Construct: sgRNA BRDN0001147353
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAAATTGGTGAAAGTGTACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRPS1 (5631)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76202
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
107639316 |
+ |
PRPS1 |
NNNATTGGTGAAAGTGTACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
125150791 |
+ |
PPP6C |
NNNATTGGCAAAAGTGTACG |
NGG |
2 |
0.8615 |
Tier II |
| 3 |
NC_000002.12 |
2 |
233073801 |
- |
INPP5D |
NNNATTGGAGAAAGTGAACG |
NGG |
2 |
0.4952 |
Tier II |
| 4 |
NC_000012.12 |
12 |
40925256 |
- |
CNTN1 |
NNNATTGGTGAAAGTATATG |
NGG |
2 |
0.4615 |
Tier II |
| 5 |
NC_000002.12 |
2 |
10447417 |
- |
ODC1 |
NNNATTGGTGAAAGTGTAGA |
NGG |
2 |
0.1172 |
Tier II |
| 6 |
NC_000017.11 |
17 |
20255142 |
+ |
SPECC1 |
NNNAGTGGTGAAAGTTTACG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000009.12 |
9 |
125150791 |
+ |
PRPS1P2 |
NNNATTGGCAAAAGTGTACG |
NGG |
2 |
0.8615 |
Tier III |
| 8 |
NC_000014.9 |
14 |
41019911 |
- |
LINC02315 |
NNNATTGGTCAAAGTGTACA |
NGG |
2 |
0.375 |
Tier III |
| 9 |
NC_000004.12 |
4 |
15327123 |
- |
C1QTNF7-AS1 |
NNNATTGGGGTAAGTGTACG |
NGG |
2 |
0.1905 |
Tier III |
| 10 |
NC_000008.11 |
8 |
124852840 |
+ |
LOC105375743 |
NNNATTGGTGAAAGTGTATG |
NTG |
2 |
0.018 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
34985031 |
+ |
Prps1l1 |
NNNATTGGTGAGAGTGTGCG |
NGG |
2 |
0.2889 |
Tier I |
| 2 |
NC_000076.6 |
10 |
93559687 |
- |
Ccdc38 |
NNNATTGGTGAGAGTGTAGG |
NGG |
2 |
0.0903 |
Tier II |
| 3 |
NC_000083.6 |
17 |
31520132 |
+ |
Ndufv3 |
NNNATTGGTGAACGTGGACG |
NGG |
2 |
0.0248 |
Tier II |
| 4 |
NC_000067.6 |
1 |
95624550 |
- |
St8sia4 |
NNNATTGGTGAAAGGTTACG |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000078.6 |
12 |
34985031 |
+ |
Gm33864 |
NNNATTGGTGAGAGTGTGCG |
NGG |
2 |
0.2889 |
Tier III |
Other clones with same target sequence:
(none)
