Construct: sgRNA BRDN0001147320
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGTCGAACTGTCACTGTCCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BRD4 (23476)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77344
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
15257032 |
+ |
BRD4 |
NNNCGAACTGTCACTGTCCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
36146490 |
- |
CAPNS1 |
NNNTGAACTGTCACTGTCTG |
NGG |
2 |
0.3692 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
72442085 |
+ |
Lrrc9 |
NNNCAAACTGTCACTGTCCT |
NGG |
2 |
0.6067 |
Tier II |
| 2 |
NC_000083.6 |
17 |
89167352 |
+ |
Fshr |
NNNAGAACTGTCACTGTCCT |
NGG |
2 |
0.5895 |
Tier II |
| 3 |
NC_000068.7 |
2 |
139702499 |
- |
Ism1 |
NNNCGAACTGTCACTGTCTG |
NGG |
1 |
0.4615 |
Tier II |
| 4 |
NC_000081.6 |
15 |
102333924 |
- |
Myg1 |
NNNCTAACTGTCACTGTCTG |
NGG |
2 |
0.1385 |
Tier II |
| 5 |
NC_000082.6 |
16 |
67190670 |
- |
Cadm2 |
NNNGGAACTGTCACTGTCGG |
NGG |
2 |
0.0625 |
Tier II |
Other clones with same target sequence:
(none)
