Construct: sgRNA BRDN0001147355

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCTACTGCCAGAGAACCCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPK1 (5594)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77787

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000022.11 22 21807762 - MAPK1 NNNACTGCCAGAGAACCCTG NGG 0 1.0 Tier I
2 NC_000003.12 3 142508048 + ATR NNNACTGCCAGAGTATCCTG NGG 2 0.1641 Tier I
3 NC_000006.12 6 168305376 + DACT2 NNNACTGTCAGAAAACCCTG NGG 2 0.8077 Tier II
4 NC_000022.11 22 39375304 - SYNGR1 NNNACTGCCAGGGAACCCTG NGG 1 0.7222 Tier II
5 NC_000010.11 10 93438923 + MYOF NNNACTGCCAGAGAACCTTG NGG 1 0.6429 Tier II
6 NC_000001.11 1 11056582 + SRM NNNGCTGCCAGAAAACCCTG NGG 2 0.5769 Tier II
7 NC_000010.11 10 48234548 + FRMPD2 NNNACTGACTGAGAACCCTG NGG 2 0.5735 Tier II
8 NC_000005.10 5 146735383 + PPP2R2B NNNACAGACAGAGAACCCTG NGG 2 0.5633 Tier II
9 NC_000004.12 4 51909653 + DCUN1D4 NNNACAGGCAGAGAACCCTG NGG 2 0.5571 Tier II
10 NC_000004.12 4 2950431 + NOP14 NNNGCTGCTAGAGAACCCTG NGG 2 0.5469 Tier II
11 NC_000004.12 4 185771882 + SORBS2 NNNACTGCCAGAGAACCATG NGG 1 0.5385 Tier II
12 NC_000019.10 19 10497286 + KEAP1 NNNAACGCCAGAGAACCCTG NGG 2 0.5195 Tier II
13 NC_000007.14 7 32533872 - AVL9 NNNACTGCCAGAGAACCATA NGG 2 0.5048 Tier II
14 NC_000003.12 3 156210742 - KCNAB1 NNNACTCCCAGGGAACCCTG NGG 2 0.4965 Tier II
15 NC_000011.10 11 10489508 - AMPD3 NNNACTGCAGGAGAACCCTG NGG 2 0.4762 Tier II
16 NC_000013.11 13 113465475 + DCUN1D2 NNNACTGCCAGAGAAAACTG NGG 2 0.4667 Tier II
17 NC_000023.11 X 100681502 - SYTL4 NNNACTGCCATAGAAACCTG NGG 2 0.3846 Tier II
18 NC_000015.10 15 100481851 + CERS3 NNNTGTGCCAGAGAACCCTG NGG 2 0.3818 Tier II
19 NC_000012.12 12 124098753 - ZNF664-RFLNA NNNACTTACAGAGAACCCTG NGG 2 0.3714 Tier II
20 NC_000008.11 8 40680637 - ZMAT4 NNNAATGGCAGAGAACCCTG NGG 2 0.3673 Tier II
21 NC_000003.12 3 119238957 + B4GALT4 NNNAGTGCCAGAGAACACTG NGG 2 0.28 Tier II
22 NC_000009.12 9 2032491 + SMARCA2 NNNACTGCCATGGAACCCTG NGG 2 0.2778 Tier II
23 NC_000005.10 5 160673052 - ATP10B NNNACTTCCACAGAACCCTG NGG 2 0.2449 Tier II
24 NC_000018.10 18 48546574 - CTIF NNNACTGCCTGAGATCCCTG NGG 2 0.1765 Tier II
25 NC_000017.11 17 50460995 + ACSF2 NNNGCTGCCAGAGAACCCCG NGG 2 0.1724 Tier II
26 NC_000008.11 8 635613 + ERICH1 NNNCCTGCCAGAGGACCCTG NGG 2 0.1647 Tier II
27 NC_000007.14 7 70537752 + AUTS2 NNNACTTCCAGAGAACCCTG NAG 2 0.1481 Tier II
28 NC_000002.12 2 239388961 + HDAC4 NNNACTGCCAGAGAACCCTG NCG 1 0.1071 Tier II
29 NC_000016.10 16 78658182 + WWOX NNNACTGCCAGATACCCCTG NGG 2 0.0818 Tier II
30 NC_000002.12 2 240781597 - KIF1A NNNACTGTCAGAGAACGCTG NGG 2 0.0515 Tier II
31 NC_000006.12 6 2740363 - MYLK4 NNNAATGCCAGAGAACCCTG NGA 2 0.0397 Tier II
32 NC_000015.10 15 22889318 - CYFIP1 NNNACTGCCAGAGCACCCTG NTG 2 0.0083 Tier II
33 NC_000004.12 4 2950431 + NOP14-AS1 NNNGCTGCTAGAGAACCCTG NGG 2 0.5469 Tier III
34 NC_000004.12 4 36641309 + LINC02505 NNNGCTGCCAGGGAACCCTG NGG 2 0.4514 Tier III
35 NC_000003.12 3 44478373 + LOC101928529 NNNAGTGCCAGAGAACCTTG NGG 2 0.3857 Tier III
36 NC_000014.9 14 38878987 - LINC00639 NNNACTTCCAGAGAGCCCTG NGG 2 0.3714 Tier III
37 NC_000014.9 14 38878987 - LOC105370457 NNNACTTCCAGAGAGCCCTG NGG 2 0.3714 Tier III
38 NC_000023.11 X 38924432 - LOC124905177 NNNTCTGCCAGAGAACACTG NGG 2 0.297 Tier III
39 NC_000003.12 3 119238957 + B4GALT4-AS1 NNNAGTGCCAGAGAACACTG NGG 2 0.28 Tier III
40 NC_000023.11 X 20620556 - LOC124905257 NNNACTGCCAAAGATCCCTG NGG 2 0.2 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 153007910 + Usp51 NNNACTGGCAGAGAAACCTG NGG 2 0.6429 Tier I
2 NC_000075.6 9 85688425 + Ibtk NNNACTGCCAGAGGCCCCTG NGG 2 0.1273 Tier I
3 NC_000076.6 10 120085450 - Grip1 NNNACTGATAGAGAACCCTG NGG 2 0.5688 Tier II
4 NC_000076.6 10 120085450 - Helb NNNACTGATAGAGAACCCTG NGG 2 0.5688 Tier II
5 NC_000082.6 16 8852044 - 1810013L24Rik NNNACAGCCAGAGAGCCCTG NGG 2 0.5633 Tier II
6 NC_000078.6 12 100839256 + Dglucy NNNTCTGCCAGAGAGCCCTG NGG 2 0.4136 Tier II
7 NC_000084.6 18 80879319 + Atp9b NNNGCTGGCAGAGAACCCTG NGG 2 0.4018 Tier II
8 NC_000074.6 8 41019781 + Mtus1 NNNTCTTCCAGAGAACCCTG NGG 2 0.3636 Tier II
9 NC_000075.6 9 108406350 + 1700102P08Rik NNNCCTGCCAGAGAACCCTG NGG 1 0.3529 Tier II
10 NC_000075.6 9 108406350 + Iho1 NNNCCTGCCAGAGAACCCTG NGG 1 0.3529 Tier II
11 NC_000083.6 17 27444348 + Grm4 NNNGCTGCCAGAGAACTCTG NGG 2 0.2917 Tier II
12 NC_000071.6 5 53209858 - Sel1l3 NNNACTGCCAGAAAATCCTG NGG 2 0.284 Tier II
13 NC_000085.6 19 4996375 - Npas4 NNNACTGCCAGAGAACCTTC NGG 2 0.2755 Tier II
14 NC_000082.6 16 93730438 + Dop1b NNNACTTCCAGAGAACACTG NGG 2 0.2667 Tier II
15 NC_000074.6 8 120742967 + Irf8 NNNACTGCCAACGAACCCTG NGG 2 0.2632 Tier II
16 NC_000078.6 12 116654684 + Ptprn2 NNNACTGCAAGAGAACCCAG NGG 2 0.2449 Tier II
17 NC_000070.6 4 137559287 - Hspg2 NNNACTGTCAGAGACCCCTG NGG 2 0.2386 Tier II
18 NC_000067.6 1 135915068 - Pkp1 NNNACTGCCAGGTAACCCTG NGG 2 0.2167 Tier II
19 NC_000080.6 14 119619362 + Hs6st3 NNNACTGCCAGACAACACTG NGG 2 0.1965 Tier II
20 NC_000070.6 4 63120199 + Zfp618 NNNACTGTCAGAGCACCCTG NGG 2 0.1875 Tier II
21 NC_000072.6 6 47531469 - Ezh2 NNNACTGACAGAGACCCCTG NGG 2 0.1773 Tier II
22 NC_000078.6 12 26368999 + Rnf144a NNNACTGCCATAGCACCCTG NGG 2 0.0824 Tier II
23 NC_000069.6 3 60599438 - Mbnl1 NNNACTGCCAAAGAACCCTG NGA 2 0.0694 Tier II
24 NC_000077.6 11 75398446 + Smyd4 NNNACTGCCAGAAAACCCTG NTG 2 0.036 Tier II
25 NC_000070.6 4 84619797 + Bnc2 NNNACTGCCAGGGAACCCTG NGT 2 0.0116 Tier II
26 NC_000074.6 8 41019781 + B430010I23Rik NNNTCTTCCAGAGAACCCTG NGG 2 0.3636 Tier III
27 NC_000078.6 12 26368999 + 4930549C15Rik NNNACTGCCATAGCACCCTG NGG 2 0.0824 Tier III
28 NC_000072.6 6 70718398 - Igk NNNACTGCCAGTGAAGCCTG NGG 2 0.0513 Tier III
29 NC_000072.6 6 70718398 - Gm46961 NNNACTGCCAGTGAAGCCTG NGG 2 0.0513 Tier III
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Other clones with same target sequence:

(none)