Construct: sgRNA BRDN0001147349
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAATCCGACATGGTTACTGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LRPPRC (10128)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77774
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
43946191 |
- |
LRPPRC |
NNNTCCGACATGGTTACTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
61598271 |
+ |
ADGRL3 |
NNNACCGACATGGATACTGG |
NGG |
2 |
0.2948 |
Tier II |
| 3 |
NC_000004.12 |
4 |
172227166 |
+ |
GALNTL6 |
NNNTCCCACATGGTTACTGG |
NTG |
2 |
0.0268 |
Tier II |
| 4 |
NC_000006.12 |
6 |
116215653 |
- |
COL10A1 |
NNNTCCGACATGGTTACTGG |
NCA |
2 |
0.0 |
Tier II |
| 5 |
NC_000006.12 |
6 |
116215653 |
- |
NT5DC1 |
NNNTCCGACATGGTTACTGG |
NCA |
2 |
0.0 |
Tier II |
| 6 |
NC_000008.11 |
8 |
88823764 |
+ |
LOC105375630 |
NNNCCCGACATGGTTACTGC |
NGG |
2 |
0.2773 |
Tier III |
| 7 |
NC_000011.10 |
11 |
129318749 |
- |
LOC105369572 |
NNNTCCAACATGGTTTCTGG |
NGG |
2 |
0.0 |
Tier III |
| 8 |
NC_000006.12 |
6 |
109327738 |
- |
CCDC162P |
NNNTCCTACATGGTTCCTGG |
NGG |
2 |
0.0 |
Tier III |
| 9 |
NC_000006.12 |
6 |
109327738 |
- |
RPL7P28 |
NNNTCCTACATGGTTCCTGG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
84751302 |
- |
Lrpprc |
NNNTCAGACATGGTTATTGG |
NGG |
2 |
0.4333 |
Tier I |
| 2 |
NC_000067.6 |
1 |
63267183 |
- |
Zdbf2 |
NNNTCCGACATGCTTACTGA |
NGG |
2 |
0.3947 |
Tier II |
| 3 |
NC_000080.6 |
14 |
22705138 |
- |
Lrmda |
NNNTCCGACATTGTTAATGG |
NGG |
2 |
0.1795 |
Tier II |
| 4 |
NC_000067.6 |
1 |
63267183 |
- |
Gm11608 |
NNNTCCGACATGCTTACTGA |
NGG |
2 |
0.3947 |
Tier III |
| 5 |
NC_000081.6 |
15 |
98412732 |
- |
1700031M16Rik |
NNNTCAGACATGGTTCCTGG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
