Construct: sgRNA BRDN0001147327
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATACACATCCCATATGGTGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIK3C3 (5289)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76967
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000018.10 |
18 |
41962557 |
- |
PIK3C3 |
NNNCACATCCCATATGGTGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
4752194 |
- |
ITPR1 |
NNNCAAATACCATATGGTGA |
NGG |
2 |
0.7959 |
Tier II |
| 3 |
NC_000006.12 |
6 |
136232973 |
+ |
MTFR2 |
NNNCACATTCCATATGGTTA |
NGG |
2 |
0.5833 |
Tier II |
| 4 |
NC_000007.14 |
7 |
147913986 |
- |
CNTNAP2 |
NNNTACATGCCATATGGTGA |
NGG |
2 |
0.4952 |
Tier II |
| 5 |
NC_000005.10 |
5 |
96524540 |
+ |
CAST |
NNNCACATCCCATATGGGGA |
NGG |
1 |
0.3333 |
Tier II |
| 6 |
NC_000012.12 |
12 |
112740838 |
- |
RPH3A |
NNNGACATCCCATATGGAGA |
NGG |
2 |
0.3333 |
Tier II |
| 7 |
NC_000009.12 |
9 |
14125511 |
- |
NFIB |
NNNCTCAACCCATATGGTGA |
NGG |
2 |
0.2909 |
Tier II |
| 8 |
NC_000017.11 |
17 |
19280823 |
+ |
EPN2 |
NNNCAGATCCCATATGTTGA |
NGG |
2 |
0.125 |
Tier II |
| 9 |
NC_000001.11 |
1 |
22880336 |
+ |
EPHB2 |
NNNCACATCCCATATGGTCA |
NAG |
2 |
0.1162 |
Tier II |
| 10 |
NC_000002.12 |
2 |
68527054 |
+ |
APLF |
NNNCACTTCCCAGATGGTGA |
NGG |
2 |
0.1141 |
Tier II |
| 11 |
NC_000009.12 |
9 |
28150236 |
- |
LINGO2 |
NNNCACTTCCCAGATGGTGA |
NGG |
2 |
0.1141 |
Tier II |
| 12 |
NC_000009.12 |
9 |
92106127 |
- |
SPTLC1 |
NNNCACTTCCCAGATGGTGA |
NGG |
2 |
0.1141 |
Tier II |
| 13 |
NC_000010.11 |
10 |
31361559 |
+ |
ZEB1 |
NNNCACTTCCCAGATGGTGA |
NGG |
2 |
0.1141 |
Tier II |
| 14 |
NC_000008.11 |
8 |
130801110 |
- |
ADCY8 |
NNNCACATTCCATATGGTGA |
NGA |
2 |
0.0608 |
Tier II |
| 15 |
NC_000001.11 |
1 |
240545627 |
- |
GREM2 |
NNNCAGATCCCATAGGGTGA |
NGG |
2 |
0.025 |
Tier II |
| 16 |
NC_000007.14 |
7 |
105747697 |
- |
ATXN7L1 |
NNNCAGATCCCATAGGGTGA |
NGG |
2 |
0.025 |
Tier II |
| 17 |
NC_000011.10 |
11 |
64686460 |
- |
NRXN2 |
NNNCCCATCCCATATGGTGA |
NGC |
2 |
0.0111 |
Tier II |
| 18 |
NC_000023.11 |
X |
54071686 |
- |
FAM120C |
NNNCACATCCCATATGGTGA |
NAA |
2 |
0.0 |
Tier II |
| 19 |
NC_000003.12 |
3 |
172281875 |
- |
FNDC3B |
NNNCACCTCCCATATTGTGA |
NGG |
2 |
0.0 |
Tier II |
| 20 |
NC_000014.9 |
14 |
22715351 |
+ |
OXA1L-DT |
NNNCACTTCCCATATGGTGG |
NGG |
2 |
0.3346 |
Tier III |
| 21 |
NC_000005.10 |
5 |
96524540 |
+ |
LOC101929710 |
NNNCACATCCCATATGGGGA |
NGG |
1 |
0.3333 |
Tier III |
| 22 |
NC_000005.10 |
5 |
57457188 |
- |
RMEL3 |
NNNCACATCCCTTATGGTAA |
NGG |
2 |
0.2381 |
Tier III |
| 23 |
NC_000006.12 |
6 |
131361495 |
- |
LOC105378005 |
NNNCTCATCCCATAAGGTGA |
NGG |
2 |
0.2105 |
Tier III |
| 24 |
NC_000016.10 |
16 |
33752289 |
+ |
LOC102724207 |
NNNCACATCCCAGGTGGTGA |
NGG |
2 |
0.1217 |
Tier III |
| 25 |
NT_187383.1 |
16 |
1598554 |
+ |
LOC102723869 |
NNNCACATCCCAGGTGGTGA |
NGG |
2 |
0.1217 |
Tier III |
| 26 |
NC_000009.12 |
9 |
82430929 |
+ |
NPAP1P8 |
NNNCACTTCCCAGATGGTGA |
NGG |
2 |
0.1141 |
Tier III |
| 27 |
NC_000016.10 |
16 |
60357818 |
+ |
NPAP1L |
NNNCACTTCCCAGATGGTGA |
NGG |
2 |
0.1141 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
30277040 |
- |
Pik3c3 |
NNNCACATCCCATATAGTCA |
NGG |
2 |
0.4483 |
Tier I |
| 2 |
NC_000080.6 |
14 |
121364909 |
+ |
Stk24 |
NNNCAAATCCCACATGGTGA |
NGG |
2 |
0.7331 |
Tier II |
| 3 |
NC_000070.6 |
4 |
101099985 |
- |
Raver2 |
NNNAACATCACATATGGTGA |
NGG |
2 |
0.7298 |
Tier II |
| 4 |
NC_000076.6 |
10 |
9792377 |
- |
Stxbp5 |
NNNCACAGCCCACATGGTGA |
NGG |
2 |
0.5789 |
Tier II |
| 5 |
NC_000079.6 |
13 |
102943411 |
- |
Mast4 |
NNNCACATACCATGTGGTGA |
NGG |
2 |
0.4 |
Tier II |
| 6 |
NC_000084.6 |
18 |
56842625 |
+ |
Marchf3 |
NNNCAGAACCCATATGGTGA |
NGG |
2 |
0.4 |
Tier II |
| 7 |
NC_000068.7 |
2 |
14746380 |
+ |
Cacnb2 |
NNNCAGATCCCACATGGTGA |
NGG |
2 |
0.3947 |
Tier II |
| 8 |
NC_000071.6 |
5 |
137517613 |
+ |
Gigyf1 |
NNNCTCTTCCCATATGGTGA |
NGG |
2 |
0.1591 |
Tier II |
| 9 |
NC_000084.6 |
18 |
78532674 |
+ |
Slc14a2 |
NNNTACATCCCATATGGTGA |
NTG |
2 |
0.0312 |
Tier II |
| 10 |
NC_000071.6 |
5 |
62678896 |
+ |
Arap2 |
NNNCAGATCCCATATGGTGA |
NGC |
2 |
0.0111 |
Tier II |
| 11 |
NC_000080.6 |
14 |
14932206 |
- |
Nek10 |
NNNCACATCCCATATGGTGA |
NAC |
2 |
0.0 |
Tier II |
| 12 |
NC_000086.7 |
X |
151470935 |
+ |
Fam120c |
NNNCACATCCCATATGGTGA |
NAA |
2 |
0.0 |
Tier II |
| 13 |
NC_000079.6 |
13 |
73707264 |
+ |
Slc6a19os |
NNNCACATCCCACATGGTGA |
NGG |
1 |
0.7895 |
Tier III |
| 14 |
NC_000072.6 |
6 |
31267778 |
- |
2210408F21Rik |
NNNCACAGCCAATATGGTGA |
NGG |
2 |
0.55 |
Tier III |
| 15 |
NC_000079.6 |
13 |
102943411 |
- |
Gm30106 |
NNNCACATACCATGTGGTGA |
NGG |
2 |
0.4 |
Tier III |
| 16 |
NC_000068.7 |
2 |
14746380 |
+ |
Gm13269 |
NNNCAGATCCCACATGGTGA |
NGG |
2 |
0.3947 |
Tier III |
| 17 |
NC_000067.6 |
1 |
132552729 |
- |
Gm51646 |
NNNCACAACCCATGTGGTGA |
NGG |
2 |
0.3733 |
Tier III |
| 18 |
NC_000074.6 |
8 |
107805353 |
+ |
Gm39242 |
NNNCACATCCTATATGGTAA |
NGG |
2 |
0.2198 |
Tier III |
| 19 |
NC_000074.6 |
8 |
107805353 |
+ |
Gm42040 |
NNNCACATCCTATATGGTAA |
NGG |
2 |
0.2198 |
Tier III |
Other clones with same target sequence:
(none)
