Construct: sgRNA BRDN0001147343
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAGCGTGTTGTGAATCACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ULK4 (54986)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76011
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
41800275 |
- |
ULK4 |
NNNGCGTGTTGTGAATCACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
34540311 |
- |
PARD3 |
NNNGTGTGTTGTGAACCACA |
NGG |
2 |
0.4242 |
Tier II |
| 3 |
NC_000001.11 |
1 |
101924968 |
+ |
OLFM3 |
NNNGAGTGTTGTGGATCACA |
NGG |
2 |
0.2667 |
Tier II |
| 4 |
NC_000001.11 |
1 |
157700933 |
+ |
FCRL3 |
NNNGCGTGGTGTGAATCACA |
NAG |
2 |
0.1605 |
Tier II |
| 5 |
NC_000015.10 |
15 |
83580141 |
- |
SH3GL3 |
NNNGCGGGTTGTGACTCACA |
NGG |
2 |
0.1604 |
Tier II |
| 6 |
NC_000010.11 |
10 |
33260713 |
- |
NRP1 |
NNNGCGTGTTGTGAATCACA |
NAT |
2 |
0.0 |
Tier II |
| 7 |
NC_000008.11 |
8 |
66267383 |
- |
LOC102724687 |
NNNGCGTGTTATGAATAACA |
NGG |
2 |
0.4667 |
Tier III |
| 8 |
NC_000007.14 |
7 |
34620945 |
- |
NPSR1-AS1 |
NNNCCCTGTTGTGAATCACA |
NGG |
2 |
0.361 |
Tier III |
| 9 |
NC_000015.10 |
15 |
76982266 |
+ |
LOC105370904 |
NNNGAGTGTTTTGAATCACA |
NGG |
2 |
0.2198 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
92879393 |
- |
Cfap54 |
NNNCCATGTTGTGAATCACA |
NGG |
2 |
0.5294 |
Tier II |
| 2 |
NC_000071.6 |
5 |
124732957 |
+ |
Dnah10 |
NNNGGCTGTTGTGAATCACA |
NGG |
2 |
0.4091 |
Tier II |
| 3 |
NC_000074.6 |
8 |
23863767 |
+ |
Zmat4 |
NNNGCGTGATGTGAATCCCA |
NGG |
2 |
0.1769 |
Tier II |
| 4 |
NC_000084.6 |
18 |
25239645 |
+ |
AW554918 |
NNNTCGTGTTCTGAATCACA |
NGG |
2 |
0.1558 |
Tier II |
| 5 |
NC_000071.6 |
5 |
29204446 |
- |
Rnf32 |
NNNGCCTGTTGTGAATCACA |
NGA |
2 |
0.0473 |
Tier II |
Other clones with same target sequence:
(none)
