Construct: sgRNA BRDN0001147318
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGAGGGCGACTACAACGTGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CSNK1E (1454)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77977
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000022.11 |
22 |
38302962 |
- |
CSNK1E |
NNNGGGCGACTACAACGTGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000022.11 |
22 |
38302962 |
- |
TPTEP2-CSNK1E |
NNNGGGCGACTACAACGTGA |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000017.11 |
17 |
82255530 |
- |
CSNK1D |
NNNGGGGGACTACAACGTCA |
NGG |
2 |
0.211 |
Tier I |
| 4 |
NC_000019.10 |
19 |
10296456 |
+ |
ICAM5 |
NNNGGGCGAGTACAACGTGC |
NGG |
2 |
0.0884 |
Tier I |
| 5 |
NC_000005.10 |
5 |
90679792 |
- |
ADGRV1 |
NNNGGGTGACTACAACATGA |
NGG |
2 |
0.7583 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
79429813 |
- |
Csnk1e |
NNNGGGAGACTATAACGTGA |
NGG |
2 |
0.525 |
Tier I |
| 2 |
NC_000082.6 |
16 |
67071969 |
+ |
Cadm2 |
NNNGGGTGACTACAACATGA |
NGG |
2 |
0.7583 |
Tier II |
| 3 |
NC_000074.6 |
8 |
108438178 |
- |
Zfhx3 |
NNNGGGCGACTAGAAGGTGA |
NGG |
2 |
0.021 |
Tier II |
| 4 |
NC_000080.6 |
14 |
62641956 |
- |
Gm52153 |
NNNGGGACACTACAACGTGA |
NGG |
2 |
0.4615 |
Tier III |
Other clones with same target sequence:
(none)
