Construct: sgRNA BRDN0001147329
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTTCTCACCAAAGATGCGGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CIB1 (10519)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76950
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
90231366 |
+ |
CIB1 |
NNNCTCACCAAAGATGCGGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
12190005 |
+ |
FAM86B1 |
NNNCTCAGCAAAGATGCAGA |
NGG |
2 |
0.4451 |
Tier II |
| 3 |
NC_000008.11 |
8 |
12432279 |
+ |
FAM86B2 |
NNNCTCAGCAAAGATGCAGA |
NGG |
2 |
0.4451 |
Tier II |
| 4 |
NC_000012.12 |
12 |
19454765 |
+ |
AEBP2 |
NNNCTCACCAAAGATACGGC |
NGG |
2 |
0.2273 |
Tier II |
| 5 |
NC_000017.11 |
17 |
74818696 |
- |
TMEM104 |
NNNCTCACCAGAGATGCGGC |
NGG |
2 |
0.1477 |
Tier II |
| 6 |
NC_000017.11 |
17 |
38769276 |
+ |
PIP4K2B |
NNNCTCAGCAAAGATGGGGA |
NGG |
2 |
0.0378 |
Tier II |
| 7 |
NC_000005.10 |
5 |
171310634 |
- |
TLX3 |
NNNCTCACGAAAGATGGGGA |
NGG |
2 |
0.0364 |
Tier II |
| 8 |
NC_000004.12 |
4 |
16734668 |
+ |
LDB2 |
NNNCTCACCAAAGATGAGGA |
NGA |
2 |
0.0324 |
Tier II |
| 9 |
NC_000010.11 |
10 |
11512026 |
+ |
USP6NL |
NNNCTCACCAAAGATGCCGA |
NGC |
2 |
0.0106 |
Tier II |
| 10 |
NC_000018.10 |
18 |
48603766 |
- |
CTIF |
NNNCTCACCAAAGATGAGGA |
NGC |
2 |
0.0104 |
Tier II |
| 11 |
NC_000013.11 |
13 |
39252912 |
- |
LOC107984579 |
NNNCTAACTAAAGATGCGGA |
NGG |
2 |
0.8125 |
Tier III |
| 12 |
NC_000004.12 |
4 |
9157363 |
- |
FAM86KP |
NNNCTCAGCAAAGATGCAGA |
NGG |
2 |
0.4451 |
Tier III |
| 13 |
NC_000008.11 |
8 |
8232678 |
- |
FAM86B3P |
NNNCTCAGCAAAGATGCAGA |
NGG |
2 |
0.4451 |
Tier III |
| 14 |
NC_000008.11 |
8 |
12190005 |
+ |
FAM85A |
NNNCTCAGCAAAGATGCAGA |
NGG |
2 |
0.4451 |
Tier III |
| 15 |
NC_000011.10 |
11 |
71791623 |
- |
FAM86C1P |
NNNCTCAGCAAAGATGCAGA |
NGG |
2 |
0.4451 |
Tier III |
| 16 |
NC_000012.12 |
12 |
8238866 |
+ |
FAM86FP |
NNNCTCAGCAAAGATGCAGA |
NGG |
2 |
0.4451 |
Tier III |
| 17 |
NC_000012.12 |
12 |
8238866 |
+ |
LINC02449 |
NNNCTCAGCAAAGATGCAGA |
NGG |
2 |
0.4451 |
Tier III |
| 18 |
NC_000010.11 |
10 |
11512026 |
+ |
LOC105376410 |
NNNCTCACCAAAGATGCCGA |
NGC |
2 |
0.0106 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
80228407 |
+ |
Cib1 |
NNNCTTACCAAAGATGCGGA |
NGG |
1 |
0.9286 |
Tier I |
| 2 |
NC_000086.7 |
X |
73657080 |
+ |
Pnck |
NNNCTCACCAAAGATGCTGT |
NGG |
2 |
0.4 |
Tier I |
| 3 |
NC_000078.6 |
12 |
110545956 |
+ |
Ppp2r5c |
NNNCACATCAAAGATGCGGA |
NGG |
2 |
0.4375 |
Tier II |
| 4 |
NC_000081.6 |
15 |
5208886 |
+ |
Ttc33 |
NNNCTCAGCAAAGATGCTGA |
NGG |
2 |
0.4286 |
Tier II |
| 5 |
NC_000068.7 |
2 |
66223431 |
+ |
Ttc21b |
NNNATCACCAAAGATGTGGA |
NGG |
2 |
0.393 |
Tier II |
| 6 |
NC_000074.6 |
8 |
55059038 |
+ |
Gpm6a |
NNNCTCATCAAAGATGCGCA |
NGG |
2 |
0.3922 |
Tier II |
| 7 |
NC_000067.6 |
1 |
129364030 |
- |
Thsd7b |
NNNCTGACCAAAGATGCGGA |
NAG |
2 |
0.1296 |
Tier II |
| 8 |
NC_000074.6 |
8 |
12866433 |
+ |
Atp11a |
NNNCACACCAAAGATGGGGA |
NGG |
2 |
0.0294 |
Tier II |
| 9 |
NC_000067.6 |
1 |
176598905 |
- |
Gm36586 |
NNNCTCAACAAAGATGCAGA |
NGG |
2 |
0.45 |
Tier III |
| 10 |
NC_000073.6 |
7 |
83827013 |
+ |
Gm39043 |
NNNCTCACCAAAGATGGGAA |
NGG |
2 |
0.042 |
Tier III |
Other clones with same target sequence:
(none)
