Construct: sgRNA BRDN0001147313

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCGACTTTGGACTGGAGTGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DGKI (9162)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76616

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 137689895 - DGKI NNNACTTTGGACTGGAGTGT NGG 0 1.0 Tier I
2 NC_000002.12 2 25653855 + DTNB NNNACCTGGGACTGGAGTGT NGG 2 0.6667 Tier II
3 NC_000008.11 8 91103325 + LRRC69 NNNACTTTGAAATGGAGTGT NGG 2 0.6667 Tier II
4 NC_000012.12 12 107389562 - ABTB3 NNNACTCTGGACAGGAGTGT NGG 2 0.476 Tier II
5 NC_000017.11 17 57265905 + MSI2 NNNCCTTTGGACTGGAGTGT NGG 1 0.3529 Tier II
6 NC_000020.11 20 44558532 - PKIG NNNAGGTTGGACTGGAGTGT NGG 2 0.3429 Tier II
7 NC_000004.12 4 4596525 - LOC124900165 NNNTCTTTGGACTGGAGTCT NGG 2 0.2853 Tier II
8 NC_000007.14 7 147743883 + CNTNAP2 NNNACTGTGGAGTGGAGTGT NGG 2 0.2614 Tier II
9 NC_000005.10 5 131852661 + MEIKIN NNNTCTTTGGACTGGAGGGT NGG 2 0.2121 Tier II
10 NC_000002.12 2 54356471 - CIMIP6 NNNAATTTGGACTGGAGGGT NGG 2 0.1905 Tier II
11 NC_000014.9 14 81221263 - GTF2A1 NNNGCTTTGGACTGGAGTGT NAG 2 0.162 Tier II
12 NC_000006.12 6 162388903 - PRKN NNNACTTTGGACTGGACAGT NGG 2 0.1569 Tier II
13 NC_000005.10 5 34128840 + C1QTNF3 NNNACTTTGGACTGGACTTT NGG 2 0.1569 Tier II
14 NC_000004.12 4 181620371 - TENM3 NNNACTGTGGACTGGAGTGT NTG 2 0.0229 Tier II
15 NC_000014.9 14 32665260 - AKAP6 NNNACTTTCGACTGGTGTGT NGG 2 0.0 Tier II
16 NC_000006.12 6 39224544 + KCNK5 NNNACTTTGGACTGGAGTGT NTA 2 0.0 Tier II
17 NC_000016.10 16 47367002 - ITFG1 NNNACTTTGGACTGGTGTCT NGG 2 0.0 Tier II
18 NC_000003.12 3 147499198 + LOC440982 NNNTCTTTGGACTGGAATGT NGG 2 0.5939 Tier III
19 NC_000011.10 11 62119589 - LOC124902681 NNNACTTGAGACTGGAGTGT NGG 2 0.4714 Tier III
20 NC_000008.11 8 83917736 - LOC124901968 NNNACTTGGGACTGGAGTGA NGG 2 0.4125 Tier III
21 NC_000004.12 4 4596525 - STX18-AS1 NNNTCTTTGGACTGGAGTCT NGG 2 0.2853 Tier III
22 NC_000017.11 17 16976898 - LOC284191 NNNACTTAGGACTGCAGTGT NGG 2 0.2182 Tier III
23 NC_000020.11 20 41940166 + LOC101927182 NNNACTTTGGACTGCAGAGT NGG 2 0.1818 Tier III
24 NC_000014.9 14 81221263 - GTF2A1-AS1 NNNGCTTTGGACTGGAGTGT NAG 2 0.162 Tier III
25 NC_000006.12 6 162388903 - LOC105369171 NNNACTTTGGACTGGACAGT NGG 2 0.1569 Tier III
26 NC_000006.12 6 26836588 + LOC124901290 NNNACTTTGGACTGGACTTT NGG 2 0.1569 Tier III
27 NC_000010.11 10 112317153 - GUCY2GP NNNAATTTGGACTGGACTGT NGG 2 0.1345 Tier III
28 NC_000003.12 3 117655949 + LOC124906270 NNNAGTTTGGACTGGAGTGG NGG 2 0.1059 Tier III
29 NC_000001.11 1 208840320 - LOC107985255 NNNCCTTTGGACTGGAGTGT NAG 2 0.0915 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 37149550 - Dgki NNNACTCTGGACTGGAGCGT NGG 2 0.2946 Tier I
2 NC_000068.7 2 91944762 - Dgkz NNNACTGTGGACTGGAGTGT NAG 2 0.1525 Tier I
3 NC_000074.6 8 119130815 - Cdh13 NNNACATAGGACTGGAGTGT NGG 2 0.6933 Tier II
4 NC_000086.7 X 162875383 - Syap1 NNNACTTAGGACTGGAGAGT NGG 2 0.5333 Tier II
5 NC_000068.7 2 90719355 + Nup160 NNNACTTAGGACTGGAGTTT NGG 2 0.5333 Tier II
6 NC_000071.6 5 75008963 - Chic2 NNNACGTTGGACTGGAGAGT NGG 2 0.381 Tier II
7 NC_000073.6 7 90237424 - Ccdc83 NNNACGTTGGACTGGAGAGT NGG 2 0.381 Tier II
8 NC_000071.6 5 108179204 + Ccdc18 NNNACTTTGGACTGGAGAGA NGG 2 0.375 Tier II
9 NC_000077.6 11 96099172 + Calcoco2 NNNACTTTGGACTGGAGAGA NGG 2 0.375 Tier II
10 NC_000084.6 18 24798340 + Fhod3 NNNACTTTGGACTGGAGAGA NGG 2 0.375 Tier II
11 NC_000085.6 19 28504782 - Glis3 NNNACTTTGGGCTGGAGTGA NGG 2 0.3656 Tier II
12 NC_000086.7 X 152002763 + Hsd17b10 NNNGCTTTGGACTGGAGTCT NGG 2 0.2802 Tier II
13 NC_000072.6 6 113706380 + Tatdn2 NNNCCTTGGGACTGGAGTGT NGG 2 0.2588 Tier II
14 NC_000069.6 3 83279870 + Dchs2 NNNACTTTCCACTGGAGTGT NGG 2 0.2154 Tier II
15 NC_000086.7 X 9243088 + Lancl3 NNNACTTTGGACTGGAGCCT NGG 2 0.1921 Tier II
16 NC_000069.6 3 18204665 - Cyp7b1 NNNACTTTGGACTGGATTGT NAG 2 0.0648 Tier II
17 NC_000076.6 10 74996721 + Gnaz NNNACTTTGGGCTGGAGTGC NGG 2 0.0591 Tier II
18 NC_000076.6 10 74996721 + Rsph14 NNNACTTTGGGCTGGAGTGC NGG 2 0.0591 Tier II
19 NC_000069.6 3 102507282 + Ngf NNNACTTGGGACTGGAGTGT NGA 2 0.0509 Tier II
20 NC_000068.7 2 90719355 + Gm39872 NNNACTTAGGACTGGAGTTT NGG 2 0.5333 Tier III
21 NC_000067.6 1 51325714 + 9330175M20Rik NNNACTTTGGGCTGGAGAGT NGG 2 0.4333 Tier III
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Other clones with same target sequence:

(none)