Construct: sgRNA BRDN0001147331
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAAAGGCCAAGATTGTCGGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK17A (9263)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77918
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
43595999 |
+ |
STK17A |
NNNAGGCCAAGATTGTCGGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
34425844 |
- |
RPS10 |
NNNAGGCGAAGATTGTCCGA |
NGG |
2 |
0.3061 |
Tier II |
| 3 |
NC_000006.12 |
6 |
34425844 |
- |
RPS10-NUDT3 |
NNNAGGCGAAGATTGTCCGA |
NGG |
2 |
0.3061 |
Tier II |
| 4 |
NC_000011.10 |
11 |
46429199 |
- |
AMBRA1 |
NNNAGGCGAAGATTGTCCGA |
NGG |
2 |
0.3061 |
Tier II |
| 5 |
NC_000016.10 |
16 |
27601968 |
+ |
KATNIP |
NNNAGGCAAAGATTGGCGGA |
NGG |
2 |
0.225 |
Tier II |
| 6 |
NC_000009.12 |
9 |
17490198 |
+ |
CNTLN |
NNNAGGCCAACATTGTAGGA |
NGG |
2 |
0.2 |
Tier II |
| 7 |
NC_000001.11 |
1 |
233305698 |
- |
PCNX2 |
NNNAGGCCAAGATTGGTGGA |
NGG |
2 |
0.1615 |
Tier II |
| 8 |
NC_000006.12 |
6 |
134941633 |
- |
ALDH8A1 |
NNNAGGCCAAGATTGGCGGA |
NCG |
2 |
0.0371 |
Tier II |
| 9 |
NC_000001.11 |
1 |
27620801 |
+ |
FGR |
NNNAGGCCAAGATGGGCGGA |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000011.10 |
11 |
46429199 |
- |
RPS10P19 |
NNNAGGCGAAGATTGTCCGA |
NGG |
2 |
0.3061 |
Tier III |
| 11 |
NC_000002.12 |
2 |
65859988 |
+ |
LINC02934 |
NNNATGCCAAGATTGTTGGA |
NGG |
2 |
0.14 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
33032172 |
- |
Ube2j1 |
NNNAGGCCAGGATTGTCTGA |
NGG |
2 |
0.3704 |
Tier II |
| 2 |
NC_000068.7 |
2 |
120024430 |
+ |
Mapkbp1 |
NNNAGGCCAGGATTGTCGGC |
NGG |
2 |
0.1263 |
Tier II |
| 3 |
NC_000072.6 |
6 |
127858442 |
+ |
Gm46976 |
NNNATGCCAAGACTGTCGGA |
NGG |
2 |
0.2368 |
Tier III |
Other clones with same target sequence:
(none)
