Construct: sgRNA BRDN0001147351
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACACTGCTGGCTATAGTCGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK33 (65975)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76985
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
8440730 |
+ |
STK33 |
NNNCTGCTGGCTATAGTCGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
9182939 |
- |
NTN1 |
NNNCTGCTGGCTGCAGTCGT |
NGG |
2 |
0.1863 |
Tier I |
| 3 |
NC_000001.11 |
1 |
89190193 |
+ |
GBP4 |
NNNCTGCTGGCTATAGTGGT |
NGG |
1 |
0.1333 |
Tier I |
| 4 |
NC_000001.11 |
1 |
89149447 |
+ |
GBP7 |
NNNCTGCTGGCTGTAGTGGT |
NGG |
2 |
0.087 |
Tier I |
| 5 |
NC_000021.9 |
21 |
26002397 |
- |
APP |
NNNCTGCTGGCTATAGTTGA |
NGG |
2 |
0.3616 |
Tier II |
| 6 |
NC_000006.12 |
6 |
150165447 |
+ |
PPP1R14C |
NNNCTGCTGGCTAAAGTCCT |
NGG |
2 |
0.2775 |
Tier II |
| 7 |
NC_000005.10 |
5 |
6481536 |
- |
UBE2QL1 |
NNNCTGCTGGGTATAGTGGT |
NGG |
2 |
0.0333 |
Tier II |
| 8 |
NC_000021.9 |
21 |
40102434 |
+ |
DSCAM |
NNNCTGCTGGGTAGAGTCGT |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000007.14 |
7 |
106573616 |
- |
CTB-30L5.1 |
NNNCTGCTGGCTATAATCCT |
NGG |
2 |
0.4483 |
Tier III |
| 10 |
NC_000001.11 |
1 |
89149447 |
+ |
LOC105378842 |
NNNCTGCTGGCTGTAGTGGT |
NGG |
2 |
0.087 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
109325733 |
+ |
Stk33 |
NNNCTGCTGGCTGTAGTCAT |
NGG |
2 |
0.4658 |
Tier I |
| 2 |
NC_000081.6 |
15 |
74762532 |
+ |
Ly6d |
NNNCTGCTGGCTGTAGTCGG |
NGG |
2 |
0.1151 |
Tier I |
| 3 |
NC_000078.6 |
12 |
79479420 |
+ |
Rad51b |
NNNCTGCTGGCCATAGTCTT |
NGG |
2 |
0.6316 |
Tier II |
| 4 |
NC_000077.6 |
11 |
33541496 |
- |
Gm36005 |
NNNCTGGTGGCTATATTCGT |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
