Construct: sgRNA BRDN0001147334
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGACATCAGCCATCATGTGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RPS6KA2 (6196)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75732
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000006.12 |
6 |
166538745 |
- |
RPS6KA2 |
NNNCATCAGCCATCATGTGA |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000003.12 |
3 |
97743238 |
+ |
EPHA6 |
NNNAATCAGCCATCATGTTA |
NGG |
2 |
0.5614 |
Tier II |
3 |
NC_000008.11 |
8 |
72827149 |
- |
KCNB2 |
NNNCCTCAGCCATCATATGA |
NGG |
2 |
0.4667 |
Tier II |
4 |
NC_000017.11 |
17 |
42798017 |
+ |
COA3 |
NNNCATCATCCACCATGTGA |
NGG |
2 |
0.4211 |
Tier II |
5 |
NC_000009.12 |
9 |
36112215 |
- |
RECK |
NNNCATGAGCAATCATGTGA |
NGG |
2 |
0.3529 |
Tier II |
6 |
NC_000002.12 |
2 |
38867481 |
- |
DHX57 |
NNNAATCAGCCATCATCTGA |
NGG |
2 |
0.1981 |
Tier II |
7 |
NC_000016.10 |
16 |
68380704 |
- |
SMPD3 |
NNNCATCAGCCTTCATGTCA |
NGG |
2 |
0.1494 |
Tier II |
8 |
NC_000017.11 |
17 |
50172104 |
- |
SGCA |
NNNCCTCAGCCATCCTGTGA |
NGG |
2 |
0.1364 |
Tier II |
9 |
NC_000003.12 |
3 |
170047996 |
+ |
GPR160 |
NNNCATCAGCCATCATGTCC |
NGG |
2 |
0.1019 |
Tier II |
10 |
NC_000006.12 |
6 |
1772605 |
+ |
GMDS |
NNNCATCTGCCATCATGTGC |
NGG |
2 |
0.0974 |
Tier II |
11 |
NC_000008.11 |
8 |
1093804 |
+ |
DLGAP2 |
NNNCATCAGCCATCATGGGC |
NGG |
2 |
0.0758 |
Tier II |
12 |
NC_000003.12 |
3 |
50963963 |
+ |
DOCK3 |
NNNCATCAGGCATCATGTGA |
NGA |
2 |
0.027 |
Tier II |
13 |
NC_000014.9 |
14 |
47590701 |
- |
MDGA2 |
NNNCATCAGCCATCATGTCA |
NGC |
2 |
0.01 |
Tier II |
14 |
NC_000016.10 |
16 |
15058377 |
- |
PDXDC1 |
NNNCATCAGCCATCTTGTGA |
NGC |
2 |
0.0044 |
Tier II |
15 |
NC_000003.12 |
3 |
97743238 |
+ |
LOC124906256 |
NNNAATCAGCCATCATGTTA |
NGG |
2 |
0.5614 |
Tier III |
16 |
NC_000008.11 |
8 |
37446932 |
+ |
LINC01605 |
NNNCATGAGCCATCATGTAA |
NGG |
2 |
0.3361 |
Tier III |
17 |
NC_000017.11 |
17 |
50172104 |
- |
H1-9P |
NNNCCTCAGCCATCCTGTGA |
NGG |
2 |
0.1364 |
Tier III |
18 |
NC_000006.12 |
6 |
53415611 |
- |
RN7SKP256 |
NNNCATCAGCCTTCAGGTGA |
NGG |
2 |
0.1154 |
Tier III |
19 |
NC_000003.12 |
3 |
170047996 |
+ |
LOC124909455 |
NNNCATCAGCCATCATGTCC |
NGG |
2 |
0.1019 |
Tier III |
20 |
NC_000006.12 |
6 |
168941934 |
+ |
LOC105378146 |
NNNCATCAGCCCTCCTGTGA |
NGG |
2 |
0.0718 |
Tier III |
21 |
NC_000006.12 |
6 |
168942170 |
+ |
LOC105378146 |
NNNCATCAGCCCTCCTGTGA |
NGG |
2 |
0.0718 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000080.6 |
14 |
69846541 |
+ |
Pebp4 |
NNNAATCAGCCATCAAGTGA |
NGG |
2 |
0.7656 |
Tier II |
2 |
NC_000069.6 |
3 |
68216149 |
+ |
Schip1 |
NNNCATCAGCCATCATGTGG |
NGG |
1 |
0.7647 |
Tier II |
3 |
NC_000069.6 |
3 |
68216149 |
+ |
Iqschfp |
NNNCATCAGCCATCATGTGG |
NGG |
1 |
0.7647 |
Tier II |
4 |
NC_000071.6 |
5 |
117212995 |
- |
Taok3 |
NNNCATCAGACACCATGTGA |
NGG |
2 |
0.6842 |
Tier II |
5 |
NC_000083.6 |
17 |
68336243 |
- |
L3mbtl4 |
NNNCATCAGCCATCAAGTAA |
NGG |
2 |
0.6494 |
Tier II |
6 |
NC_000072.6 |
6 |
47111714 |
- |
Cntnap2 |
NNNCATCAGCAATCATGTGG |
NGG |
2 |
0.5735 |
Tier II |
7 |
NC_000080.6 |
14 |
32036897 |
- |
Galnt15 |
NNNCATCAGCCACCATGAGA |
NGG |
2 |
0.5263 |
Tier II |
8 |
NC_000071.6 |
5 |
127866962 |
+ |
Tmem132d |
NNNCATGAGCCACCATGTGA |
NGG |
2 |
0.3715 |
Tier II |
9 |
NC_000067.6 |
1 |
188052001 |
+ |
Esrrg |
NNNCATGAGCAATCATGTGA |
NGG |
2 |
0.3529 |
Tier II |
10 |
NC_000069.6 |
3 |
119380547 |
- |
Dpyd |
NNNCCTCAGCCATCATGAGA |
NGG |
2 |
0.3333 |
Tier II |
11 |
NC_000072.6 |
6 |
119608716 |
- |
Erc1 |
NNNCATCAGCAATAATGTGA |
NGG |
2 |
0.2625 |
Tier II |
12 |
NC_000086.7 |
X |
134946868 |
- |
Nxf2 |
NNNCATCAGCAATAATGTGA |
NGG |
2 |
0.2625 |
Tier II |
13 |
NC_000078.6 |
12 |
83709109 |
+ |
Psen1 |
NNNCATCACCCATCATGTCA |
NGG |
2 |
0.2414 |
Tier II |
14 |
NC_000068.7 |
2 |
24341991 |
+ |
Il1rn |
NNNCATCAGCTATCATGTGG |
NGG |
2 |
0.2353 |
Tier II |
15 |
NC_000080.6 |
14 |
70122003 |
+ |
Bin3 |
NNNTATCAGCCATCATCTGA |
NGG |
2 |
0.1882 |
Tier II |
16 |
NC_000072.6 |
6 |
125098078 |
- |
Chd4 |
NNNCATCAGCCATCACTTGA |
NGG |
2 |
0.1667 |
Tier II |
17 |
NC_000067.6 |
1 |
132211705 |
- |
Lemd1 |
NNNCATAAGCCATCTTGTGA |
NGG |
2 |
0.15 |
Tier II |
18 |
NC_000079.6 |
13 |
45403020 |
+ |
Mylip |
NNNCATCAGGCATCCTGTGA |
NGG |
2 |
0.1061 |
Tier II |
19 |
NC_000067.6 |
1 |
39553746 |
+ |
Rnf149 |
NNNCATGAGCCATCATGTGA |
NTG |
2 |
0.0183 |
Tier II |
20 |
NC_000070.6 |
4 |
111582880 |
- |
Agbl4 |
NNNCATGAGCCATCATGTGA |
NTG |
2 |
0.0183 |
Tier II |
21 |
NC_000078.6 |
12 |
4864325 |
+ |
Mfsd2b |
NNNCATCAGCCATGAAGTGA |
NGG |
2 |
0.0 |
Tier II |
22 |
NC_000068.7 |
2 |
163383316 |
+ |
Jph2 |
NNNCATCAGCCATGATGTGA |
NGT |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)