Construct: sgRNA BRDN0001147338
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGAAGCCGATTACCAGCCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- WNK4 (65266)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75656
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
42787315 |
+ |
WNK4 |
NNNAGCCGATTACCAGCCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
35802127 |
- |
PHF20 |
NNNAGCTAATTACCAGCCAG |
NGG |
2 |
0.8125 |
Tier II |
| 3 |
NC_000020.11 |
20 |
61644579 |
+ |
CDH4 |
NNNAGATGATTACCAGCCAG |
NGG |
2 |
0.7545 |
Tier II |
| 4 |
NC_000019.10 |
19 |
48978768 |
- |
GYS1 |
NNNGGCCAATTACCAGCCAG |
NGG |
2 |
0.625 |
Tier II |
| 5 |
NC_000004.12 |
4 |
173267460 |
+ |
GALNT7 |
NNNGGCAGATTACCAGCCAG |
NGG |
2 |
0.4688 |
Tier II |
| 6 |
NC_000009.12 |
9 |
134780204 |
+ |
COL5A1 |
NNNAGCCGAATTCCAGCCAG |
NGG |
2 |
0.2857 |
Tier II |
| 7 |
NC_000011.10 |
11 |
120662310 |
+ |
GRIK4 |
NNNAGCCGCTTTCCAGCCAG |
NGG |
2 |
0.1905 |
Tier II |
| 8 |
NC_000010.11 |
10 |
64870117 |
- |
LOC105378336 |
NNNAGCTGAATACCAGCCAG |
NGG |
2 |
0.6964 |
Tier III |
| 9 |
NC_000015.10 |
15 |
45916593 |
+ |
LOC105370802 |
NNNAGCAGATTGCCAGCCAG |
NGG |
2 |
0.5417 |
Tier III |
| 10 |
NC_000008.11 |
8 |
116829760 |
+ |
LOC112268030 |
NNNAGCATATTACCAGCCAG |
NGG |
2 |
0.4688 |
Tier III |
| 11 |
NC_000004.12 |
4 |
173267460 |
+ |
LOC124900812 |
NNNGGCAGATTACCAGCCAG |
NGG |
2 |
0.4688 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
101268263 |
+ |
Wnk4 |
NNNAGCTGACTACCAGCCAG |
NGG |
2 |
0.4333 |
Tier I |
| 2 |
NC_000080.6 |
14 |
121264101 |
- |
Farp1 |
NNNAGCCAATTGCCAGCCAG |
NGG |
2 |
0.7222 |
Tier II |
| 3 |
NC_000084.6 |
18 |
56399391 |
- |
Gramd3 |
NNNTGCCGATAACCAGCCAG |
NGG |
2 |
0.4773 |
Tier II |
Other clones with same target sequence:
(none)
