Construct: sgRNA BRDN0001147329

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTTCTCACCAAAGATGCGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CIB1 (10519)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76950

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 90231366 + CIB1 NNNCTCACCAAAGATGCGGA NGG 0 1.0 Tier I
2 NC_000008.11 8 12190005 + FAM86B1 NNNCTCAGCAAAGATGCAGA NGG 2 0.4451 Tier II
3 NC_000008.11 8 12432279 + FAM86B2 NNNCTCAGCAAAGATGCAGA NGG 2 0.4451 Tier II
4 NC_000012.12 12 19454765 + AEBP2 NNNCTCACCAAAGATACGGC NGG 2 0.2273 Tier II
5 NC_000017.11 17 74818696 - TMEM104 NNNCTCACCAGAGATGCGGC NGG 2 0.1477 Tier II
6 NC_000017.11 17 38769276 + PIP4K2B NNNCTCAGCAAAGATGGGGA NGG 2 0.0378 Tier II
7 NC_000005.10 5 171310634 - TLX3 NNNCTCACGAAAGATGGGGA NGG 2 0.0364 Tier II
8 NC_000004.12 4 16734668 + LDB2 NNNCTCACCAAAGATGAGGA NGA 2 0.0324 Tier II
9 NC_000010.11 10 11512026 + USP6NL NNNCTCACCAAAGATGCCGA NGC 2 0.0106 Tier II
10 NC_000018.10 18 48603766 - CTIF NNNCTCACCAAAGATGAGGA NGC 2 0.0104 Tier II
11 NC_000013.11 13 39252912 - LOC107984579 NNNCTAACTAAAGATGCGGA NGG 2 0.8125 Tier III
12 NC_000004.12 4 9157363 - FAM86KP NNNCTCAGCAAAGATGCAGA NGG 2 0.4451 Tier III
13 NC_000008.11 8 8232678 - FAM86B3P NNNCTCAGCAAAGATGCAGA NGG 2 0.4451 Tier III
14 NC_000008.11 8 12190005 + FAM85A NNNCTCAGCAAAGATGCAGA NGG 2 0.4451 Tier III
15 NC_000011.10 11 71791623 - FAM86C1P NNNCTCAGCAAAGATGCAGA NGG 2 0.4451 Tier III
16 NC_000012.12 12 8238866 + FAM86FP NNNCTCAGCAAAGATGCAGA NGG 2 0.4451 Tier III
17 NC_000012.12 12 8238866 + LINC02449 NNNCTCAGCAAAGATGCAGA NGG 2 0.4451 Tier III
18 NC_000010.11 10 11512026 + LOC105376410 NNNCTCACCAAAGATGCCGA NGC 2 0.0106 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 80228407 + Cib1 NNNCTTACCAAAGATGCGGA NGG 1 0.9286 Tier I
2 NC_000086.7 X 73657080 + Pnck NNNCTCACCAAAGATGCTGT NGG 2 0.4 Tier I
3 NC_000078.6 12 110545956 + Ppp2r5c NNNCACATCAAAGATGCGGA NGG 2 0.4375 Tier II
4 NC_000081.6 15 5208886 + Ttc33 NNNCTCAGCAAAGATGCTGA NGG 2 0.4286 Tier II
5 NC_000068.7 2 66223431 + Ttc21b NNNATCACCAAAGATGTGGA NGG 2 0.393 Tier II
6 NC_000074.6 8 55059038 + Gpm6a NNNCTCATCAAAGATGCGCA NGG 2 0.3922 Tier II
7 NC_000067.6 1 129364030 - Thsd7b NNNCTGACCAAAGATGCGGA NAG 2 0.1296 Tier II
8 NC_000074.6 8 12866433 + Atp11a NNNCACACCAAAGATGGGGA NGG 2 0.0294 Tier II
9 NC_000067.6 1 176598905 - Gm36586 NNNCTCAACAAAGATGCAGA NGG 2 0.45 Tier III
10 NC_000073.6 7 83827013 + Gm39043 NNNCTCACCAAAGATGGGAA NGG 2 0.042 Tier III
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Other clones with same target sequence:

(none)