTGTACCAAATGAAA NGG 1 0.6923 Tier II
6 NC_000002.12 2 230745115 - CAB39 NNNATGAGTACTTAATGAAA NGG 2 0.4712 Tier II
7 NC_000011.10 11 119257247 - CBL NNNATGTGTACATAGTGAAA NGG 2 0.4643 Tier II
8 NC_000001.11 1 66583587 - SGIP1 NNNAAGTTTACCTAATGAAA NGG 2 0.3125 Tier II
9 NC_000001.11 1 171498419 - PRRC2C NNNATGTGTGCCTTATGAAA NGG 2 0.2963 Tier II
10 NC_000002.12 2 189785494 - PMS1 NNNATGTGTACCAAATGGAA NGG 2 0.2769 Tier II
11 NC_000012.12 12 86607141 - MGAT4C NNNATGTGTTTCTAATGAAA NGG 2 0.2715 Tier II
12 NC_000007.14 7 111292597 + IMMP2L NNNATGTGTCACTAATGAAA NGG 2 0.25 Tier II
13 NC_000005.10 5 19553164 - CDH18 NNNATGTATACCTAATCAAA NGG 2 0.2353 Tier II
14 NC_000006.12 6 17965169 + KIF13A NNNATGTGTCCCAAATGAAA NGG 2 0.2308 Tier II
15 NC_000010.11 10 93779645 + LGI1 NNNATGTGTATCAAATGAAA NGG 2 0.213 Tier II
16 NC_000007.14 7 21939476 + CDCA7L NNNATGAGTACCTAATGAAC NGG 2 0.1989 Tier II
17 NC_000003.12 3 100871245 - ABI3BP NNNATGTGTACCTAGTGAAA NAG 2 0.1685 Tier II
18 NC_000001.11 1 197048611 + F13B NNNGTGTGTACCTAATGAAA NAG 2 0.162 Tier II
19 NC_000023.11 X 112106127 + RTL4 NNNTTGTGTACCTATTGAAA NGG 2 0.1273 Tier II
20 NC_000014.9 14 70998832 + PCNX1 NNNATGTGTACCTAATTAAA NAG 2 0.0648 Tier II
21 NC_000010.11 10 75432837 - LRMDA NNNATGTGTACCTGATGAAA NGA 2 0.0324 Tier II
22 NC_000004.12 4 47271983 - GABRB1 NNNATGTGTAACTAATGAAA NTG 2 0.0292 Tier II
23 NC_000009.12 9 83864423 - KIF27 NNNCTGTGTACCTAATGAAA NGA 2 0.0245 Tier II
24 NC_000001.11 1 26414602 - LIN28A NNNATGTGTACCTAAGGAAA NGA 2 0.024 Tier II
25 NC_000001.11 1 154464509 + IL6R NNNATGTGTACCTAATGAAC NGC 2 0.0051 Tier II
26 NC_000006.12 6 141428028 + LOC105378029 NNNATGTATACCCAATGAAA NGG 2 0.7895 Tier III
27 NC_000010.11 10 64853687 + LOC105378336 NNNAAGTATACCTAATGAAA NGG 2 0.5 Tier III
28 NC_000006.12 6 79456404 + LOC100506851 NNNATGAGTACTTAATGAAA NGG 2 0.4712 Tier III
29 NC_000020.11 20 9988375 + PARAL1 NNNATGTGTTCCTAATGTAA NGG 2 0.4412 Tier III
30 NC_000008.11 8 12652771 - LOC729732 NNNATGTGTACATTATGAAA NGG 2 0.381 Tier III
31 NC_000010.11 10 93779645 + LOC105378437 NNNATGTGTATCAAATGAAA NGG 2 0.213 Tier III
32 NC_000006.12 6 67334981 + LOC105377842 NNNATTTGTACCTAATGAAA NAG 2 0.1728 Tier III
33 NC_000018.10 18 45778779 - LOC105372093 NNNATGTGTACCTATTCAAA NGG 2 0.0471 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 45088332 + Supt3 NNNATATGTACCTAATAAAA NGG 2 0.9333 Tier II
2 NC_000078.6 12 119420970 - Macc1 NNNATCTGTTCCTAATGAAA NGG 2 0.6016 Tier II
3 NC_000080.6 14 17022630 - Rarb NNNATTTGTTCCTAATGAAA NGG 2 0.5882 Tier II
4 NC_000083.6 17 68739294 - L3mbtl4 NNNATGTATACCTTATGAAA NGG 2 0.5333 Tier II
5 NC_000069.6 3 5288348 - Zfhx4 NNNATGAGTACTTAATGAAA NGG 2 0.4712 Tier II
6 NC_000068.7 2 157214815 + Mroh8 NNNCTGTGTACCTAAAGAAA NGG 2 0.3209 Tier II
7 NC_000075.6 9 67329646 - Tln2 NNNCTGTGTAACTAATGAAA NGG 2 0.2647 Tier II
8 NC_000070.6 4 76035879 + Ptprd NNNATGTGTACCTAAACAAA NGG 2 0.2139 Tier II
9 NC_000078.6 12 69678726 + Sos2 NNNATTTGTATCTAATGAAA NGG 2 0.2051 Tier II
10 NC_000086.7 X 159857314 + Sh3kbp1 NNNATGTGTATCTAATGAAC NGG 2 0.0699 Tier II
11 NC_000079.6 13 108053073 + Ndufaf2 NNNATGTGTAGCTAATGAAC NGG 2 0.0568 Tier II
12 NC_000075.6 9 83935126 - Bckdhb NNNTTGTGTACCTAATGAAA NTG 2 0.0248 Tier II
13 NC_000072.6 6 86114768 - Add2 NNNATGTGTCCCTAATGAAA NGA 2 0.0231 Tier II
14 NC_000082.6 16 6913158 + Rbfox1 NNNATGTGTACCGAATGAAA NTG 2 0.0102 Tier II
15 NC_000084.6 18 11921461 - Cables1 NNNATGTGTACCTAATGAAA NCT 2 0.0 Tier II
16 NC_000070.6 4 94094308 - Gm12648 NNNATATGTTCCTAATGAAA NGG 2 0.8824 Tier III
17 NC_000079.6 13 10480134 + Gm26861 NNNATGACTACCTAATGAAA NGG 2 0.5385 Tier III
18 NC_000072.6 6 127162411 + 9330179D12Rik NNNATGTGTAGCTAGTGAAA NGG 2 0.1625 Tier III
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Other clones with same target sequence:

(none)