Construct: sgRNA BRDN0001147337
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGAGCAGTAATCACTCTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRAG1 (157285)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75792
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
8377324 |
+ |
PRAG1 |
NNNAGCAGTAATCACTCTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
80776701 |
- |
BRWD3 |
NNNAGCAGTAATCACTCGAG |
NGG |
2 |
0.1429 |
Tier II |
| 3 |
NC_000020.11 |
20 |
43646630 |
+ |
IFT52 |
NNNAGCAGTACTCACTCTGG |
NGG |
2 |
0.05 |
Tier II |
| 4 |
NC_000003.12 |
3 |
17779041 |
+ |
LOC124909350 |
NNNAGCAGTAATAACTCTTG |
NGG |
2 |
0.1775 |
Tier III |
| 5 |
NC_000017.11 |
17 |
43704642 |
+ |
LINC02594 |
NNNAGCAGTAATTACTGTCG |
NGG |
2 |
0.0412 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
41209876 |
+ |
Fgl1 |
NNNAGCAGTTCTCACTCTCG |
NGG |
2 |
0.3529 |
Tier I |
| 2 |
NC_000078.6 |
12 |
33430660 |
+ |
Twistnb |
NNNAGCAGTTACCACTCTCG |
NGG |
2 |
0.8359 |
Tier II |
| 3 |
NC_000081.6 |
15 |
84849167 |
- |
Phf21b |
NNNAGCAGTAAGCACTCTCT |
NGG |
2 |
0.35 |
Tier II |
| 4 |
NC_000074.6 |
8 |
119577049 |
+ |
Dnaaf1 |
NNNAGCAGGAATCGCTCTCG |
NGG |
2 |
0.2889 |
Tier II |
| 5 |
NC_000068.7 |
2 |
54937414 |
+ |
Galnt13 |
NNNAGCTGTAATCACTCTCC |
NGG |
2 |
0.1875 |
Tier II |
| 6 |
NC_000072.6 |
6 |
38020753 |
- |
Svopl |
NNNAGCAGTAATCACTCTCA |
NTG |
2 |
0.0365 |
Tier II |
| 7 |
NC_000069.6 |
3 |
59193691 |
+ |
Gpr87 |
NNNAGCAGTAATCTTTCTCG |
NGG |
2 |
0.0356 |
Tier II |
| 8 |
NC_000069.6 |
3 |
59193691 |
+ |
Med12l |
NNNAGCAGTAATCTTTCTCG |
NGG |
2 |
0.0356 |
Tier II |
| 9 |
NC_000070.6 |
4 |
85757712 |
- |
Adamtsl1 |
NNNAGCAGTAATCACTATCG |
NTG |
2 |
0.0182 |
Tier II |
| 10 |
NC_000068.7 |
2 |
143744104 |
- |
Pcsk2 |
NNNAGCAGTAATCACTCCCG |
NGC |
2 |
0.0095 |
Tier II |
| 11 |
NC_000069.6 |
3 |
59193691 |
+ |
Gm51434 |
NNNAGCAGTAATCTTTCTCG |
NGG |
2 |
0.0356 |
Tier III |
Other clones with same target sequence:
(none)
