Construct: sgRNA BRDN0001147324
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGTTCTCGGGGGCGTCGCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK35 (140901)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77356
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000020.11 |
20 |
2103178 |
- |
STK35 |
NNNTCTCGGGGGCGTCGCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NT_187388.1 |
22 |
173217 |
+ |
LOC124905331 |
NNNTCTCGGCGGCGTCGCGG |
NGG |
2 |
0.15 |
Tier I |
| 3 |
NC_000012.12 |
12 |
131113840 |
- |
ADGRD1 |
NNNTCTCTGGGGCGTCACAG |
NGG |
2 |
0.5833 |
Tier II |
| 4 |
NC_000015.10 |
15 |
78781152 |
- |
ADAMTS7 |
NNNACTTGGGGGCGTCGCAG |
NGG |
2 |
0.3869 |
Tier II |
| 5 |
NC_000021.9 |
21 |
37769574 |
- |
KCNJ6 |
NNNTCTCGGAGGCGTCTCAG |
NGG |
2 |
0.2333 |
Tier II |
| 6 |
NC_000004.12 |
4 |
1631612 |
- |
FAM53A |
NNNTCTCTGGGGCGTCTCAG |
NGG |
2 |
0.1563 |
Tier II |
| 7 |
NC_000006.12 |
6 |
31901521 |
+ |
C2 |
NNNTCTCGGGGGCGGGGCAG |
NGG |
2 |
0.0077 |
Tier II |
| 8 |
NC_000006.12 |
6 |
31901521 |
+ |
ZBTB12 |
NNNTCTCGGGGGCGGGGCAG |
NGG |
2 |
0.0077 |
Tier II |
| 9 |
NC_000006.12 |
6 |
17707109 |
+ |
NUP153-AS1 |
NNNTCTCCGGGGCGCCGCAG |
NGG |
2 |
0.1678 |
Tier III |
| 10 |
NC_000021.9 |
21 |
8211835 |
+ |
RNA45SN2 |
NNNTCTCGGCGGCGTCGCGG |
NGG |
2 |
0.15 |
Tier III |
| 11 |
NC_000021.9 |
21 |
8394870 |
+ |
RNA45SN3 |
NNNTCTCGGCGGCGTCGCGG |
NGG |
2 |
0.15 |
Tier III |
| 12 |
NC_000021.9 |
21 |
8439083 |
+ |
RNA45SN1 |
NNNTCTCGGCGGCGTCGCGG |
NGG |
2 |
0.15 |
Tier III |
| 13 |
NT_167214.1 |
|
111285 |
+ |
LOC100507412 |
NNNTCTCGGCGGCGTCGCGG |
NGG |
2 |
0.15 |
Tier III |
| 14 |
NT_167214.1 |
|
111285 |
+ |
RNA45SN5 |
NNNTCTCGGCGGCGTCGCGG |
NGG |
2 |
0.15 |
Tier III |
| 15 |
NT_187388.1 |
22 |
128138 |
+ |
RNA45SN4 |
NNNTCTCGGCGGCGTCGCGG |
NGG |
2 |
0.15 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
129801817 |
- |
Stk35 |
NNNTCTCGGGAGCGTCGCAG |
NGG |
1 |
1.0 |
Tier I |
| 2 |
NC_000072.6 |
6 |
83587627 |
- |
Gm21284 |
NNNTCTCGGGGGAGACGCAG |
NGG |
2 |
0.2227 |
Tier III |
| 3 |
NC_000072.6 |
6 |
85989247 |
+ |
Gm33319 |
NNNTCTCGGGGGAGACGCAG |
NGG |
2 |
0.2227 |
Tier III |
Other clones with same target sequence:
(none)
