Construct: sgRNA BRDN0001147345
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TATCGGGACACTCTTGGTCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP2K5 (5607)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77263
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
67600723 |
+ |
MAP2K5 |
NNNCGGGACACTCTTGGTCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
154392729 |
+ |
GALNT13 |
NNNCGGTACACTTTTGGTCA |
NGG |
2 |
0.4 |
Tier II |
| 3 |
NC_000016.10 |
16 |
12175231 |
+ |
SNX29 |
NNNTGGGTCACTCTTGGTCA |
NGG |
2 |
0.3429 |
Tier II |
| 4 |
NC_000003.12 |
3 |
12035222 |
+ |
SYN2 |
NNNCGGGTCACTCTGGGTCA |
NGG |
2 |
0.0214 |
Tier II |
| 5 |
NC_000016.10 |
16 |
2719466 |
- |
PRSS27 |
NNNCGGGACACTCTCTGTCA |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
63322230 |
- |
Map2k5 |
NNNCGAGACACCCTTGGTCA |
NGG |
2 |
0.9474 |
Tier I |
| 2 |
NC_000078.6 |
12 |
72656425 |
+ |
Dhrs7 |
NNNCAGGACACACTTGGTCA |
NGG |
2 |
0.6933 |
Tier I |
| 3 |
NC_000077.6 |
11 |
116282018 |
- |
Galr2 |
NNNAGGGACACTCTTAGTCA |
NGG |
2 |
0.8421 |
Tier II |
| 4 |
NC_000073.6 |
7 |
34954469 |
+ |
Pepd |
NNNGGGGACTCTCTTGGTCA |
NGG |
2 |
0.4412 |
Tier II |
| 5 |
NC_000071.6 |
5 |
138267315 |
+ |
Gal3st4 |
NNNTGGGACACTCTTGGTCA |
NAG |
2 |
0.2074 |
Tier II |
| 6 |
NC_000073.6 |
7 |
121089094 |
- |
Otoa |
NNNCGGGACACTCTGGGGCA |
NGG |
2 |
0.0167 |
Tier II |
| 7 |
NC_000071.6 |
5 |
45421751 |
+ |
D5Ertd615e |
NNNAGGAACACTCTTGGTCA |
NGG |
2 |
0.8421 |
Tier III |
| 8 |
NC_000068.7 |
2 |
180393200 |
- |
Mir1a-1hg |
NNNGGGGACACTCTTGGCCA |
NGG |
2 |
0.2143 |
Tier III |
| 9 |
NC_000073.6 |
7 |
84228631 |
- |
LOC117997403 |
NNNCGGGCCACTCTTGGCCA |
NGG |
2 |
0.1837 |
Tier III |
Other clones with same target sequence:
(none)
