Construct: sgRNA BRDN0001147317

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGATGAATATGGTTATGAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TSSK4 (283629)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77045

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000014.9 14 24206000 + TSSK4 NNNTGAATATGGTTATGAGG NGG 0 1.0 Tier I
2 NC_000006.12 6 55804774 + BMP5 NNNTGAATATGGTTATGATG NGG 1 0.6667 Tier II
3 NC_000006.12 6 57103860 - ZNF451 NNNTGAATATGGTTATGATG NGG 1 0.6667 Tier II
4 NC_000001.11 1 151538219 + CGN NNNTGGATATGGTTATAAGG NGG 2 0.6667 Tier II
5 NC_000015.10 15 50208238 - SLC27A2 NNNTGAATATGGTTAAGAAG NGG 2 0.6494 Tier II
6 NC_000017.11 17 35815715 - TAF15 NNNTGAAAAGGGTTATGAGG NGG 2 0.4 Tier II
7 NC_000010.11 10 87786342 - ATAD1 NNNTGAATATGTTTATGAAG NGG 2 0.2747 Tier II
8 NC_000002.12 2 160112530 + ITGB6 NNNTGATTATGGTAATGAGG NGG 2 0.2708 Tier II
9 NC_000005.10 5 128467093 - FBN2 NNNTGAATTTGGTTATGAGG NAG 2 0.1556 Tier II
10 NC_000001.11 1 28491487 - PHACTR4 NNNTGATTATGGTTATCAGG NGG 2 0.1029 Tier II
11 NC_000003.12 3 169923086 - SAMD7 NNNTGAATATGGTTTTGGGG NGG 2 0.08 Tier II
12 NC_000020.11 20 14429410 - MACROD2 NNNTGAATATGGTTATCAGG NTG 2 0.0092 Tier II
13 NC_000005.10 5 125127812 - LOC101927421 NNNTGTATATGGTTATGGGG NGG 2 0.2857 Tier III
14 NC_000010.11 10 87786342 - LOC124902476 NNNTGAATATGTTTATGAAG NGG 2 0.2747 Tier III
15 NC_000017.11 17 57081957 - AKAP1-DT NNNTGAATATGGTTATGAGA NAG 2 0.2431 Tier III
16 NC_000020.11 20 35753489 + RPF2P1 NNNTGAATCTGGTTCTGAGG NGG 2 0.1558 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 55650425 + Tssk4 NNNGGAGTATGGTTATGAGG NGG 2 0.4412 Tier I
2 NC_000080.6 14 23690647 - Kcnma1 NNNTGTATATGGTAATGAGG NGG 2 0.4422 Tier II
3 NC_000077.6 11 47644936 - Sgcd NNNTGAATATGGTTATGAGC NGG 1 0.4286 Tier II
4 NC_000076.6 10 92859555 - Cfap54 NNNTGAATATGCCTATGAGG NGG 2 0.418 Tier II
5 NC_000067.6 1 140524558 - Kcnt2 NNNTGAATATATTTATGAGG NGG 2 0.3846 Tier II
6 NC_000075.6 9 34928958 + Kirrel3 NNNTGAATATGATTATGGGG NGG 2 0.3733 Tier II
7 NC_000082.6 16 88073607 + Grik1 NNNTGAATTTGGTTATGTGG NGG 2 0.3 Tier II
8 NC_000068.7 2 60611809 + Itgb6 NNNTGATTATGGTAATGAGG NGG 2 0.2708 Tier II
9 NC_000070.6 4 59544785 - Ptbp3 NNNTGAATATGTTTATGAGT NGG 2 0.2692 Tier II
10 NC_000075.6 9 48300997 + Nxpe4 NNNTGAACATGGTTATGAGG NAG 2 0.2593 Tier II
11 NC_000073.6 7 62766259 - A26c2 NNNTGAATATGGTTATGTCG NGG 2 0.2241 Tier II
12 NC_000070.6 4 20578327 - Nkain3 NNNTGAAAATGGGTATGAGG NGG 2 0.2087 Tier II
13 NC_000074.6 8 108319800 - Zfhx3 NNNTAAATATGGTTATCAGG NGG 2 0.2039 Tier II
14 NC_000068.7 2 105353921 + Them7 NNNTGAATATGGTTATGAAG NAG 2 0.1852 Tier II
15 NC_000068.7 2 110871835 + Ano3 NNNTGAATATGGGTATGATG NGG 2 0.1739 Tier II
16 NC_000073.6 7 136935695 + Mgmt NNNTGAATATGGTTATCAGG NAG 2 0.061 Tier II
17 NC_000069.6 3 26655018 - Spata16 NNNTGAATATGGTTATGAGA NTG 2 0.0365 Tier II
18 NC_000081.6 15 18090624 - 4921515E04Rik NNNAGAATATGGCTATGAGG NGG 2 0.3759 Tier III
19 NC_000075.6 9 34928958 + Kirrel3os NNNTGAATATGATTATGGGG NGG 2 0.3733 Tier III
20 NC_000068.7 2 138425674 - LOC102636024 NNNTGATTATGGTTATGGGG NGG 2 0.175 Tier III
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Other clones with same target sequence:

(none)