Construct: sgRNA BRDN0001147334

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGACATCAGCCATCATGTGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS6KA2 (6196)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75732

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 166538745 - RPS6KA2 NNNCATCAGCCATCATGTGA NGG 0 1.0 Tier I
2 NC_000003.12 3 97743238 + EPHA6 NNNAATCAGCCATCATGTTA NGG 2 0.5614 Tier II
3 NC_000008.11 8 72827149 - KCNB2 NNNCCTCAGCCATCATATGA NGG 2 0.4667 Tier II
4 NC_000017.11 17 42798017 + COA3 NNNCATCATCCACCATGTGA NGG 2 0.4211 Tier II
5 NC_000009.12 9 36112215 - RECK NNNCATGAGCAATCATGTGA NGG 2 0.3529 Tier II
6 NC_000002.12 2 38867481 - DHX57 NNNAATCAGCCATCATCTGA NGG 2 0.1981 Tier II
7 NC_000016.10 16 68380704 - SMPD3 NNNCATCAGCCTTCATGTCA NGG 2 0.1494 Tier II
8 NC_000017.11 17 50172104 - SGCA NNNCCTCAGCCATCCTGTGA NGG 2 0.1364 Tier II
9 NC_000003.12 3 170047996 + GPR160 NNNCATCAGCCATCATGTCC NGG 2 0.1019 Tier II
10 NC_000006.12 6 1772605 + GMDS NNNCATCTGCCATCATGTGC NGG 2 0.0974 Tier II
11 NC_000008.11 8 1093804 + DLGAP2 NNNCATCAGCCATCATGGGC NGG 2 0.0758 Tier II
12 NC_000003.12 3 50963963 + DOCK3 NNNCATCAGGCATCATGTGA NGA 2 0.027 Tier II
13 NC_000014.9 14 47590701 - MDGA2 NNNCATCAGCCATCATGTCA NGC 2 0.01 Tier II
14 NC_000016.10 16 15058377 - PDXDC1 NNNCATCAGCCATCTTGTGA NGC 2 0.0044 Tier II
15 NC_000003.12 3 97743238 + LOC124906256 NNNAATCAGCCATCATGTTA NGG 2 0.5614 Tier III
16 NC_000008.11 8 37446932 + LINC01605 NNNCATGAGCCATCATGTAA NGG 2 0.3361 Tier III
17 NC_000017.11 17 50172104 - H1-9P NNNCCTCAGCCATCCTGTGA NGG 2 0.1364 Tier III
18 NC_000006.12 6 53415611 - RN7SKP256 NNNCATCAGCCTTCAGGTGA NGG 2 0.1154 Tier III
19 NC_000003.12 3 170047996 + LOC124909455 NNNCATCAGCCATCATGTCC NGG 2 0.1019 Tier III
20 NC_000006.12 6 168941934 + LOC105378146 NNNCATCAGCCCTCCTGTGA NGG 2 0.0718 Tier III
21 NC_000006.12 6 168942170 + LOC105378146 NNNCATCAGCCCTCCTGTGA NGG 2 0.0718 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 69846541 + Pebp4 NNNAATCAGCCATCAAGTGA NGG 2 0.7656 Tier II
2 NC_000069.6 3 68216149 + Schip1 NNNCATCAGCCATCATGTGG NGG 1 0.7647 Tier II
3 NC_000069.6 3 68216149 + Iqschfp NNNCATCAGCCATCATGTGG NGG 1 0.7647 Tier II
4 NC_000071.6 5 117212995 - Taok3 NNNCATCAGACACCATGTGA NGG 2 0.6842 Tier II
5 NC_000083.6 17 68336243 - L3mbtl4 NNNCATCAGCCATCAAGTAA NGG 2 0.6494 Tier II
6 NC_000072.6 6 47111714 - Cntnap2 NNNCATCAGCAATCATGTGG NGG 2 0.5735 Tier II
7 NC_000080.6 14 32036897 - Galnt15 NNNCATCAGCCACCATGAGA NGG 2 0.5263 Tier II
8 NC_000071.6 5 127866962 + Tmem132d NNNCATGAGCCACCATGTGA NGG 2 0.3715 Tier II
9 NC_000067.6 1 188052001 + Esrrg NNNCATGAGCAATCATGTGA NGG 2 0.3529 Tier II
10 NC_000069.6 3 119380547 - Dpyd NNNCCTCAGCCATCATGAGA NGG 2 0.3333 Tier II
11 NC_000072.6 6 119608716 - Erc1 NNNCATCAGCAATAATGTGA NGG 2 0.2625 Tier II
12 NC_000086.7 X 134946868 - Nxf2 NNNCATCAGCAATAATGTGA NGG 2 0.2625 Tier II
13 NC_000078.6 12 83709109 + Psen1 NNNCATCACCCATCATGTCA NGG 2 0.2414 Tier II
14 NC_000068.7 2 24341991 + Il1rn NNNCATCAGCTATCATGTGG NGG 2 0.2353 Tier II
15 NC_000080.6 14 70122003 + Bin3 NNNTATCAGCCATCATCTGA NGG 2 0.1882 Tier II
16 NC_000072.6 6 125098078 - Chd4 NNNCATCAGCCATCACTTGA NGG 2 0.1667 Tier II
17 NC_000067.6 1 132211705 - Lemd1 NNNCATAAGCCATCTTGTGA NGG 2 0.15 Tier II
18 NC_000079.6 13 45403020 + Mylip NNNCATCAGGCATCCTGTGA NGG 2 0.1061 Tier II
19 NC_000067.6 1 39553746 + Rnf149 NNNCATGAGCCATCATGTGA NTG 2 0.0183 Tier II
20 NC_000070.6 4 111582880 - Agbl4 NNNCATGAGCCATCATGTGA NTG 2 0.0183 Tier II
21 NC_000078.6 12 4864325 + Mfsd2b NNNCATCAGCCATGAAGTGA NGG 2 0.0 Tier II
22 NC_000068.7 2 163383316 + Jph2 NNNCATCAGCCATGATGTGA NGT 2 0.0 Tier II
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Other clones with same target sequence:

(none)