Construct: sgRNA BRDN0001147308
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TAAATGTGTACCTAATGAAA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- PBK (55872)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000008.11 | 8 | 27828110 | - | PBK | NNNATGTGTACCTAATGAAA | NGG | 0 | 1.0 | Tier I |
2 | NC_000002.12 | 2 | 229580494 | + | DNER | NNNATGTGTACCTAATAAAA | NGG | 1 | 0.9333 | Tier II |
3 | NC_000009.12 | 9 | 95895546 | - | ERCC6L2 | NNNATGTGTACCTAATAAAA | NGG | 1 | 0.9333 | Tier II |
4 | NC_000013.11 | 13 | 101503022 | + | ITGBL1 | NNNATGTGTACCCAATAAAA | NGG | 2 | 0.7368 | Tier II |
5 | NC_000011.10 | 11 | 92504745 | - | FAT3 | NNNATGTGTACCAAATGAAA | NGG | 1 | 0.6923 | Tier II |
6 | NC_000002.12 | 2 | 230745115 | - | CAB39 | NNNATGAGTACTTAATGAAA | NGG | 2 | 0.4712 | Tier II |
7 | NC_000011.10 | 11 | 119257247 | - | CBL | NNNATGTGTACATAGTGAAA | NGG | 2 | 0.4643 | Tier II |
8 | NC_000001.11 | 1 | 66583587 | - | SGIP1 | NNNAAGTTTACCTAATGAAA | NGG | 2 | 0.3125 | Tier II |
9 | NC_000001.11 | 1 | 171498419 | - | PRRC2C | NNNATGTGTGCCTTATGAAA | NGG | 2 | 0.2963 | Tier II |
10 | NC_000002.12 | 2 | 189785494 | - | PMS1 | NNNATGTGTACCAAATGGAA | NGG | 2 | 0.2769 | Tier II |
11 | NC_000012.12 | 12 | 86607141 | - | MGAT4C | NNNATGTGTTTCTAATGAAA | NGG | 2 | 0.2715 | Tier II |
12 | NC_000007.14 | 7 | 111292597 | + | IMMP2L | NNNATGTGTCACTAATGAAA | NGG | 2 | 0.25 | Tier II |
13 | NC_000005.10 | 5 | 19553164 | - | CDH18 | NNNATGTATACCTAATCAAA | NGG | 2 | 0.2353 | Tier II |
14 | NC_000006.12 | 6 | 17965169 | + | KIF13A | NNNATGTGTCCCAAATGAAA | NGG | 2 | 0.2308 | Tier II |
15 | NC_000010.11 | 10 | 93779645 | + | LGI1 | NNNATGTGTATCAAATGAAA | NGG | 2 | 0.213 | Tier II |
16 | NC_000007.14 | 7 | 21939476 | + | CDCA7L | NNNATGAGTACCTAATGAAC | NGG | 2 | 0.1989 | Tier II |
17 | NC_000003.12 | 3 | 100871245 | - | ABI3BP | NNNATGTGTACCTAGTGAAA | NAG | 2 | 0.1685 | Tier II |
18 | NC_000001.11 | 1 | 197048611 | + | F13B | NNNGTGTGTACCTAATGAAA | NAG | 2 | 0.162 | Tier II |
19 | NC_000023.11 | X | 112106127 | + | RTL4 | NNNTTGTGTACCTATTGAAA | NGG | 2 | 0.1273 | Tier II |
20 | NC_000014.9 | 14 | 70998832 | + | PCNX1 | NNNATGTGTACCTAATTAAA | NAG | 2 | 0.0648 | Tier II |
21 | NC_000010.11 | 10 | 75432837 | - | LRMDA | NNNATGTGTACCTGATGAAA | NGA | 2 | 0.0324 | Tier II |
22 | NC_000004.12 | 4 | 47271983 | - | GABRB1 | NNNATGTGTAACTAATGAAA | NTG | 2 | 0.0292 | Tier II |
23 | NC_000009.12 | 9 | 83864423 | - | KIF27 | NNNCTGTGTACCTAATGAAA | NGA | 2 | 0.0245 | Tier II |
24 | NC_000001.11 | 1 | 26414602 | - | LIN28A | NNNATGTGTACCTAAGGAAA | NGA | 2 | 0.024 | Tier II |
25 | NC_000001.11 | 1 | 154464509 | + | IL6R | NNNATGTGTACCTAATGAAC | NGC | 2 | 0.0051 | Tier II |
26 | NC_000006.12 | 6 | 141428028 | + | LOC105378029 | NNNATGTATACCCAATGAAA | NGG | 2 | 0.7895 | Tier III |
27 | NC_000010.11 | 10 | 64853687 | + | LOC105378336 | NNNAAGTATACCTAATGAAA | NGG | 2 | 0.5 | Tier III |
28 | NC_000006.12 | 6 | 79456404 | + | LOC100506851 | NNNATGAGTACTTAATGAAA | NGG | 2 | 0.4712 | Tier III |
29 | NC_000020.11 | 20 | 9988375 | + | PARAL1 | NNNATGTGTTCCTAATGTAA | NGG | 2 | 0.4412 | Tier III |
30 | NC_000008.11 | 8 | 12652771 | - | LOC729732 | NNNATGTGTACATTATGAAA | NGG | 2 | 0.381 | Tier III |
31 | NC_000010.11 | 10 | 93779645 | + | LOC105378437 | NNNATGTGTATCAAATGAAA | NGG | 2 | 0.213 | Tier III |
32 | NC_000006.12 | 6 | 67334981 | + | LOC105377842 | NNNATTTGTACCTAATGAAA | NAG | 2 | 0.1728 | Tier III |
33 | NC_000018.10 | 18 | 45778779 | - | LOC105372093 | NNNATGTGTACCTATTCAAA | NGG | 2 | 0.0471 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000083.6 | 17 | 45088332 | + | Supt3 | NNNATATGTACCTAATAAAA | NGG | 2 | 0.9333 | Tier II |
2 | NC_000078.6 | 12 | 119420970 | - | Macc1 | NNNATCTGTTCCTAATGAAA | NGG | 2 | 0.6016 | Tier II |
3 | NC_000080.6 | 14 | 17022630 | - | Rarb | NNNATTTGTTCCTAATGAAA | NGG | 2 | 0.5882 | Tier II |
4 | NC_000083.6 | 17 | 68739294 | - | L3mbtl4 | NNNATGTATACCTTATGAAA | NGG | 2 | 0.5333 | Tier II |
5 | NC_000069.6 | 3 | 5288348 | - | Zfhx4 | NNNATGAGTACTTAATGAAA | NGG | 2 | 0.4712 | Tier II |
6 | NC_000068.7 | 2 | 157214815 | + | Mroh8 | NNNCTGTGTACCTAAAGAAA | NGG | 2 | 0.3209 | Tier II |
7 | NC_000075.6 | 9 | 67329646 | - | Tln2 | NNNCTGTGTAACTAATGAAA | NGG | 2 | 0.2647 | Tier II |
8 | NC_000070.6 | 4 | 76035879 | + | Ptprd | NNNATGTGTACCTAAACAAA | NGG | 2 | 0.2139 | Tier II |
9 | NC_000078.6 | 12 | 69678726 | + | Sos2 | NNNATTTGTATCTAATGAAA | NGG | 2 | 0.2051 | Tier II |
10 | NC_000086.7 | X | 159857314 | + | Sh3kbp1 | NNNATGTGTATCTAATGAAC | NGG | 2 | 0.0699 | Tier II |
11 | NC_000079.6 | 13 | 108053073 | + | Ndufaf2 | NNNATGTGTAGCTAATGAAC | NGG | 2 | 0.0568 | Tier II |
12 | NC_000075.6 | 9 | 83935126 | - | Bckdhb | NNNTTGTGTACCTAATGAAA | NTG | 2 | 0.0248 | Tier II |
13 | NC_000072.6 | 6 | 86114768 | - | Add2 | NNNATGTGTCCCTAATGAAA | NGA | 2 | 0.0231 | Tier II |
14 | NC_000082.6 | 16 | 6913158 | + | Rbfox1 | NNNATGTGTACCGAATGAAA | NTG | 2 | 0.0102 | Tier II |
15 | NC_000084.6 | 18 | 11921461 | - | Cables1 | NNNATGTGTACCTAATGAAA | NCT | 2 | 0.0 | Tier II |
16 | NC_000070.6 | 4 | 94094308 | - | Gm12648 | NNNATATGTTCCTAATGAAA | NGG | 2 | 0.8824 | Tier III |
17 | NC_000079.6 | 13 | 10480134 | + | Gm26861 | NNNATGACTACCTAATGAAA | NGG | 2 | 0.5385 | Tier III |
18 | NC_000072.6 | 6 | 127162411 | + | 9330179D12Rik | NNNATGTGTAGCTAGTGAAA | NGG | 2 | 0.1625 | Tier III |