Construct: sgRNA BRDN0001147333
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGCACATAGGGGAAACCGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FGFR4 (2264)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77570
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
177092486 |
- |
FGFR4 |
NNNCACATAGGGGAAACCGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
228058431 |
+ |
WNT3A |
NNNCCCATAGGGGAAACCGT |
NCG |
2 |
0.0536 |
Tier II |
| 3 |
NC_000002.12 |
2 |
10414477 |
- |
HPCAL1 |
NNNCACACAGGGGAAACCGT |
NTG |
2 |
0.039 |
Tier II |
| 4 |
NC_000016.10 |
16 |
8397574 |
- |
LOC105371072 |
NNNGACATAGGGGAAACCAT |
NGG |
2 |
0.3571 |
Tier III |
| 5 |
NC_000002.12 |
2 |
10414477 |
- |
LOC124905972 |
NNNCACACAGGGGAAACCGT |
NTG |
2 |
0.039 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
55160088 |
- |
Fgfr4 |
NNNTACGTAGGGGAAACCGT |
NGG |
2 |
0.5647 |
Tier I |
| 2 |
NC_000079.6 |
13 |
24187099 |
- |
Carmil1 |
NNNCACAGAGGGGAAACCTT |
NGG |
2 |
0.4889 |
Tier II |
| 3 |
NC_000077.6 |
11 |
53135987 |
- |
Fstl4 |
NNNCACATAGGGGAAACCTC |
NGG |
2 |
0.0606 |
Tier II |
| 4 |
NC_000082.6 |
16 |
65549055 |
+ |
Chmp2b |
NNNCACATAGGAGAAACCGT |
NTG |
2 |
0.0364 |
Tier II |
| 5 |
NC_000068.7 |
2 |
158061828 |
- |
Rprd1b |
NNNCACAAAGGGGAAACCGT |
NTG |
2 |
0.0312 |
Tier II |
| 6 |
NC_000079.6 |
13 |
24187099 |
- |
Gm33573 |
NNNCACAGAGGGGAAACCTT |
NGG |
2 |
0.4889 |
Tier III |
| 7 |
NC_000084.6 |
18 |
15342002 |
- |
Gm30230 |
NNNCACATAGTGGAAACCAT |
NGG |
2 |
0.2747 |
Tier III |
Other clones with same target sequence:
(none)
